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This page provides an overview of all ISO standards referenced on the ISO homepage, per 02/04-2023.

ISO standards


Name Description Abstract Status Publication date Edition Number of pages Technical committee ICS
ISO 5985:2002/Cor 1:2005 Animal feeding stuffs — Determination of ash insoluble in hydrochloric acid — Technical Corrigendum 1  Published 2005-03 Edition : 2 Number of pages : 2 Technical Committee 65.120 Animal feeding stuffs
ISO 6490-1:1985 Animal feeding stuffs — Determination of calcium content — Part 1: Titrimetric method The method is applicable to products having contents greater than 1 g/kg. It consists in ashing of a test portion, treatment of the ash with hydrochloric acid and precipitation of the calcium as calcium oxalate, dissolution of the precipitation in sulfuric acid and titration with standard volumetric potassium permanganate solution of the oxalic acid formed.  Published 1985-11 Edition : 1 Number of pages : 2 Technical Committee 65.120 Animal feeding stuffs
ISO 6490-2:1983 Animal feeding stuffs — Determination of calcium content — Part 2: Atomic absorption spectrometric method The limit of detection is in the region of 10 mg/kg. The principle of the method is, following any necessary destruction of organic matter by incineration of a test portion, dissolution of the calcium by treatment with hydrochloric acid and dilution of the solution obtained, in the presence of lanthanum. Then determination of the calcium content by atomic absorption spectrometry.  Withdrawn 1983-11 Edition : 1 Number of pages : 3 Technical Committee 65.120 Animal feeding stuffs
ISO 6491:1980 Animal feeding stuffs — Determination of total phosphorus content — Spectrophotometric method  Withdrawn 1980-08 Edition : 1 Number of pages : 3 Technical Committee 65.120 Animal feeding stuffs
ISO 6491:1998 Animal feeding stuffs — Determination of phosphorus content — Spectrometric method This International Standard specifies a spectrometric method for the determination of the phosphorus content of animal feeding stuffs. The method is applicable to animal feeding stuffs with a phosphorus content less than 50 g/kg. It is particularly appropriate for the analysis of products with low phosphorus content. For products with higher phosphorus content, application of a gravimetric method is advised, using for instance quinoline phosphomolybdate.  Published 1998-12 Edition : 2 Number of pages : 7 Technical Committee 65.120 Animal feeding stuffs
ISO 6492:1999 Animal feeding stuffs — Determination of fat content This International Standard specifies a method for the determination of the fat content of animal feeding stuffs. The method is applicable to animal feeding stuffs except oilseeds and oilseed residues. For the purposes of this method, the following two categories of animal feeding stuffs are distinguished. Samples of products in category B need a hydrolysis step prior to extraction. Category B: straight feeds of animal origin including milk products; straight feeds of vegetable origin from which fats cannot be extracted without prior hydrolysis; in particular: gluten, yeast, soya and potato proteins, and heat-treated feeds; compound feeds containing the preceding products in such quantities that at least 20 % of the fat content stems from these products. Category A: animal feeding stuffs not mentioned under category B. NOTE For oilseed residues, a method for the determination of the "oil content" by hexane extraction is specified in ISO 734-1 [2], whereas a method for the determination of the "oil content" by diethyl ether extraction is specified in ISO 736 [3]. For oilseeds, a method for the determination of the "oil content" by hexane extraction is specified in ISO 659 [1].  Published 1999-08 Edition : 1 Number of pages : 9 Technical Committee 65.120 Animal feeding stuffs
ISO 6493:2000 Animal feeding stuffs — Determination of starch content — Polarimetric method  Published 2000-02 Edition : 1 Number of pages : 10 Technical Committee 65.120 Animal feeding stuffs
ISO 6495-1:2015 Animal feeding stuffs — Determination of water-soluble chlorides content — Part 1: Titrimetric method ISO 6495-1:2015 specifies a method for the determination of water-soluble chloride content, expressed as sodium chloride, of animal feeding stuffs. This method is applicable to animal feeding stuffs containing water-soluble chloride content, expressed as sodium chloride, ≥0,05 %.  Published 2015-06 Edition : 1 Number of pages : 9 Technical Committee 65.120 Animal feeding stuffs
ISO 6495:1980 Animal feeding stuffs — Determination of water-soluble chlorides content  Withdrawn 1980-09 Edition : 1 Number of pages : 3 Technical Committee 65.120 Animal feeding stuffs
ISO 6495:1999 Animal feeding stuffs — Determination of water-soluble chlorides content  Withdrawn 1999-04 Edition : 2 Number of pages : 7 Technical Committee 65.120 Animal feeding stuffs
ISO 6496:1983 Animal feeding stuffs — Determination of moisture content  Withdrawn 1983-08 Edition : 1 Number of pages : 3 Technical Committee 65.120 Animal feeding stuffs
ISO 6866:1985 Animal feeding stuffs — Determination of free and total gossypol The method is applicable to cottonseed and cottonseed meals and cakes. The method consists in extracting of the compound in the presence of 3-aminopropane-1-ol, either with a mexture of 2-propanol and hexane for the determination of free gossypol or with dimethylformamide for the determination of total gossypol. Conversion to gossypol-dianiline using aniline, and measurement of the absorbance at the wavelength of maximum absorbance (between 435 and 445 nm).  Published 1985-11 Edition : 1 Number of pages : 3 Technical Committee 65.120 Animal feeding stuffs
ISO 6496:1999 Animal feeding stuffs — Determination of moisture and other volatile matter content This International Standard specifies a method for the determination of the moisture and other volatile matter content of animal feeding stuffs. The method is applicable to animal feeding stuffs with the exception of: a) milk products; b) mineral substances; c) mixtures containing a considerable amount of milk products or mineral substances, for example milk replacers; d) animal feeding stuffs containing humectants (e.g. propylene glycol); e) the following simple animal feeding stuffs: animal and vegetable fats and oils (for which method A specified in ISO 662 [1] is applicable); oilseeds (for which a method is specified in ISO 665 [2]); oilseed residues (for which a method is specified in ISO 771 [3]); cereals, except maize, and cereal products (for which a method is specified in ISO 712 [4]); maize (for which the reference method specified in ISO 6540 [5] is applicable)  Published 1999-08 Edition : 2 Number of pages : 7 Technical Committee 65.120 Animal feeding stuffs
ISO 6497:2002 Animal feeding stuffs — Sampling ISO 6497:2002 specifies methods of sampling animal feeding stuffs, including fish feed, for quality control for commercial, technical and legal purposes. It is not applicable to pet foods. Nor are the methods intended for sampling for the purpose of microbiological examination. Conditions of, and requirements for, sampling are specified separately for feeding stuffs of different physical natures. For certain categories of animal feeding stuff, specific methods of sampling are specified in other International Standards. A list of these can be found in the bibliography. When sampling the products specified, it is these methods which shall be used. Methods of sampling for the determination of substances likely to be non-uniformly distributed are described in an annex.  Published 2002-11 Edition : 1 Number of pages : 19 Technical Committee 65.120 Animal feeding stuffs
ISO 6498:1983 Animal feeding stuffs — Preparation of test samples  Withdrawn 1983-10 Edition : 1 Number of pages : 6 Technical Committee 65.120 Animal feeding stuffs
ISO 6498:1998 Animal feeding stuffs — Preparation of test samples  Withdrawn 1998-11 Edition : 2 Number of pages : 8 Technical Committee 65.120 Animal feeding stuffs
ISO 6498:2012 Animal feeding stuffs — Guidelines for sample preparation ISO 6498:2012 specifies guidelines for the preparation of test samples from laboratory samples of animal feeding stuffs, including pet foods. The guidelines are overruled by special instructions and regulations for sample preparation demanded by specific analysis methods.  Published 2012-06 Edition : 3 Number of pages : 46 Technical Committee 65.120 Animal feeding stuffs
ISO 6651:1983 Animal feeding stuffs — Determination of aflatoxin B1 content  Withdrawn 1983-04 Edition : 1 Number of pages : 8 Technical Committee 65.120 Animal feeding stuffs
ISO 6651:1987 Animal feeding stuffs — Determination of aflatoxin B1 content Describes two methods of determination for different fields of application. Method A is applicable to simple feeding stuffs while method B can be used for mixed feeding stuffs and for feeding stuffs not covered by method A. Contains information on the principle of determination, reagents and apparatus to be used, sampling, procedure, expression of results and the details to be included in the test report.  Withdrawn 1987-08 Edition : 2 Number of pages : 8 Technical Committee 65.120 Animal feeding stuffs
ISO 6651:2001 Animal feeding stuffs — Semi-quantitative determination of aflatoxin B1 — Thin-layer chromatographic methods  Withdrawn 2001-12 Edition : 3 Number of pages : 13 Technical Committee 65.120 Animal feeding stuffs
ISO 6654:1991 Animal feeding stuffs — Determination of urea content Specifies a spectrometric method for the determination of the urea content. Gives a normative reference to ISO 6498:1983. Defines the term urea content. Describes principle, reagents, apparatus, sampling, preparation of the test sample, procedure, expression of results and test report.  Published 1991-03 Edition : 1 Number of pages : 3 Technical Committee 65.120 Animal feeding stuffs
ISO 6655:1997 Animal feeding stuffs — Determination of soluble nitrogen content after treatment with pepsin in dilute hydrochloric acid  Published 1997-08 Edition : 1 Number of pages : 10 Technical Committee 65.120 Animal feeding stuffs
ISO 6865:2000 Animal feeding stuffs — Determination of crude fibre content — Method with intermediate filtration Cette norme internationale prescrit une méthode de détermination de la teneur en cellulose brute par filtration intermédiaire. Elle décrit un mode opératoire manuel et un mode opératoire semi-automatique. Cette méthode est applicable aux aliments des animaux ayant une teneur en cellulose brute supérieure à 10 g/kg. NOTE Pour des aliments pour animaux contenant de la cellulose brute inférieure ou égale à 10 g/kg, la méthode décrite dans l'ISO 6541 doit être utilisée. Cette Norme internationale est également applicable aux céréales et aux légumes.»  Published 2000-10 Edition : 1 Number of pages : 10 Technical Committee 65.120 Animal feeding stuffs
ISO 4832:1978 Microbiology — General guidance for enumeration of coliforms — Colony count technique at 30 degrees C  Withdrawn 1978-02 Edition : 1 Number of pages : 4 Technical Committee 07.100.30 Food microbiology
ISO 6867:2000 Animal feeding stuffs — Determination of vitamin E content — Method using high-performance liquid chromatography This International Standard specifies a method for the determination of the vitamin E (DL-_-tocopherol) content of animal feeding stuffs and pet foods using high performance liquid chromatography.  Published 2000-12 Edition : 1 Number of pages : 11 Technical Committee 65.120 Animal feeding stuffs
ISO 6869:2000 Animal feeding stuffs — Determination of the contents of calcium, copper, iron, magnesium, manganese, potassium, sodium and zinc — Method using atomic absorption spectrometry  Published 2000-12 Edition : 1 Number of pages : 15 Technical Committee 65.120 Animal feeding stuffs
ISO 6870:1985 Animal feeding stuffs — Determination of zearalenone content The limit of determination is approximately 50 micro-gram/kg. The method consists in extracting of a test portion with a mixture of acetonitrile and potassium chloride solution, filtrating, defatting, purifying, filtrating again, extracting into chloroform which is subsequently evaporated. Dissolution of the dry extract in a mixture of benzene and acetonitrile and thin layer chromatography. Determination by visual measurement or by measurement of the intensity of fluorescence under UV ligt by comparison with known quantities of zearalenone applied to the same plate. Confirmation using bis-diazotized benzidine reagent.  Withdrawn 1985-11 Edition : 1 Number of pages : 5 Technical Committee 65.120 Animal feeding stuffs
ISO 6870:2002 Animal feeding stuffs — Qualitative determination of zearalenone This International Standard specifies a qualitative method for the determination of zearalenone in animal feeding stuffs and, in particular, in maize. This method is for screening purposes only. The limit of determination of zearalenone is approximately 50 μg/kg . NOTE Although sorghum gives interfering fluorescent spots identical to those of zearalenone, the method is still applicable to this feed because Rf the values are different after development of the chromatogram in the second direction. These spots are not developed by the specified confirmation technique.  Published 2002-04 Edition : 2 Number of pages : 8 Technical Committee 65.120 Animal feeding stuffs
ISO 7088:1981 Fish-meal — Vocabulary Defines terms concerning fish-meal, in English and French. An alphabetical index of the French terms is not included.  Published 1981-10 Edition : 1 Number of pages : 2 Technical Committee 65.120 Animal feeding stuffs ; 01.040.65 Agriculture (Vocabularies)
ISO 7485:2000 Animal feeding stuffs — Determination of potassium and sodium contents — Methods using flame-emission spectrometry This International Standard specifies a calibration method and a standard addition method for the determination of potassium and sodium contents of animal feeding stuffs by flame-emission spectrometry. The standard addition method applies if it is not known whether the emissions measured for potassium or sodium are subject to matrix effects. The limit of determination for potassium and sodium is 0,04 g/kg. The calibration method applies if such matrix effects have been shown to be absent.  Published 2000-08 Edition : 1 Number of pages : 10 Technical Committee 65.120 Animal feeding stuffs
ISO 7555:1987 Soya beans — Specification  Withdrawn 1987-12 Edition : 1 Number of pages : 2 Technical Committee 65.120 Animal feeding stuffs ; 67.200.20 Oilseeds
ISO 9831:1998 Animal feeding stuffs, animal products, and faeces or urine — Determination of gross calorific value — Bomb calorimeter method  Published 1998-08 Edition : 1 Number of pages : 23 Technical Committee 65.120 Animal feeding stuffs
ISO 12099:2010 Animal feeding stuffs, cereals and milled cereal products — Guidelines for the application of near infrared spectrometry ISO 12099:2010 gives guidelines for the determination by near infrared spectroscopy of constituents such as moisture, fat, protein, starch, and crude fibre as well as parameters such as digestibility in animal feeding stuffs, cereals and milled cereal products. The determinations are based on spectrometric measurement in the near infrared spectral region.  Withdrawn 2010-06 Edition : 1 Number of pages : 30 Technical Committee 65.120 Animal feeding stuffs
ISO 12099:2017 Animal feeding stuffs, cereals and milled cereal products — Guidelines for the application of near infrared spectrometry ISO 12099:2017 gives guidelines for the determination by near infrared spectroscopy of constituents such as moisture, fat, protein, starch and crude fibre and parameters such as digestibility in animal feeding stuffs, cereals and milled cereal products. The determinations are based on spectrometric measurement in the near infrared spectral region.  Published 2017-08 Edition : 2 Number of pages : 28 Technical Committee 65.120 Animal feeding stuffs
ISO 6887-1:1999 Microbiology of food and animal feeding stuffs — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 1: General rules for the preparation of the initial suspension and decimal dilutions  Withdrawn 1999-02 Edition : 1 Number of pages : 5 Technical Committee 07.100.30 Food microbiology
ISO 13903:2005 Animal feeding stuffs — Determination of amino acids content ISO 13903:2005 describes the determination of free (synthetic and natural) and total (peptide-bound and free) amino acids in feeding stuffs, using an amino acid analyser or HPLC equipment. It is applicable to the following amino acids: sum of cystine and cysteine; methionine; lysine; threonine; alanine; arginine; aspartic acid; glutamic acid; glycine; histidine; isoleucine; leucine; phenylalanine; proline; serine; tyrosine; valine. The method does not distinguish between the salts of amino acids, nor does it differentiate between D and L forms of amino acids. It is not valid for the determination of tryptophan or hydroxy analogues of amino acids. Limits of quantification depend on the chromatographic equipment, but levels as low as: 0,3 g/kg total lysine; 0,25 g/kg total methionine; 0,35 g/kg total cystine plus cysteine; 0,2 g/kg total threonine; 0,035g/kg free lysine; 0,035g/kg free methionine; and 0,03g/kg free threonine can typically be analysed.  Published 2005-05 Edition : 1 Number of pages : 17 Technical Committee 65.120 Animal feeding stuffs
ISO 13904:2005 Animal feeding stuffs — Determination of tryptophan content ISO 13904:2005 describes the determination of the total and free tryptophan in feeding stuffs (e.g. complete and complementary feeds, supplementary feeds, raw materials, ingredients, premixtures and concentrates. It does not distinguish between D- and L-forms.  Withdrawn 2005-05 Edition : 1 Number of pages : 9 Technical Committee 65.120 Animal feeding stuffs
ISO 13904:2016 Animal feeding stuffs — Determination of tryptophan content ISO 13904:2016 specifies a method for determination of the total and free tryptophan (Trp) content in feeding stuffs (e.g. complete and complementary feeds, supplementary feeds, raw materials, ingredients, and concentrates) and determination of free tryptophan in commercial pure substances and premixtures containing more than 2 % of tryptophan. It does not distinguish between D- and L-forms.  Published 2016-02 Edition : 2 Number of pages : 14 Technical Committee 65.120 Animal feeding stuffs
ISO 13906:2008 Animal feeding stuffs — Determination of acid detergent fibre (ADF) and acid detergent lignin (ADL) contents ISO 13906:2008 specifies a method for the determination of acid detergent fibre (ADF) insoluble residue and acid detergent lignin (ADL) in all types of animal feeding stuffs. The limit of determination is 1 % mass fraction for ADF and 1,5 % mass fraction for ADL. A gravimetric routine and reference method is used.  Published 2008-07 Edition : 1 Number of pages : 17 Technical Committee 65.120 Animal feeding stuffs
ISO 14181:2000 Animal feeding stuffs — Determination of residues of organochlorine pesticides — Gas chromatographic method This International Standard specifies a gas chromatographic method for the determination of residues of organochlorine pesticides in animal feeding stuffs. The method is applicable to animal feeding stuffs containing residues of one or more of the following organochlorine pesticides and some of their isomers and degradation products: aldrin, op'-DDE, pp'-DDE, op'-DDT, pp'-DDT, dieldrin, endosulfan, endrin, HCB, _-HCH (_-BHC), _-HCH (_-BHC), _-HCH (_-BHC), _-HCH (_-BHC), heptachlor, heptachlor epoxide, op'-TDE (op'-DDD), pp'-TDE (pp'-DDD) and methoxychlor. The lower determination limit for these organochlorine pesticides is 0,005 _g/g. However, the lower determination limit is 0,01 _g/g for op'-DDT and pp'-DDT, and 0,05 _g/g for methoxychlor.  Published 2000-09 Edition : 1 Number of pages : 20 Technical Committee 65.120 Animal feeding stuffs
ISO 14182:1999 Animal feeding stuffs — Determination of residues of organophosphorus pesticides — Gas chromatographic method  Published 1999-12 Edition : 1 Number of pages : 18 Technical Committee 65.120 Animal feeding stuffs
ISO 14183:2005 Animal feeding stuffs — Determination of monensin, narasin and salinomycin contents — Liquid chromatographic method using post-column derivatization ISO 14183:2005 specifies a high-performance liquid chromatographic (HPLC) method for the determination of the monensin, narasin and salinomycin contents of animal feeding stuffs, supplements (dry and liquid) and mineral premixtures. The method is not applicable to drug premixes (pharmaceutical products). Lasalocid and semduramicin cannot be determined by this method. The limit of quantitation is approximately 1 mg/kg, 2 mg/kg and 2 mg/kg for monensin, salinomycin and narasin, respectively. A lower limit of quantitation can be achievable but this is to be validated by the user.  Published 2005-11 Edition : 1 Number of pages : 21 Technical Committee 65.120 Animal feeding stuffs
ISO 14183:2005/Cor 1:2010 Animal feeding stuffs — Determination of monensin, narasin and salinomycin contents — Liquid chromatographic method using post-column derivatization — Technical Corrigendum 1  Published 2010-04 Edition : 1 Technical Committee 65.120 Animal feeding stuffs
ISO 14565:2000 Animal feeding stuffs — Determination of vitamin A content — Method using high-performance liquid chromatography  Published 2000-12 Edition : 1 Number of pages : 11 Technical Committee 65.120 Animal feeding stuffs
ISO 6888-2:1999 Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) — Part 2: Technique using rabbit plasma fibrinogen agar medium  Withdrawn 1999-02 Edition : 1 Number of pages : 7 Technical Committee 07.100.30 Food microbiology
ISO 14718:1998 Animal feeding stuffs — Determination of aflatoxin B1 content of mixed feeding stuffs — Method using high-performance liquid chromatography This International Standard specifies a high-performance liquid chromatographic (HPLC) method for the determination of aflatoxin B1 content of animal feeding stuffs including those containing citrus pulp. The lower limit of determination is 1 mg/kg. NOTE 1 This International Standard may be applicable for the determination of the aflatoxin B1 content of a number of raw materials and straight feeding stuffs such as corn gluten, groundnut, palm kernel, copra, citrus pulp, tapioca, soya bean, rice bran, pollard, rape seed, niger seed and cotton seed (see references [1] and [2]). These materials were, however, not included in the collaborative testing of the method. NOTE 2 This International Standard may also be applicable for the determination of the content of the sum of the aflatoxins B1, B2, G1 and G2. However, the method has not been validated for this parameter by collaborative testing.  Published 1998-12 Edition : 1 Number of pages : 16 Technical Committee 65.120 Animal feeding stuffs
ISO 14797:1999 Animal feeding stuffs — Determination of furazolidone content — Method using high-performance liquid chromatography This International Standard specifies a high-performance liquid chromatographic (HPLC) method for the determination of the furazolidone content of premixtures and animal feeding stuffs. The method is applicable to animal feeding stuffs with a furazolidone content of 25 mg/kg to 5 000 mg/kg and to premixtures with a mass fraction of furazolidone of up to 20 % [formerly written as 20 % (m/m)]. NOTE 1 For animal feeding stuffs, the furazolidone content is expressed in milligrams per kilogram; for premixtures, as a mass fraction in percent [% (m/m)]. NOTE 2 Furazolidone is a chemotherapeuticum belonging to the group of nitrofuranes. Nitrofuranes are bacteriostatic or bactericidal against Gram-positive and Gram-negative microorganisms and against some moulds and protozoa.  Published 1999-03 Edition : 1 Number of pages : 13 Technical Committee 65.120 Animal feeding stuffs
ISO 14902:2001 Animal feeding stuffs — Determination of trypsin inhibitor activity of soya products This International Standard specifies a method for the determination of the trypsin inhibitor activity (TIA) of soya products. This trypsin inhibitor activity is indicative of the degree of toasting of these products. The detection limit of the method is 0,5 mg/g.  Published 2001-10 Edition : 1 Number of pages : 11 Technical Committee 65.120 Animal feeding stuffs
ISO 14939:2001 Animal feeding stuffs — Determination of carbadox content — Method using high-performance liquid chromatography This International Standard specifies a high-performance liquid chromatographic (HPLC) method for the determination of the carbadox content in premixtures and animal feeding stuffs. The method is applicable to animal feeding stuffs with a mass fraction of carbadox of 0,5 mg/kg (limit of quantification) to 100 mg/kg, and to premixtures with a mass fraction of carbadox up to 10 %. The lower limit of detection is 0,1 mg/kg. NOTE 1 For animal feeding stuffs the mass fraction of carbadox is expressed in milligrams per kilogram, and for premixtures as a percentage by mass. NOTE 2 Carbadox is a chemotherapeuticum belonging to the quinoxaline group. Carbadox is used as a growth-promoting feed additive for piglets.  Published 2001-08 Edition : 1 Number of pages : 16 Technical Committee 65.120 Animal feeding stuffs
ISO/DIS 15914-2 Animal feeding stuffs — Enzymatic determination of starch — Part 2: Method by enzymatic determination with a hexokinase system and potassium hydroxide dispersion This document describes an enzymatic method for determining starch in animal feeding stuffs containing starchy ingredients (cereals, tubers, etc.). The method also applies to beans and to the animal digestive contents because it involves the hexokinase system for the final glucose determination.  Under development Edition : 1 Technical Committee 65.120 Animal feeding stuffs
ISO 15914:2004 Animal feeding stuffs — Enzymatic determination of total starch content ISO 15914:2004 specifies a method for the enzymatic determination of the total starch content of animal feeding stuffs and raw materials for animal feeding stuffs. The method is also applicable to the determination of starch in starch. It is important that in the sample matrix no components are present which contribute to the measured extinction at 340 nm. The analytical range of the method is 40 g/kg to 1 000 g/kg starch. The standard procedure is applicable to the range 200 g/kg to 1000 g/kg. For the lower range, 40 g/kg to 200 g/kg, another dilution procedure for the standard glucose solution and samples can be used.  Published 2004-02 Edition : 1 Number of pages : 12 Technical Committee 65.120 Animal feeding stuffs
ISO 16472:2006 Animal feeding stuffs — Determination of amylase-treated neutral detergent fibre content (aNDF) ISO 16472:2006 specifies methods for the determination of amylase-treated neutral detergent insoluble fibrous residue content in all types of animal feed. It includes a gravimetric routine method and a reference method.  Published 2006-04 Edition : 1 Number of pages : 16 Technical Committee 65.120 Animal feeding stuffs
ISO 4832:1991 Microbiology — General guidance for the enumeration of coliforms — Colony count technique This second edition cancels and replaces the first edition (ISO 4832:1978). It is intended to provide general guidance for the examination of products not dealt with by existing International Standards. These guidelines may not be appropriate for some products in every detail, and for some other products it may be necessary to use different methods. Gives references, and the definition of the term coliforms. Specifies principle, culture media and dilution fluid, apparatus and glassware, sampling, preparation of the test sample, procedure, expression of results and test report.  Withdrawn 1991-03 Edition : 2 Number of pages : 5 Technical Committee 07.100.30 Food microbiology
ISO 16634-1:2008 Food products - Determination of the total nitrogen content by combustion according to the Dumas principle and calculation of the crude protein content — Part 1: Oilseeds and animal feeding stuffs ISO 16634-1:2008 specifies a method for the determination of the total nitrogen content and the calculation of crude protein content of oilseeds and animal feeding stuffs. This method, like the Kjeldahl method, does not distinguish between protein nitrogen and non-protein nitrogen. For the calculation of protein content, various conversion factors are used. This method is not applicable to milk and milk products.  Published 2008-11 Edition : 1 Number of pages : 30 Technical Committee 65.120 Animal feeding stuffs ; 67.050 General methods of tests and analysis for food products ; 67.200.20 Oilseeds
ISO 17180:2013 Animal feeding stuffs — Determination of lysine, methionine and threonine in commercial amino acid products and premixtures ISO 17180:2013 specifies a method for the quantitative determination of free (non-protein-bound) lysine, methionine, and threonine in commercial products and premixtures containing more than about 10 % mass fraction of the respective amino acid. It does not distinguish between d- and l-forms.  Published 2013-04 Edition : 1 Number of pages : 11 Technical Committee 65.120 Animal feeding stuffs
ISO 17372:2008 Animal feeding stuffs — Determination of zearalenone by immunoaffinity column chromatography and high performance liquid chromatography ISO 17372:2007 is applicable to the analysis of zearalenone in animal feed and feed ingredients, including barley, corn, oats, rye, wheat, soybean meal, canola (rapeseed) meal, corn gluten, dried distillers' grains, lentils, and sugar beet pulp. The limit of quantification is 0,05 mg/kg (50 µg/kg). A lower limit of quantification may be achievable subject to appropriate validation being conducted by the user laboratory.  Published 2008-02 Edition : 1 Number of pages : 16 Technical Committee 65.120 Animal feeding stuffs
ISO 17372:2008/Amd 1:2013 Animal feeding stuffs — Determination of zearalenone by immunoaffinity column chromatography and high performance liquid chromatography — Amendment 1: Limitation of the scope  Published 2013-02 Edition : 1 Number of pages : 1 Technical Committee 65.120 Animal feeding stuffs
ISO 17375:2006 Animal feeding stuffs — Determination of aflatoxin B1 ISO 17375:2006 specifies a method for the determination of aflatoxin B1 in animal feeding stuffs using high-performance liquid chromatography with post-column derivatization. It is applicable to animal feeding stuffs with a fat content of up to 50 %. The limit of quantification of this method has been demonstrated to be better than 0,5 g/kg for aflatoxin B1 for a signal-to-noise ratio of 6.  Published 2006-06 Edition : 1 Number of pages : 11 Technical Committee 65.120 Animal feeding stuffs
ISO/TS 17764-1:2002 Animal feeding stuffs — Determination of the content of fatty acids — Part 1: Preparation of methyl esters ISO/TS 17764-1:2002 specifies two methods for preparing the methyl esters of fatty acids of animal and vegetable fats, oils and fatty acid mixtures for raw materials for compound animal feeds, and fatty acids originating from fat extracts of animal feeding stuffs, including fats and fatty acid mixtures containing butyric acid. The general method, the boron trifluoride (BF3) method is concerned with the preparation of methyl esters of fatty acids with six or more C atoms, originating from fats, oils and free fatty acids. The KOH/HCl method is concerned with the preparation of methyl esters of fatty acids with four or more C atoms. This method can also be used for the quantitative determination of fatty acids with a chain length shorter than 10 C atoms in free fatty acid mixtures. The methyl esters produced can be used for gas-liquid chromatography (GLC). Unsaponifiable materials are not removed and can, when present in considerable amounts, interfere with the chromatographic analysis. ISO/TS 17764-2:2002 describes the application of gas chromatography with capillary columns and flame ionization detection for the determination of the content of fatty acids in a fat by making use of the methyl esters of the fatty acids obtained in accordance with the methods specified in ISO/TS 17764-1:2002.  Published 2002-11 Edition : 1 Number of pages : 6 Technical Committee 65.120 Animal feeding stuffs
ISO/TS 17764-2:2002 Animal feeding stuffs — Determination of the content of fatty acids — Part 2: Gas chromatographic method ISO/TS 17764-2:2002 specifies the application of gas chromatography with capillary columns and flame ionization detection for the determination of the quantitative content of fatty acids in a fat by making use of the methyl esters of the fatty acids obtained in accordance with the method specified in ISO/TS 17764-1:2002. ISO/TS 17764-2:2002 is applicable to the investigation of animal and vegetable fats, oils and fatty acid mixtures for incorporation in animal feeding stuffs and fat extracts of animal feeding stuffs and raw materials for compound animal feeds, including fats and fatty acid mixtures containing butyric acid. This method is not applicable to polymerized fatty acids.  Published 2002-11 Edition : 1 Number of pages : 10 Technical Committee 65.120 Animal feeding stuffs
ISO 20588:2019 Animal feeding stuffs — Vocabulary This document defines terms relating to animal feeding stuffs. NOTE It includes the most common and frequently used terms in the field of animal feeding stuffs.  Published 2019-10 Edition : 1 Number of pages : 14 Technical Committee 65.120 Animal feeding stuffs ; 01.040.65 Agriculture (Vocabularies)
ISO 27085:2009 Animal feeding stuffs — Determination of calcium, sodium, phosphorus, magnesium, potassium, iron, zinc, copper, manganese, cobalt, molybdenum, arsenic, lead and cadmium by ICP-AES ISO 27085:2009 specifies an inductively coupled plasma atomic emission spectroscopic (ICP-AES) method for the determination of: a) the minerals calcium, sodium, phosphorus, magnesium and potassium and the elements iron, zinc, copper, manganese, cobalt and molybdenum in animal feeding stuffs; b) the elements arsenic, lead and cadmium in minerals on their own, in premixtures or mixtures for use in animal feeding stuffs. The method detection limit for each element is dependent on the sample matrix as well as on the instrument. The method is not applicable for determination of low concentrations of elements. The limit of quantification is 3 mg/kg or lower.  Withdrawn 2009-04 Edition : 1 Number of pages : 24 Technical Committee 65.120 Animal feeding stuffs
ISO 30024:2009 Animal feeding stuffs — Determination of phytase activity ISO 30024:2009 specifies the determination of phytase activity in feed samples. The method does not distinguish between phytase added as a feed additive and endogenous phytase already present in the feed materials. The method cannot be used to evaluate or compare the in vivo efficacy of the phytase product. It is not a predictive method of the in vivo efficacy of phytases present on the market as they can develop different in vivo efficacy per unit of activity. The method is suitable and validated exclusively for the determination of phytase activity and exclusively in complete feeds.  Published 2009-07 Edition : 1 Number of pages : 12 Technical Committee 65.120 Animal feeding stuffs
ISO/DIS 30024 Animal feeding stuffs — Determination of phytase activity  Under development Edition : 2 Number of pages : 22 Technical Committee 65.120 Animal feeding stuffs
ISO 2293:1976 Meat and meat products — Aerobic count at 30 degrees C (Reference method)  Withdrawn 1976-04 Edition : 1 Number of pages : 3 Technical Committee 07.100.30 Food microbiology
ISO 2293:1988 Meat and meat products — Enumeration of micro-organisms — Colony count technique at 30 degrees C (Reference method)  Withdrawn 1988-08 Edition : 2 Number of pages : 4 Technical Committee 07.100.30 Food microbiology
ISO 3100-2:1988 Meat and meat products — Sampling and preparation of test samples — Part 2: Preparation of test samples for microbiological examination The method gives general instructions and specifies procedures to be followed after taking a laboratory sample. This may require the thawing and/or mincing of "open" samples or the pre-incubation, external sterilisation, and aseptic opening of products processed or packed in sealed units.  Withdrawn 1988-06 Edition : 1 Number of pages : 4 Technical Committee 07.100.30 Food microbiology
ISO 3565:1975 Meat and meat products — Detection of salmonellae (Reference method)  Withdrawn 1975-09 Edition : 1 Number of pages : 11 Technical Committee 07.100.30 Food microbiology
ISO 3811:1979 Meat and meat products — Detection and enumeration of presumptive coliform bacteria and presumptive Escherichia coli — (Reference method)  Withdrawn 1979-11 Edition : 1 Number of pages : 5 Technical Committee 07.100.30 Food microbiology
ISO 4031:1978 Information interchange — Representation of local time differentials  Withdrawn 1978-12 Edition : 1 Number of pages : 2 Technical Committee 07.100.30 Food microbiology
ISO 4831:1978 Microbiology — General guidance for the enumeration of coliforms — Most probable number technique at 30 degrees C  Withdrawn 1978-08 Edition : 1 Number of pages : 8 Technical Committee 07.100.30 Food microbiology
ISO 4831:1991 Microbiology — General guidance for the enumeration of coliforms — Most probable number technique This second edition cancels and replaces the first edition (ISO 4831:1978). It is intended to provide general guidance for the examination of products not dealt with by existing International Standards. These guidelines may not be appropriate for some products in every detail, and for some other products it may be necessary to use different methods. The technique described here is less precise than that described in ISO 4832. Gives references, and the definition of the term coliforms. Specifies principle, culture media and dilution fluid, apparatus and glassware, sampling, preparation of the test sample, procedure, expression of results and test report.  Withdrawn 1991-03 Edition : 2 Number of pages : 11 Technical Committee 07.100.30 Food microbiology
ISO 4831:2006 Microbiology of food and animal feeding stuffs — Horizontal method for the detection and enumeration of coliforms — Most probable number technique ISO 4831:2006 gives general guidelines for the detection and the enumeration of coliforms. It is applicable to products intended for human consumption and for the feeding of animals, and to environmental samples in the area of food production and food handling. Enumeration is carried out by calculation of the most probable number (MPN) after incubation in a liquid medium at 30 °C or 37 °C. This enumeration method is applicable when the number sought is expected to be in the range 1 to 100 per millilitre or per gram of test sample.  Published 2006-08 Edition : 3 Number of pages : 11 Technical Committee 07.100.30 Food microbiology
ISO 4832:2006 Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of coliforms — Colony-count technique ISO 4832:2006 gives general guidelines for the enumeration of coliforms. It is applicable to products intended for human consumption and for the feeding of animals, and environmental samples in the area of food production and food handling, by means of the technique of counting colonies after incubation on a solid medium at 30 °C or at 37 °C. This technique is recommended when the number of colonies sought is expected to be more than 100 per millilitre or per gram of the test sample.  Published 2006-02 Edition : 3 Number of pages : 6 Technical Committee 07.100.30 Food microbiology
ISO 4833-1:2013 Microbiology of the food chain — Horizontal method for the enumeration of microorganisms — Part 1: Colony count at 30 °C by the pour plate technique ISO 4833-1:2013 specifies a horizontal method for enumeration of microorganisms that are able to grow and form colonies in a solid medium after aerobic incubation at 30 °C. The method is applicable to: a) products intended for human consumption and for animal feed; b) environmental samples in the area of food and feed production and handling. ISO 4833-1:2013 is applicable to: 1) products that require a reliable count when a low limit of detection is specified (below 102/g or 102/ml for liquid samples or below 103/g for solid samples); 2) products expected to contain spreading colonies that obscure colonies of other organisms, e.g. milk and milk products likely to contain spreading Bacillus spp. The applicability of ISO 4833-1:2013 to the examination of certain fermented food and animal feeds is limited and other media or incubation conditions can be more appropriate. However, this method can be applied to such products even though it is possible that the predominant microorganisms in those products are not detected effectively. For some matrices, the method specified in ISO 4833-1:2013 can give different results to those obtained using the method specified in ISO 4833‑2.  Published 2013-09 Edition : 1 Number of pages : 9 Technical Committee 07.100.30 Food microbiology
ISO 4833-1:2013/Amd 1:2022 Microbiology of the food chain — Horizontal method for the enumeration of microorganisms — Part 1: Colony count at 30 °C by the pour plate technique — Amendment 1: Clarification of scope  Published 2022-01 Edition : 1 Number of pages : 1 Technical Committee 07.100.30 Food microbiology
ISO 4833-2:2013 Microbiology of the food chain — Horizontal method for the enumeration of microorganisms — Part 2: Colony count at 30 °C by the surface plating technique ISO 4833-2:2013 specifies a horizontal method for enumeration of microorganisms that are able to grow and form colonies on the surface of a solid medium after aerobic incubation at 30 °C. The method is applicable to: a) products intended for human consumption or for animal feed; b) environmental samples in the area of food and feed production and food handling. ISO 4833-2:2013 is applicable to: 1) products containing heat-sensitive organisms that are likely to form a significant proportion of the total flora (e.g. psychrotrophic organisms in chilled and frozen foods, dried foods, other foods that may contain heat-sensitive organisms); 2) products containing obligately aerobic bacteria that are likely to form a significant proportion of the total flora (e.g. Pseudomonas spp.); 3) products that contain small particles that can prove difficult to distinguish from colonies in a pour plate; 4) products whose intense colour prevents the recognition of colonies in a pour plate; 5) products for which distinction between different types of colony is required as part of the assessment of food quality. In addition to the manual spread plating technique, an annex to ISO 4833-2:2013 also specifies the use of a spiral plater, a rapid method of performing surface colony counts. The applicability of ISO 4833-2:2013 to the examination of certain fermented food and animal feeds is limited and other media or incubation conditions can be more appropriate. However, this method can be applied to such products even though it is possible that the predominant microorganisms in these products are not detected effectively. For some matrices, the method described in ISO 4833-2:2013 can give different results to those obtained using the method described in ISO 4833‑1.  Published 2013-09 Edition : 1 Number of pages : 12 Technical Committee 07.100.30 Food microbiology
ISO 4833-2:2013/Amd 1:2022 Microbiology of the food chain — Horizontal method for the enumeration of microorganisms — Part 2: Colony count at 30 °C by the surface plating technique — Amendment 1: Clarification of scope  Published 2022-01 Edition : 1 Number of pages : 2 Technical Committee 07.100.30 Food microbiology
ISO 4833-2:2013/Cor 1:2014 Microbiology of the food chain — Horizontal method for the enumeration of microorganisms — Part 2: Colony count at 30 °C by the surface plating technique — Technical Corrigendum 1  Published 2014-02 Edition : 1 Number of pages : 1 Technical Committee 07.100.30 Food microbiology
ISO 4833:1978 Microbiology — General guidance for enumeration of micro-organisms — Colony count technique at 30 degrees C  Withdrawn 1978-02 Edition : 1 Number of pages : 4 Technical Committee 07.100.30 Food microbiology
ISO 4833:1991 Microbiology — General guidance for the enumeration of micro-organisms — Colony count technique at 30 degrees C This second edition cancels and replaces the first edition (ISO 4833:1978). It is intended to provide general guidance for the examination of products not dealt with by existing International Standards. These guidelines may not be appropriate for some products in every detail, and for some other products it may be necessary to use different methods. Gives references, and the definition of the term micro-organisms. Specifies principle, culture media and dilution fluid, apparatus and glassware, sampling, preparation of the test sample, procedure, expression of results and test report.  Withdrawn 1991-03 Edition : 2 Number of pages : 5 Technical Committee 07.100.01 Microbiology in general ; 07.100.30 Food microbiology
ISO 6730:1992 Milk — Enumeration of colony-forming units of psychrotrophic micro-organisms — Colony-count technique at 6,5 degrees C Specifies the principle, the diluents and culture medium, the apparatus and glassware, the sampling and procedure, the expression of results, the repeatability and the test report.  Withdrawn 1992-01 Edition : 1 Number of pages : 6 Technical Committee 07.100.30 Food microbiology
ISO 4833:2003 Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of microorganisms — Colony-count technique at 30 degrees C ISO 4833:2003 specifies a horizontal method for the enumeration of microorganisms, by counting the colonies growing in a solid medium after aerobic incubation at 30 degrees Celsius. Subject to some limitations, it is applicable to products intended for human consumption or the feeding of animals. The applicability of ISO 4833:2003 to the examination of certain fermented food and animal feeding stuffs is limited. For the examination of fermented food and animal feeding stuffs, other media and/or incubation conditions might be more appropriate.  Withdrawn 2003-02 Edition : 3 Number of pages : 9 Technical Committee 07.100.30 Food microbiology
ISO 5541-1:1986 Milk and milk products — Enumeration of coliforms — Part 1: Colony count technique at 30 degrees C Specifies a method for enumerating bacteria which, at 30 degrees centigrade, form characteristic colonies and which can ferment lactose with the production of gas under the operational conditions described. The method described is applicable to milk and liquid milk products, dried milk, dried sweet whey, dried buttermilk, lactose, acid casein, lactic casein, rennet casein, caseinate, dried acid whey, cheese and processed cheese, butter, frozen milk products, custard, desserts, and cream. Is to be preferred for samples in which large numbers of coliforms are suspected (more than 100 per gram or 10 per millimetre).  Withdrawn 1986-11 Edition : 1 Number of pages : 7 Technical Committee 07.100.30 Food microbiology
ISO 5541-2:1986 Milk and milk products — Enumeration of coliforms — Part 2: Most probable number technique at 30 degrees C Specifies a method for enumerating bacteria which cause fermentation of lactose with the production of gas and form characteristic growth under the operational conditions describes. Uses a culture technique involving a liquid medium. The calculation of the most probable number is made after incubation at 30 degrees centigrade.  Withdrawn 1986-11 Edition : 1 Number of pages : 8 Technical Committee 07.100.30 Food microbiology
ISO 5552:1979 Meat and meat products — Detection and enumeration of Enterobacteriaceae (Reference methods)  Withdrawn 1979-04 Edition : 1 Number of pages : 6 Technical Committee 07.100.30 Food microbiology
ISO 5552:1997 Meat and meat products — Detection and enumeration of Enterobacteriaceae without resuscitation — MPN technique and colony-count technique  Withdrawn 1997-12 Edition : 2 Number of pages : 15 Technical Committee 07.100.30 Food microbiology
ISO 5944:2001 | IDF 60D: 2001 Milk and milk-based products — Detection of coagulase-positive staphylococci — Most probable number technique  Withdrawn 2001-12 Edition : 1 Number of pages : 14 Technical Committee 07.100.30 Food microbiology
ISO 6391:1988 Meat and meat products — Enumeration of Escherichia coli — Colony count technique at 44 degrees C using membranes  Withdrawn 1988-11 Edition : 1 Number of pages : 5 Technical Committee 07.100.30 Food microbiology
ISO 6391:1997 Meat and meat products — Enumeration of Escherichia coli — Colony-count technique at 44 degrees C using membranes  Withdrawn 1997-12 Edition : 2 Number of pages : 9 Technical Committee 07.100.30 Food microbiology
ISO 6579-1:2017 Microbiology of the food chain — Horizontal method for the detection, enumeration and serotyping of Salmonella — Part 1: Detection of Salmonella spp. ISO 6579-1:2017 specifies a horizontal method for the detection of Salmonella. It is applicable to the following: - products intended for human consumption and the feeding of animals; - environmental samples in the area of food production and food handling; - samples from the primary production stage such as animal faeces, dust, and swabs. With this horizontal method, most of the Salmonella serovars are intended to be detected. For the detection of some specific serovars, additional culture steps may be needed. For Salmonella Typhi and Salmonella Paratyphi, the procedure is described in Annex D. The selective enrichment medium modified semi-solid Rappaport-Vassiliadis (MSRV) agar is intended for the detection of motile Salmonella and is not appropriate for the detection of non-motile Salmonella strains.  Published 2017-02 Edition : 1 Number of pages : 50 Technical Committee 07.100.30 Food microbiology
ISO 6579-1:2017/Amd 1:2020 Microbiology of the food chain — Horizontal method for the detection, enumeration and serotyping of Salmonella — Part 1: Detection of Salmonella spp. — Amendment 1: Broader range of incubation temperatures, amendment to the status of Annex D, and correction of the composition of MSRV and SC  Published 2020-03 Edition : 1 Number of pages : 11 Technical Committee 07.100.30 Food microbiology
ISO 6730:2005 | IDF 101:2005 Milk — Enumeration of colony-forming units of psychrotrophic microorganisms — Colony-count technique at 6,5 degrees C ISO 6730|IDF 101:2005 specifies a method for the enumeration of colony-forming units of psychrotrophic microorganisms in raw and heat-treated milk by means of the colony-count technique at 6,5 degrees Celsius.  Withdrawn 2005-09 Edition : 2 Number of pages : 8 Technical Committee 07.100.30 Food microbiology
ISO 6785:1985 Milk and milk products — Detection of salmonella  Withdrawn 1985-11 Edition : 1 Number of pages : 13 Technical Committee 07.100.30 Food microbiology
ISO/TS 6579-2:2012 Microbiology of food and animal feed — Horizontal method for the detection, enumeration and serotyping of Salmonella — Part 2: Enumeration by a miniaturized most probable number technique ISO/TS 6579-2 gives a method for the enumeration of Salmonella spp. present in: products intended for human consumption and for the feeding of animals; environmental samples in the area of food production and food handling; animal faeces; and environmental samples from the primary production stage by calculation of the most probable number (MPN). The method is based on miniaturization of the dilution, pre-enrichment and selective enrichment steps. The selective enrichment medium, modified semi-solid Rappaport?Vassiliadis (MSRV), is intended for the detection of motile salmonellae and is not appropriate for the detection of non-motile salmonellae. It is possible that the method is less appropriate to enumerate Salmonella ser. Typhi and Salmonella ser. Paratyphi. The method is not appropriate for the enumeration of Salmonella spp. in (very) low contaminated samples (<1 cfu/g).  Published 2012-11 Edition : 1 Number of pages : 18 Technical Committee 07.100.30 Food microbiology
ISO/TR 6579-3:2014 Microbiology of the food chain — Horizontal method for the detection, enumeration and serotyping of Salmonella — Part 3: Guidelines for serotyping of Salmonella spp. ISO/TR 6579-3:2014 gives guidance on the procedure for serotyping Salmonella serovars and is applicable to the serotyping of pure cultures of Salmonella spp., independent of the source from which they are isolated.  Published 2014-07 Edition : 1 Number of pages : 33 Technical Committee 07.100.30 Food microbiology
ISO/CD TS 6579-4 Microbiology of the food chain — Horizontal method for the detection, enumeration and serotyping of Salmonella — Part 4: Identification of monophasic Salmonella Typhimurium (1,4,[5],12:i:-) by polymerase chain reaction (PCR)  Under development Edition : 1 Technical Committee 07.100.30 Food microbiology
ISO 6579:1981 Microbiology — General guidance on methods for the detection of Salmonella  Withdrawn 1981-03 Edition : 1 Number of pages : 15 Technical Committee 07.100.30 Food microbiology
ISO 6579:1993 Microbiology — General guidance on methods for the detection of Salmonella The methods proposed necessitate four successive stages: pre-enrichment in non-selective liquid medium, enrichment in selective liquid media, plating out and recognition, confirmation. Annex A gives a diagram of the procedure, annex B describes composition and preparation of culture media and reagents, annex C specifies brilliant green.  Withdrawn 1993-09 Edition : 3 Number of pages : 16 Technical Committee 07.100.30 Food microbiology
ISO 6579:2002 Microbiology of food and animal feeding stuffs — Horizontal method for the detection of Salmonella spp. ISO 6579:2002 specifies a horizontal method for the detection of Salmonella, including Salmonella Typhi and Salmonella Paratyphi. It is applicable to products intended for human consumption and the feeding of animals, and to environmental samples in the area of food production and food handling.  Withdrawn 2002-07 Edition : 4 Number of pages : 27 Technical Committee 07.100.30 Food microbiology