| Name |
Description |
Abstract |
Status |
Publication date |
Edition |
Number of pages |
Technical committee |
ICS |
| ISO 14461-2:2005 | IDF 169-2:2005 |
Milk and milk products — Quality control in microbiological laboratories — Part 2: Determination of the reliability of colony counts of parallel plates and subsequent dilution steps |
ISO 14461-2|IDF 169-2:2005 describes a routine procedure for the evaluation of results of the enumeration of microorganisms using colony-count methods with subsequent 10-fold dilution steps and one plate or two parallel plates within each dilution step.
This routine procedure is applied regularly in each laboratory performing colony counts. It provides criteria for the acceptability of differences between the results from parallel plates and subsequent dilution steps, as follows.
The results (colony counts) obtained from parallel plates are compared with tabulated limits for given colony counts. If these limits are exceeded, a technical problem when performing the parallel determinations may be indicated.
The results (sums of colony counts) of two parallel plates of two subsequent 10-fold dilution steps are compared with tabulated limits for given sums of colony counts. If these limits are exceeded, a technical problem when performing the dilutions may be indicated.
If the limits mentioned above are exceeded in more cases than expected, this indicates that the test procedure lacks reliability.
|
Published |
2005-04 |
Edition : 1 |
Number of pages : 18 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 15174:2002 | IDF 176: 2002 |
Milk and milk products — Microbial coagulants — Determination of total milk-clotting activity |
ISO 15174|IDF 176:2002 describes a method to compare the total milk-clotting activity of a microbial coagulant sample with the milk-clotting activity of an international microbial coagulant reference standard on a standard milk substrate prepared with a calcium chloride solution containing 0,5 g/l of calcium chloride (pH approximately 6,5).
|
Withdrawn |
2002-09 |
Edition : 1 |
Number of pages : 8 |
Technical Committee |
07.100.30
Food microbiology
;
67.100.10
Milk and processed milk products
|
| ISO 15174:2012 | IDF 176:2012 |
Milk and milk products — Microbial coagulants — Determination of total milk-clotting activity |
ISO 15174:2012 specifies a method for comparison of the total milk-clotting activity of a microbial coagulant sample with the milk-clotting activity of an international microbial coagulant reference standard on a standard milk substrate prepared with a calcium chloride solution of concentration 0,5 g/l (pH ~6,5).
|
Published |
2012-05 |
Edition : 2 |
Number of pages : 9 |
Technical Committee |
07.100.30
Food microbiology
;
67.100.10
Milk and processed milk products
|
| ISO 15213-1:2023 |
Microbiology of the food chain — Horizontal method for the detection and enumeration of Clostridium spp. — Part 1: Enumeration of sulfite-reducing Clostridium spp. by colony-count technique |
This document specifies the enumeration of sulfite-reducing Clostridium spp. by the colony-count technique.
This document is applicable to:
— products intended for human consumption;
— products for feeding animals;
— environmental samples in the area of food and feed production and handling;
— samples from the primary production stage.
NOTE This method has been validated in an interlaboratory study for the following food categories:
— ready-to-eat, ready-to-reheat meat products;
— eggs and egg products (derivates);
— processed fruits and vegetables;
— infant formula and infant cereals;
— multi-component foods or meal components.
It has also been validated for the following other categories:
— pet food and animal feed;
— environmental samples (food or feed production).
As this method has been validated for at least five food categories, this method is applicable for a broad range of food. For detailed information on the validation, see Clause 11 and Annex C. Since the method is not commonly used for samples in the primary production stage, this category was not included in the interlaboratory study. Therefore, no performance characteristics were obtained for this category.
This horizontal method was originally developed for the examination of all samples belonging to the food chain. Based on the information available at the time of publication of this document, this method is considered to be fully suited to the examination of all samples belonging to the food chain. However, because of the large variety of products in the food chain, it is possible that this horizontal method is not appropriate in every detail for all products. Nevertheless, it is expected that the required modifications are minimized so that they do not result in a significant deviation from this horizontal method.
This technique is suitable for, but not limited to, the enumeration of microorganisms in test samples with a minimum of 10 colonies counted on a plate. This corresponds to a level of contamination that is expected to be higher than 10 cfu/ml for liquid samples or higher than 100 cfu/g for solid samples.
|
Published |
2023-01 |
Edition : 1 |
Number of pages : 21 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/DIS 15213-2 |
Microbiology of the food chain — Horizontal method for the detection and enumeration of Clostridium spp. — Part 2: Enumeration of Clostridium perfringens by colony-count technique |
|
Under development |
|
Edition : 1 |
Number of pages : 42 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/CD TS 15213-3 |
Microbiology of the food chain — Horizontal method for the detection and enumeration of Clostridium spp. — Part 3: Detection of Clostridium perfringens |
|
Under development |
|
Edition : 1 |
|
Technical Committee |
07.100.30
Food microbiology
|
| ISO 15213:2003 |
Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of sulfite-reducing bacteria growing under anaerobic conditions |
ISO 15213:2003 specifies a horizontal method for the enumeration of sulfite-reducing bacteria growing under anaerobic conditions. It is applicable to
products intended for human consumption and the feeding of animals, andenvironmental samples in the area of food production and food handling.
|
Withdrawn |
2003-05 |
Edition : 1 |
Number of pages : 6 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 15216-1:2017 |
Microbiology of the food chain — Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR — Part 1: Method for quantification |
ISO 15216-1:2017 specifies a method for the quantification of levels of HAV and norovirus genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs (soft fruit, leaf, stem and bulb vegetables, bottled water, BMS) or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR.
This method is not validated for detection of the target viruses in other foodstuffs (including multi-component foodstuffs), or any other matrices, nor for the detection of other viruses in foodstuffs, food surfaces or other matrices.
|
Published |
2017-03 |
Edition : 1 |
Number of pages : 48 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/TS 15216-1:2013 |
Microbiology of food and animal feed — Horizontal method for determination of hepatitis A virus and norovirus in food using real-time RT-PCR — Part 1: Method for quantification |
ISO/TS 15216-1:2013 describes a method for quantification of levels of HAV and NoV genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR.
This approach is also relevant for detection of the target viruses on fomites, or of other human viruses in foodstuffs, on food surfaces or on fomites following appropriate validation and using target-specific primer and probe sets.
|
Withdrawn |
2013-03 |
Edition : 1 |
Number of pages : 31 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 15216-1:2017/Amd 1:2021 |
Microbiology of the food chain — Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR — Part 1: Method for quantification — Amendment 1 |
|
Published |
2021-03 |
Edition : 1 |
Number of pages : 3 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 15216-2:2019 |
Microbiology of the food chain — Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR — Part 2: Method for detection |
This document specifies a method for detection of hepatitis A virus (HAV) and norovirus genogroups I (GI) and II (GII), from test samples of foodstuffs [(soft fruit, leaf, stem and bulb vegetables, bottled water, bivalve molluscan shellfish (BMS)] or surfaces using real-time RT-PCR.
This method is not validated for detection of the target viruses in other foodstuffs (including multi-component foodstuffs), or any other matrices, nor for the detection of other viruses in foodstuffs, surfaces or other matrices.
|
Published |
2019-07 |
Edition : 1 |
Number of pages : 40 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/TS 15216-2:2013 |
Microbiology of food and animal feed — Horizontal method for determination of hepatitis A virus and norovirus in food using real-time RT-PCR — Part 2: Method for qualitative detection |
ISO/TS 15216-2:2013 describes a method for qualitative detection of HAV and NoV genogroups I (GI) and II (GII), from test samples of foodstuffs or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR.
This approach is also relevant for detection of the target viruses on fomites, or of other human viruses in foodstuffs, on food surfaces or on fomites following appropriate validation and using target-specific primer and probe sets.
|
Withdrawn |
2013-03 |
Edition : 1 |
Number of pages : 27 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 16140-1:2016 |
Microbiology of the food chain — Method validation — Part 1: Vocabulary |
ISO 16140-1:2016 defines general terms and definitions relating to method validation of microbiology in the food chain.
It is applicable to the validation of methods for the analysis (detection or quantification) of microorganisms in
- products intended for human consumption,
- products intended for animal feeding,
- environmental samples in the area of food and feed production, handling, and
- samples from the primary production stage.
|
Published |
2016-06 |
Edition : 1 |
Number of pages : 12 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 16140-2:2016 |
Microbiology of the food chain — Method validation — Part 2: Protocol for the validation of alternative (proprietary) methods against a reference method |
ISO 16140-2:2016 specifies the general principle and the technical protocol for the validation of alternative, mostly proprietary, methods for microbiology in the food chain. Validation studies according to ISO 16140-2:2016 are intended to be performed by organizations involved in method validation.
It is applicable to the validation of methods for the analysis (detection or quantification) of microorganisms in
- products intended for human consumption,
- products intended for animal feeding,
- environmental samples in the area of food and feed production, handling, and
- samples from the primary production stage.
It is in particular applicable to bacteria and fungi. Some clauses of ISO 16140-2:2016 could be applicable to other (micro) organisms or their metabolites on a case-by-case-basis. In the future, guidance for other organisms (e.g. viruses and parasites) will be included in ISO 16140:2016 (all parts).
|
Published |
2016-06 |
Edition : 1 |
Number of pages : 66 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 16140-2:2016/CD Amd 1 |
Microbiology of the food chain — Method validation — Part 2: Protocol for the validation of alternative (proprietary) methods against a reference method — Amendment 1 |
|
Under development |
|
Edition : 1 |
|
Technical Committee |
07.100.30
Food microbiology
|
| ISO 16140-3:2021 |
Microbiology of the food chain — Method validation — Part 3: Protocol for the verification of reference methods and validated alternative methods in a single laboratory |
This document specifies the protocol for the verification of reference methods and validated alternative methods for implementation in the user laboratory.
This document is applicable to the verification of methods used for the analysis (detection and/or quantification), confirmation and typing of microorganisms in:
— products intended for human consumption;
— products intended for animal feeding;
— environmental samples in the area of food and feed production, handling;
— samples from the primary production stage.
This document is, in particular, applicable to bacteria and fungi. Some clauses can be applicable to other (micro)organisms or their metabolites, to be determined on a case-by-case basis.
The technical protocols for the verification of validated qualitative methods and validated quantitative methods are described in Clauses 5 and 6. The technical protocol for the verification of validated alternative confirmation and typing methods is described in Clause 7. The protocols for the verification of non-validated reference methods are described in Annex F.
|
Published |
2021-01 |
Edition : 1 |
Number of pages : 70 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 16140-4:2020 |
Microbiology of the food chain — Method validation — Part 4: Protocol for method validation in a single laboratory |
This document specifies the general principles and the technical protocols for single-laboratory validation of methods for microbiology in the food chain. The protocols in this document only validate the method for the laboratory conducting the study.
This document is applicable to single-laboratory validation of:
— methods used in the analysis (detection or quantification) of microorganisms in:
— products intended for human consumption;
— products intended for animal feeding;
— environmental samples in the area of food and feed production, handling;
— samples from the primary production stage;
— methods for the confirmation or typing of microorganisms. This validation will replace only the confirmation or typing procedure of a specified method (see Annex G).
This document is, in particular, applicable to bacteria and fungi. Some clauses can be applicable to other (micro)organisms or their metabolites, to be determined on a case-by-case basis.
Single-laboratory validation is required if an interlaboratory validation in accordance with ISO 16140-2 is not appropriate. Possible applications are:
— validation of an in-house method;
— method evaluation study in the validation process of a reference method in accordance with ISO 17468;
— extension of the scope of an ISO 16140-2 validated method, e.g. category extension or test portion size;
— modifications of existing methods.
Single-laboratory validation is the second step in the standardization of a reference method (see ISO 17468). It is only applicable to methods that are fully specified with regard to all relevant parameters (including tolerances on temperatures and specifications on culture media) and that have already been optimized.
|
Published |
2020-07 |
Edition : 1 |
Number of pages : 46 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 16140-4:2020/CD Amd 1 |
Microbiology of the food chain — Method validation — Part 4: Protocol for method validation in a single laboratory — Amendment 1: Annex H - Validation of larger test portion size for qualitative methods |
|
Under development |
|
Edition : 1 |
|
Technical Committee |
07.100.30
Food microbiology
|
| ISO 16140-5:2020 |
Microbiology of the food chain — Method validation — Part 5: Protocol for factorial interlaboratory validation for non-proprietary methods |
This document specifies the general principles and the technical protocols (based on orthogonal, factorial studies) for the validation of non-proprietary methods for microbiology of the food chain.
This document is applicable to the validation of methods used for the analysis (detection or quantification) of microorganisms in:
— products intended for human consumption;
— products intended for animal feeding;
— environmental samples in the area of food and feed production, handling;
— samples from the primary production stage.
This document is, in particular, applicable to bacteria and fungi. Some clauses can be applicable to other (micro)organisms or their metabolites, to be determined on a case-by-case basis.
This document specifies protocols for the validation against a reference method for both quantitative and qualitative methods. This document also provides a protocol for the validation of quantitative methods without a reference method. Qualitative methods cannot be validated without a reference method in accordance with this document.
NOTE ISO 16140-2 specifies the general principle and the technical protocol for the validation of alternative, mostly proprietary, methods against a reference method.
This document is only applicable to the validation of methods that are fully specified with regard to all relevant parameters (including tolerances on temperatures and specifications on culture media) and that have already been optimized.
Methods that have been validated in accordance with this document can be used by the laboratories of the specified population of laboratories.
|
Published |
2020-07 |
Edition : 1 |
Number of pages : 34 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 16140-6:2019 |
Microbiology of the food chain — Method validation — Part 6: Protocol for the validation of alternative (proprietary) methods for microbiological confirmation and typing procedures |
This document specifies the general principle and the technical protocol for the validation of alternative confirmation methods for microbiology in the food chain. This document compares the result of the alternative confirmation method against the confirmation procedure of a reference method or, if needed, a reference confirmation method (e.g. whole genome sequencing).
This document is applicable to the validation of alternative confirmation methods used for the analysis (detection or quantification) of isolated microorganisms in:
— products intended for human consumption;
— products intended for animal feeding;
— environmental samples in the area of food and feed production, handling;
— samples from the primary production stage.
Validated alternative confirmation methods can be used to replace (partly or completely) the confirmation procedure described in:
— the reference method;
— an alternative method validated in accordance with ISO 16140-2 only if one of the isolation agars specified in the validation study of the alternative confirmation method is used.
This document is also applicable to the validation of alternative typing methods, where the reference method can be, for example, a serological method (e.g. serotyping of Salmonella) or a molecular method (e.g. typing of Shiga toxin-producing E. coli).
This document is, in particular, applicable to bacteria and fungi. Some clauses can be applicable to other (micro)organisms, to be determined on a case-by-case basis.
Validation studies in accordance with this document are primarily intended to be performed by organizations or expert laboratories involved in method validation, but can also be used by a single laboratory, especially when performing in-house validation under certain conditions (see ISO 16140-4).
|
Published |
2019-11 |
Edition : 1 |
Number of pages : 25 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/TS 16649-3:2005 |
Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli — Part 3: Most probable number technique using 5-bromo-4-chloro-3-indolyl-beta-D-glucuronide |
ISO/TS 16649-3:2005 specifies a horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli, by means of the liquid-medium culture technique and calculation of the most probable number (MPN) after incubation at 37 °C, then at 44 °C.
This Technical Specification is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling.
The method is suitable for the enumeration of cells of Escherichia coli that may have been subjected to stress arising from dehydration, freezing, exposure to a saline (such as marine) environment or damage by disinfectants such as chlorine-containing products.
|
Withdrawn |
2005-09 |
Edition : 1 |
Number of pages : 8 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/DIS 16140-7 |
Microbiology of the food chain — Method validation — Part 7: Protocol for the validation of identification methods of microorganisms |
This document specifies the general principle and the technical protocol for the validation of identification methods of microorganisms for microbiology in the food chain. As there is no reference method, this document provides a protocol to evaluate the performance characteristics and validate the method workflow using well-defined strains. When required, an additional identification method can be used.
This document is applicable to the validation of identification methods of microorganisms that are used for the analysis of microorganisms in:
— products intended for human consumption;
— products intended for animal feeding;
— environmental samples in the area of food and feed production, handling;
— samples from the primary production stage.
Validated identification methods cannot be used instead of confirmation described in:
— the reference method;
— an alternative method validated in accordance with ISO 16140-2;
— an alternative method validated in accordance with ISO 16140-6.
In these instances, the identification method shall be validated in accordance with the ISO 16140-6 to be used as a confirmation method.
This document is, in particular, applicable to bacteria and fungi. Some clauses can be applicable to other (micro)organisms, to be determined on a case-by-case basis.
|
Under development |
|
Edition : 1 |
Number of pages : 33 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 16140:2003 |
Microbiology of food and animal feeding stuffs — Protocol for the validation of alternative methods |
ISO 16140:2003 defines the general principle and the technical protocol for the validation of alternative methods in the field of microbiological analysis of food, animal feeding stuffs and environmental and veterinary samples for the validation of alternative methods which can be used in particular in the framework of the official control, and the international acceptance of the results obtained by the alternative method.
It also establishes the general principles of certification of these alternative methods, based on the validation protocol defined in ISO 16140:2003.
Where an alternative method is used on a routine basis for internal laboratory use without the requirement to meet (higher) external criteria of quality assurance, a less stringent comparative validation of the alternative method than that set in ISO 16140:2003 may be appropriate.
|
Withdrawn |
2003-05 |
Edition : 1 |
Number of pages : 74 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 16140:2003/Amd 1:2011 |
Microbiology of food and animal feeding stuffs — Protocol for the validation of alternative methods — Amendment 1 |
|
Withdrawn |
2011-08 |
Edition : 1 |
Number of pages : 18 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 16649-1:2001 |
Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli — Part 1: Colony-count technique at 44 degrees C using membranes and 5-bromo-4-chloro-3-indolyl beta-D-glucuronide |
|
Withdrawn |
2001-04 |
Edition : 1 |
Number of pages : 9 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 16649-1:2018 |
Microbiology of the food chain — Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli — Part 1: Colony-count technique at 44 degrees C using membranes and 5-bromo-4-chloro-3-indolyl beta-D-glucuronide |
ISO 16649-1:2018 specifies a horizontal method for the enumeration of β-glucuronidase-positive Escherichia coli by colony-count technique after resuscitation using membranes and incubation at 44 °C on a solid medium containing a chromogenic ingredient for detection of the enzyme β-glucuronidase[9][10][13][14][17][18][19][20]. It is applicable to
- products intended for human consumption,
- products intended for feeding animals,
- environmental samples in the area of food production and food handling, and
- samples from the primary production stage such as animal faeces, dust, and swabs.
|
Published |
2018-04 |
Edition : 2 |
Number of pages : 11 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 16649-2:2001 |
Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli — Part 2: Colony-count technique at 44 degrees C using 5-bromo-4-chloro-3-indolyl beta-D-glucuronide |
|
Published |
2001-04 |
Edition : 1 |
Number of pages : 8 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 16649-3:2015 |
Microbiology of the food chain — Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli — Part 3: Detection and most probable number technique using 5-bromo-4-chloro-3-indolyl-ß-D-glucuronide |
ISO 16649-3:2015 specifies a horizontal method for the detection and enumeration of β-glucuronidase positive Escherichia coli, by means of the liquid-medium culture technique and calculation of the most probable number (MPN) after incubation at (37 ± 1) °C, then at (44 ± 1) °C. This part of ISO 16649 is applicable to the following:
- products intended for human consumption and the feeding of animals;
- environmental samples in the area of food production and food handling.
The method is suitable for the enumeration of cells of E. coli that might have been subjected to stress arising from dehydration, freezing, and exposure to a saline (such as marine) environment or damage by disinfectants such as chlorine-containing products.
A limitation of the applicability of this part of ISO 16649 is imposed by the susceptibility of the method to a large degree of variability. The method is intended to be applied and the results interpreted in the light of the information given in Clause 11.
This method has not been fully evaluated for all matrices (e.g. for milk and milk products). ISO 7251 is intended to be used for milk and milk products.
|
Published |
2015-05 |
Edition : 1 |
Number of pages : 10 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 16654:2001 |
Microbiology of food and animal feeding stuffs — Horizontal method for the detection of Escherichia coli O157 |
|
Published |
2001-05 |
Edition : 1 |
Number of pages : 13 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 16654:2001/Amd 1:2017 |
Microbiology of food and animal feeding stuffs — Horizontal method for the detection of Escherichia coli O157 — Amendment 1: Annex B: Result of interlaboratory studies |
|
Published |
2017-03 |
Edition : 1 |
Number of pages : 4 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 16654:2001/Amd 2:2023 |
Microbiology of food and animal feeding stuffs — Horizontal method for the detection of Escherichia coli O157 — Amendment 2: Inclusion of performance testing of all culture media and reagents |
|
Published |
2023-01 |
Edition : 1 |
Number of pages : 2 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 17410:2001 |
Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of psychrotrophic microorganisms |
|
Withdrawn |
2001-05 |
Edition : 1 |
Number of pages : 7 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 17410:2019 |
Microbiology of the food chain — Horizontal method for the enumeration of psychrotrophic microorganisms |
This document specifies a horizontal method for the enumeration of psychrotrophic microorganisms that are able to grow and form colonies on a solid agar culture medium after aerobic incubation at 6,5 °C.
This document is applicable to
— products intended for human consumption,
— products intended for animal feeding,
— environmental samples in the area of food and feed production, handling, and
— samples from the primary production stage.
NOTE Annex B specifies a rapid method for the estimated enumeration of psychrotrophic microorganisms in raw and pasteurized milk.
|
Published |
2019-07 |
Edition : 2 |
Number of pages : 10 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 17468:2016 |
Microbiology of the food chain — Technical requirements and guidance on establishment or revision of a standardized reference method |
ISO 17468:2016 gives technical requirements and guidance on the establishment or revision of standardized reference methods for the analysis (detection or quantification) of microorganisms in
- products intended for human consumption and for the feeding of animals,
- environmental samples in the area of food/feed production and food/feed handling, and
- samples from the primary production stage.
It defines the technical stage (or early stage) of the establishment of a new standardized reference method or of the revision of an existing standardized reference method. It includes, in particular, requirements and guidance on the validation of the selected method.
It is intended to be implemented in particular by ISO/TC 34/SC 9 and its corresponding structure at CEN level, CEN/TC 275, Food analysis - Horizontal methods, Working Group 6, Microbiology of the food chain.
|
Published |
2016-06 |
Edition : 1 |
Number of pages : 5 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/DIS 17468 |
Microbiology of the food chain — Technical requirements and guidance on establishment or revision of a standardized reference method |
|
Under development |
|
Edition : 2 |
Number of pages : 10 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 17604:2003 |
Microbiology of food and animal feeding stuffs — Carcass sampling for microbiological analysis |
ISO 17604:2003 specifies sampling methods for the detection and enumeration of microorganisms on the carcass surface of freshly slaughtered (red) meat animals. The microbiological sampling can be carried out as part of the process control (and to verify process control) in slaughter establishments for cattle, horses, pigs, sheep, goats and game raised in captivity, risk-based assurance systems for product safety, and surveillance programmes for the prevalence of pathogenic microorganisms.
ISO 17604:2003 includes the use of destructive and non-destructive techniques depending on the reason for the sample collection.
It does not consider the use of sampling plans.
|
Withdrawn |
2003-09 |
Edition : 1 |
Number of pages : 14 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 17604:2003/Amd 1:2009 |
Microbiology of food and animal feeding stuffs — Carcass sampling for microbiological analysis — Amendment 1: Sampling of poultry carcasses |
|
Withdrawn |
2009-06 |
Edition : 1 |
Number of pages : 3 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 17604:2015 |
Microbiology of the food chain — Carcass sampling for microbiological analysis |
ISO 17604:2015 specifies sampling methods for the detection and enumeration of microorganisms on the surface of carcasses or parts of carcasses of slaughtered meat animals. The microbiological sampling can be carried out as part of
- process hygiene control (to validate and or verify process control, e.g. total counts and Enterobacteriaceae) in slaughter establishments for large mammals, poultry, and game,
- risk-based assurance systems for product safety, and
- monitoring or surveillance programmes for the prevalence and/or numbers of pathogenic microorganisms.
This International Standard includes the use of excision and swabbing techniques depending on the reason for sample collection. It also includes the use of carcass rinsing for the examination of carcasses of poultry and some small animals. Annex A shows sampling sites on the carcasses of various animal species.
|
Published |
2015-08 |
Edition : 2 |
Number of pages : 13 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/TS 17728:2015 |
Microbiology of the food chain — Sampling techniques for microbiological analysis of food and feed samples |
ISO/TS 17728:2015 applies to the collection of samples before submission to the laboratory for microbiological examination. It provides general instructions and specific requirements for obtaining samples and for transport to the laboratory.
Sampling plans are not included in the scope of this Technical Specification.
|
Published |
2015-06 |
Edition : 1 |
Number of pages : 17 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/TS 17919:2013 |
Microbiology of the food chain — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — Detection of botulinum type A, B, E and F neurotoxin-producing clostridia |
ISO/TS 17919:2013 specifies a horizontal method for the molecular detection of clostridia carrying botulinum neurotoxin A, B, E, and F genes by a PCR method. This method detects the genes and not the toxins, therefore a positive result does not necessarily mean the presence of these toxins in the sample investigated. ISO/TS 17919:2013 is applicable to products for human consumption, animal feed, and environmental samples.
The PCR assays for detection of genetic sequences encoding specific toxin types are described in annexes.
|
Published |
2013-11 |
Edition : 1 |
Number of pages : 47 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 18465:2017 |
Microbiology of the food chain — Quantitative determination of emetic toxin (cereulide) using LC-MS/MS |
ISO 18465:2017 describes the quantitative analysis of the emetic toxin cereulide using high performance liquid chromatography (HPLC) or ultra performance liquid chromatography (UHPLC) connected to a tandem mass spectrometer (LC-MS/MS).
ISO 18465:2017 is applicable to the analysis of the toxin in products intended for human consumption.
|
Published |
2017-01 |
Edition : 1 |
Number of pages : 14 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 18593:2004 |
Microbiology of food and animal feeding stuffs — Horizontal methods for sampling techniques from surfaces using contact plates and swabs |
ISO 18593:2004 specifies horizontal methods for sampling techniques using contact plates or swabs on surfaces in the food industry environment (and food processing plants), with a view of detecting or enumerating viable microorganisms.
|
Withdrawn |
2004-06 |
Edition : 1 |
Number of pages : 8 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 18593:2018 |
Microbiology of the food chain — Horizontal methods for surface sampling |
This document specifies horizontal methods for sampling techniques using contact plates, stick swabs, sponges and cloths on surfaces in the food chain environment in order to detect and enumerate culturable microorganisms such as pathogenic or non-pathogenic bacteria or yeasts and moulds.
NOTE The term "environment" means any item in contact with the food product or likely to represent a contamination or recontamination source; for example, material, premises or operators.
This document does not apply to the validation of cleaning and disinfection procedures.
This document does not apply to sampling techniques for primary production samples, which are covered by ISO 13307. Sampling techniques for carcasses are covered by ISO 17604. Sampling techniques for analysis of noroviruses and hepatitis A viruses are covered by ISO 15216-1.
This document does not give advice on sampling frequency, the number of sampling points, or the need to rotate sampling points, as these are chosen on a case-by-case basis.
|
Published |
2018-06 |
Edition : 2 |
Number of pages : 11 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 18743:2015 |
Microbiology of the food chain — Detection of Trichinella larvae in meat by artificial digestion method |
ISO 18743:2015 specifies a method of detection of Trichinella spp. muscle stage larvae in meat of individual animal carcasses intended for human consumption. It is applicable to the examination of meat from domestic and sylvatic animal species, which can be infected by nematodes of the genus Trichinella.
This method does not allow the identification of the species or genotype of detected parasites; species or genotype identification can be carried out by molecular methods.
The method described in this International Standard is intended to be used in conjunction with the guidelines in the OIE Manual of Diagnostic Tests and Vaccines and by the International Commission on Trichinellosis (ICT) for Trichinella testing and the inspection of carcasses intended for human consumption, unless it has been demonstrated by other means that the animal was not at risk for exposure to Trichinella.
The artificial digestion/magnetic stirrer method is considered to be the standard method because it has proven to give the most reliable results in validation studies.
NOTE Provided equivalence with the method described within this International Standard can be documented, alternative methods can be used for analysis.
|
Published |
2015-09 |
Edition : 1 |
Number of pages : 15 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 18743:2015/FDAmd 1 |
Microbiology of the food chain — Detection of Trichinella larvae in meat by artificial digestion method — Amendment 1: Method validation studies and performance characteristics |
|
Under development |
|
Edition : 1 |
Number of pages : 6 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 18744:2016 |
Microbiology of the food chain — Detection and enumeration of Cryptosporidium and Giardia in fresh leafy green vegetables and berry fruits |
ISO 18744:2016 specifies a method that is applicable for the detection and enumeration of Cryptosporidium oocysts and Giardia cysts on or in food products that are described herein as fresh leafy green vegetables and berry fruits. With suitable controls, it may also be applicable for the examination of other fresh produce.
The microscopy descriptions are for Cryptosporidium spp. oocysts and Giardia duodenalis cysts of size ranges which include those species (Cryptosporidium) or assemblages (Giardia) known to be pathogenic to humans.
This method does not include any molecular analysis and therefore is not suitable for the determination of the species or genotypes/assemblages of Cryptosporidium oocysts and Giardia cysts. The method will detect all species and genotypes/assemblages that are known to be pathogenic for humans and also others that are not. For further identification, molecular typing assays are required. However, these cannot be reliably performed if process positive controls have been spiked into the samples, as the result of molecular typing assays will be obfuscated.
This method does not allow the determination of viability or infectivity of any Cryptosporidium oocysts and Giardia cysts which may be present.
|
Published |
2016-04 |
Edition : 1 |
Number of pages : 21 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/TS 18867:2015 |
Microbiology of the food chain — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — Detection of pathogenic Yersinia enterocolitica and Yersinia pseudotuberculosis |
ISO/TS 18867:2015 specifies two horizontal methods for detection of the pathogenic bioserotypes of Y. enterocolitica and one for detection of Y. pseudotuberculosis by using real-time PCR-based methods. The described methods allow for the detection of the two pathogens in enrichments and allow the isolation of colonies. Y. pestis, the causative agent of bubonic and pneumonic plague harbours a variant of the ail gene as well and will be detected by the same primer/probe set as Y. pseudotuberculosis. However, Y. pestis is normally not associated with food. This Technical Specification is applicable to products for human consumption, animal feeding stuffs, and environmental samples.
|
Published |
2015-09 |
Edition : 1 |
Number of pages : 30 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 19020:2017 |
Microbiology of the food chain — Horizontal method for the immunoenzymatic detection of staphylococcal enterotoxins in foodstuffs |
ISO 19020:2017 specifies a screening method for the detection of staphylococcal enterotoxins SEA, SEB, SECs, SED and SEE in foodstuffs. It consists of two main steps: a) extraction followed by a concentration based on dialysis principle; and b) an immunoenzymatic detection using commercially available detection kits.
ISO 19020:2017 is applicable to the screening of staphylococcal enterotoxins SEA to SEE in products intended for human consumption.
Other staphylococcal enterotoxins such as types SEG, SEH, SEI, SER, SES and SET can also cause illness. Due to the lack of commercially available detection kits, ISO 19020:2017 is applicable only to types SEA to SEE, but may apply to other types of toxins, subject to validation of the method.
|
Published |
2017-06 |
Edition : 1 |
Number of pages : 22 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 19036:2019 |
Microbiology of the food chain — Estimation of measurement uncertainty for quantitative determinations |
This document specifies requirements and gives guidance for the estimation and expression of measurement uncertainty (MU) associated with quantitative results in microbiology of the food chain.
It is applicable to the quantitative analysis of:
— products intended for human consumption or the feeding of animals;
— environmental samples in the area of food production and food handling;
— samples at the stage of primary production.
The quantitative analysis is typically carried out by enumeration of microorganisms using a colony-count technique. This document is also generally applicable to other quantitative analyses, including:
— most probable number (MPN) techniques;
— instrumental methods, such as impediometry, adenosine triphosphate (ATP) and flow cytometry;
— molecular methods, such as methods based on quantitative polymerase chain reaction (qPCR).
The uncertainty estimated by this document does not include systematic effects (bias).
|
Published |
2019-10 |
Edition : 1 |
Number of pages : 38 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/TS 19036:2006 |
Microbiology of food and animal feeding stuffs — Guidelines for the estimation of measurement uncertainty for quantitative determinations |
ISO/TS 19036:2006 gives guidance for the estimation and expression of measurement uncertainty (MU) associated with quantitative results in food microbiology.
It is applicable to the quantitative analysis of products intended for human consumption and the feeding of animals, and of environmental samples in the area of food production and food handling, typically carried out by enumeration of microorganisms using a colony-count technique, but applicable also to quantitative analysis by alternative instrumental methods.
ISO/TS 19036:2006 is not applicable to enumeration using a most probable number technique, or the analysis of low levels of microorganisms.
|
Withdrawn |
2006-02 |
Edition : 1 |
Number of pages : 17 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/TS 19036:2006/Amd 1:2009 |
Microbiology of food and animal feeding stuffs — Guidelines for the estimation of measurement uncertainty for quantitative determinations — Amendment 1: Measurement uncertainty for low counts |
|
Withdrawn |
2009-02 |
Edition : 1 |
Number of pages : 7 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 19343:2017 |
Microbiology of the food chain — Detection and quantification of histamine in fish and fishery products — HPLC method |
ISO 19343:2017 specifies a high performance liquid chromatography (HPLC) method to analyse histamine in fish and fishery products (fish sauces, fish maturated by enzyme in brine, etc.) intended for human consumption.
|
Published |
2017-06 |
Edition : 1 |
Number of pages : 14 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 20128:2006 | IDF 192:2006 |
Milk products — Enumeration of presumptive Lactobacillus acidophilus on a selective medium — Colony-count technique at 37 degrees C |
ISO 20128|IDF 192:2006 specifies a method for the enumeration of presumptive Lactobacillus acidophilus in milk products on a selective medium by using a colony-count technique at 37 °C.
The method is applicable to fermented and non-fermented milks, milk powders and infant formulae where presumptive L. acidophilus is present and in combination with other lactic acid bacteria and bifidobacteria.
The method is not applicable when the number of presumptive L. acidophilus is less than 104 CFU/g and the numbers of Lactobacillus rhamnosus, Lactobacillus reuteri and Lactobacillus paracasei subsp. paracasei are greater than 106 CFU/g.
|
Published |
2006-05 |
Edition : 1 |
Number of pages : 11 |
Technical Committee |
07.100.30
Food microbiology
;
67.100.10
Milk and processed milk products
|
| ISO/TS 21872-1:2007/Cor 1:2008 |
Microbiology of food and animal feeding stuffs — Horizontal method for the detection of potentially enteropathogenic Vibrio spp. — Part 1: Detection of Vibrio parahaemolyticus and Vibrio cholerae — Technical Corrigendum 1 |
|
Withdrawn |
2008-03 |
Edition : 1 |
Number of pages : 1 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 20836:2021 |
Microbiology of the food chain — Polymerase chain reaction (PCR) for the detection of microorganisms — Thermal performance testing of thermal cyclers |
This document specifies requirements for the installation, maintenance, temperature calibration and temperature performance testing of standard thermal cyclers and real-time thermal cyclers. It is applicable to the detection of microorganisms as well as any other applications in the food chain using polymerase chain reaction (PCR)-based methods.
This document has been established for food testing, but is also applicable to other domains using thermal cyclers (e.g. environmental, human health, animal health, forensic testing).
|
Published |
2021-11 |
Edition : 1 |
Number of pages : 27 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/TS 20836:2005 |
Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — Performance testing for thermal cyclers |
ISO/TS 20836:2005 provides basic requirements for the installation, performance and maintenance of thermal cyclers. Although thermal cyclers are robust technical equipment, they do require regular maintenance. Their cooling/heating elements have a limited lifetime. Proper functioning of the cooling/heating element depends both on the quality of the cooling/heating devices and proper use and care.
In addition to outlining the requirement for a defined maintenance programme, procedures are described for the determination of thermal cycler performance by biochemical or physical methods.
|
Withdrawn |
2005-08 |
Edition : 1 |
Number of pages : 13 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 20837:2006 |
Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — Requirements for sample preparation for qualitative detection |
ISO 20837:2006 provides criteria and examples for sample preparation in order to obtain PCR-compatible samples or nucleic acids of suitable quality and quantity for PCR.
It provides a description of the general principles involved. References to standards concerning the enrichment of microorganisms are given in Annex A, and a detailed method for DNA extraction is given in Annex B.
ISO 20837:2006 has been established for food matrices, but could also be applied to feed and agricultural/environmental matrices with some adaptations, if necessary.
|
Published |
2006-04 |
Edition : 1 |
Number of pages : 7 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 20838:2006 |
Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — Requirements for amplification and detection for qualitative methods |
ISO 20838:2006 provides the overall framework for qualitative methods for the detection of food-borne pathogens using the polymerase chain reaction (PCR).
It covers the general requirements for the specific amplification of target nucleic acid sequences and the detection and confirmation of the identity of the amplified nucleic acid sequence.
Guidelines, minimum requirements and performance characteristics described in ISO 20838:2006 are intended to ensure that comparable and reproducible results are obtained in different laboratories.
ISO 20838:2006 has been established for food-borne pathogens in or isolated from food and feed matrices, but can also be applied to other matrices, for example environmental samples, or to the detection of other microorganisms under investigation.
|
Published |
2006-04 |
Edition : 1 |
Number of pages : 7 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 20976-1:2019 |
Microbiology of the food chain — Requirements and guidelines for conducting challenge tests of food and feed products — Part 1: Challenge tests to study growth potential, lag time and maximum growth rate |
This document specifies protocols for conducting microbiological challenge tests for growth studies on vegetative and spore-forming bacteria in raw materials and intermediate or end products.
The use of this document can be extended to yeasts that do not form mycelium.
|
Published |
2019-03 |
Edition : 1 |
Number of pages : 28 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 20976-2:2022 |
Microbiology of the food chain — Requirements and guidelines for conducting challenge tests of food and feed products — Part 2: Challenge tests to study inactivation potential and kinetic parameters |
This document specifies the protocols for conducting microbiological challenge tests for inactivation studies on vegetative bacteria and bacterial spores in the raw materials and ingredients, intermediate or end products.
The use of this document can be extended to yeasts which do not form mycelium.
|
Published |
2022-10 |
Edition : 1 |
Number of pages : 24 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 21187:2004 | IDF 196:2004 |
Milk — Quantitative determination of bacteriological quality — Guidance for establishing and verifying a conversion relationship between routine method results and anchor method results |
ISO 21187|IDF 196:2004 gives guidelines for the establishment of a conversion relationship between the results of a routine method and an anchor method, and its verification for the quantitative determination of the bacteriological quality of milk.
|
Withdrawn |
2004-12 |
Edition : 1 |
Number of pages : 13 |
Technical Committee |
07.100.30
Food microbiology
;
67.100.01
Milk and milk products in general
|
| ISO/FDIS 24382 |
Bee pollen — Specifications |
|
Under development |
|
Edition : 1 |
|
Technical Committee |
65.140
Beekeeping
|
| ISO 4874:1981 |
Tobacco — Sampling of batches of raw material — General principles |
|
Withdrawn |
1981-08 |
Edition : 1 |
Number of pages : 4 |
Technical Committee |
65.160
Tobacco, tobacco products and related equipment
|
| ISO 21187:2021 | IDF 196:2021 |
Milk — Quantitative determination of microbiological quality — Guidance for establishing and verifying a conversion relationship between results of an alternative method and anchor method results |
This document gives guidelines for the establishment of a conversion relationship between the results of an alternative method and an anchor method, and its verification for the quantitative determination of the microbiological quality of milk.
NOTE The conversion relationship can be used a) to convert results from an alternative method to the anchor basis or b) to convert results/limits, expressed on an anchor basis, to results in units of an alternative method.
|
Published |
2021-02 |
Edition : 2 |
Number of pages : 19 |
Technical Committee |
07.100.30
Food microbiology
;
67.100.01
Milk and milk products in general
|
| ISO 21527-1:2008 |
Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of yeasts and moulds — Part 1: Colony count technique in products with water activity greater than 0,95 |
ISO 21527-1:2008 specifies a horizontal method for the enumeration of viable yeasts and moulds in products, intended for human consumption or feeding of animals, having a water activity greater than 0,95 [eggs, meat, dairy products (except milk powder), fruits, vegetables, fresh pastes, etc.], by means of the colony count technique at 25 °C plus or minus 1 °C.
ISO 21527-1:2008 does not allow the enumeration of mould spores. Neither the identification of fungal flora nor the examination of foods for mycotoxins lie within the scope of ISO 21527-1:2008. The method specified in ISO 21527-1:2008 is not suitable for enumeration of heat-resistant fungi, such as Byssochlamys fulva or Byssochlamys nivea, in canned or bottled fruit and vegetables.
|
Published |
2008-07 |
Edition : 1 |
Number of pages : 8 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 21527-2:2008 |
Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of yeasts and moulds — Part 2: Colony count technique in products with water activity less than or equal to 0,95 |
ISO 21527-2:2008 specifies a horizontal method for the enumeration of viable osmophilic yeasts and xerophilic moulds in products intended for human consumption or feeding of animals, having a water activity less than or equal to 0,95 (dry fruits, cakes, jams, dried meat, salted fish, grains, cereals and cereals products, flours, nuts, spices and condiments, etc.), by means of the colony count technique at (25 ± 1) °C.
ISO 21527-2:2008 does not apply to dehydrated products with water activity less than or equal to 0,60 (dehydrated cereals, oleaginous, spices, leguminous plants, seeds, powders for instant drinks, dry products for domestic animals, etc.) and does not allow the enumeration of mould spores. Neither the identification of fungal flora nor the examination of foods for mycotoxins lie within the scope of ISO 21527-2:2008. The method specified in ISO 21527-2:2008 is not suitable for enumeration of halophilic xerophilic fungi (i.e. Polypaecilum pisce, Basipetospora halophila) found in dried fish.
|
Published |
2008-07 |
Edition : 1 |
Number of pages : 9 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/AWI 21527 |
Microbiology of the food chain — Horizontal method for the enumeration of yeasts and moulds |
This document specifies a horizontal method for the enumeration of viable yeasts and moulds. It is applicable to
— products intended for human consumption,
— products intended for animal feeding,
— environmental samples including air samples, in the area of food and feed production, handling and
— samples from the primary production stage.
This horizontal method was originally developed for the examination of all samples belonging to the food chain. Because of the large variety of products in the food chain, it is possible that this horizontal method is not appropriate in every detail for all products. Nevertheless, it is expected that the required modifications are minimized so that they do not result in a significant deviation from this horizontal method.
A surface disinfection procedure is applied to inactivate the surface mycobiota and to study the fungi that invaded the food, often applied in solid foods such as nuts or grains. The method specified in the document does not include analysis of fungi after a surface disinfected step (e.g. using a hypochlorite solution).
Neither the identification of the mycobiota nor the examination of foods for mycotoxins lie within the scope of this document.
The method specified in this document does not include a heat-treatment and is therefore not suitable for detection and/or enumeration of heat-resistant fungi, such as Paecilomyces fulvus (syn. Byssochlamys fulva) or Aspergillus fischeri (syn. Neosartorya fischeri), ) in non-pasteurized samples.
|
Under development |
|
Edition : 1 |
|
Technical Committee |
07.100.30
Food microbiology
|
| ISO 21528-1:2017 |
Microbiology of the food chain — Horizontal method for the detection and enumeration of Enterobacteriaceae — Part 1: Detection of Enterobacteriaceae |
ISO 21528-1:2017 specifies a method, with enrichment, for the detection of Enterobacteriaceae. It is applicable to
- products intended for human consumption and the feeding of animals, and
- environmental samples in the area of primary production, food production and food handling.
This method is applicable
- when the microorganisms sought are expected to need resuscitation by enrichment, and
- when the number sought is expected to be below 100 per millilitre or per gram of test sample.
A limitation on the applicability of ISO 21528-1:2017 is imposed by the susceptibility of the method to a large degree of variability (see Clause 11).
NOTE Enumeration can be carried out by calculation of the most probable number (MPN) after incubation in liquid medium. See Annex A.
|
Published |
2017-06 |
Edition : 2 |
Number of pages : 17 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/CD 24607 |
Honey — Specifications |
This standard applies to all honeys produced by honey bees (Apis ssp.) and covers all styles of honey presentations which are processed and ultimately intended for direct consumption. The honey which is packed for sale in bulk containers, which may be repacked into retail packs is included.
This standard covers honey for industrial uses.
|
Under development |
|
Edition : 1 |
|
Technical Committee |
67.180.10
Sugar and sugar products
|
| ISO 21528-2:2004 |
Microbiology of food and animal feeding stuffs — Horizontal methods for the detection and enumeration of Enterobacteriaceae — Part 2: Colony-count method |
ISO 21528-2:2004 specifies a method, without pre-enrichment, for the enumeration of Enterobacteriaceae. It is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling.
Enumeration is carried out by counting colonies in a solid medium after incubation at 37 °C.
This technique is recommended when the number of colonies sought is expected to be more than 100 per millilitre or per gram of the test sample.
|
Withdrawn |
2004-08 |
Edition : 1 |
Number of pages : 10 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 21528-2:2017 |
Microbiology of the food chain — Horizontal method for the detection and enumeration of Enterobacteriaceae — Part 2: Colony-count technique |
ISO 21528-2:2017 specifies a method for the enumeration of Enterobacteriaceae. It is applicable to
- products intended for human consumption and the feeding of animals, and
- environmental samples in the area of primary production, food production and food handling.
This technique is intended to be used when the number of colonies sought is expected to be more than 100 per millilitre or per gram of the test sample.
The most probable number (MPN) technique, as included in ISO 21528‑1, is generally used when the number sought is expected to be below 100 per millilitre or per gram of test sample.
|
Published |
2017-06 |
Edition : 2 |
Number of pages : 15 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 21567:2004 |
Microbiology of food and animal feeding stuffs — Horizontal method for the detection of Shigella spp. |
ISO 21567:2004 specifies a horizontal method for the detection of Shigella species.
Subject to certain limitations, ISO 21567:2004 is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling.
|
Published |
2004-11 |
Edition : 1 |
Number of pages : 26 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/CD 21722 |
Microbiology of the food chain — Horizontal method for enumeration of enterococci |
This standard specifies a horizontal method for the enumeration of enterococci as faecal indicators in food, feed and environmental samples by the colony-count technique on a selective solid medium (Slanetz and Bartley agar) by an identification scheme based on tetrazolium reduction and formazan production following by confirmation based on split aesculin at 44°C on Bile Aesculin azide Agar.
|
Under development |
|
Edition : 1 |
|
Technical Committee |
07.100.30
Food microbiology
|
| ISO 21807:2004 |
Microbiology of food and animal feeding stuffs — Determination of water activity |
ISO 21807:2004 gives basic principles and requirements for physical methods of determining the water activity of products intended for human consumption and the feeding of animals.
Water activity can be used to predict microbial growth and determine the microbiological stability of a food product, and it also provides an important, quantitatively determinable criterion for estimating the times for which a foodstuff can be kept.
|
Withdrawn |
2004-09 |
Edition : 1 |
Number of pages : 7 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 21871:2006 |
Microbiology of food and animal feeding stuffs — Horizontal method for the determination of low numbers of presumptive Bacillus cereus — Most probable number technique and detection method |
ISO 21871:2006 specifies a horizontal method for the detection or the enumeration of low numbers of viable presumptive Bacillus cereus by means of the most probable number technique. ISO 21871:2006 is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling.
|
Published |
2006-01 |
Edition : 1 |
Number of pages : 14 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 21872-1:2017 |
Microbiology of the food chain — Horizontal method for the determination of Vibrio spp. — Part 1: Detection of potentially enteropathogenic Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus |
ISO 21872-1:2017 specifies a horizontal method for the detection of enteropathogenic Vibrio spp., which causes human illness in or via the intestinal tract. The species detectable by the methods specified include Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus.
ISO 21872-1:2017 is applicable to the following:
- products intended for human consumption and the feeding of animals;
- environmental samples in the area of food production and food handling.
|
Published |
2017-06 |
Edition : 1 |
Number of pages : 33 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/TS 21872-1:2007 |
Microbiology of food and animal feeding stuffs — Horizontal method for the detection of potentially enteropathogenic Vibrio spp. — Part 1: Detection of Vibrio parahaemolyticus and Vibrio cholerae |
ISO/TS 21872-1:2007 specifies a horizontal method for the detection of the two main pathogenic Vibrio species causing intestinal illness in humans: V. parahaemolyticus and V. cholerae.
It is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling.
|
Withdrawn |
2007-04 |
Edition : 1 |
Number of pages : 19 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 21872-1:2017/Amd 1:2023 |
Microbiology of the food chain — Horizontal method for the determination of Vibrio spp. — Part 1: Detection of potentially enteropathogenic Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus — Amendment 1: Inclusion of performance testing of culture media and reagents |
|
Published |
2023-02 |
Edition : 1 |
Number of pages : 5 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/TS 21872-2:2007 |
Microbiology of food and animal feeding stuffs — Horizontal method for the detection of potentially enteropathogenic Vibrio spp. — Part 2: Detection of species other than Vibrio parahaemolyticus and Vibrio cholerae |
ISO/TS 21872-2:2007 specifies a horizontal method for detection of the enteropathogenic Vibrio species, causing illness in or via the intestinal tract, other than Vibrio parahaemolyticus and Vibrio cholerae. The species detectable by the methods specified include Vibrio fluvialis, Vibrio mimicus and Vibrio vulnificus. It is not suitable for the isolation of Vibrio hollisae. Strains of V. parahaemolyticus and V. cholerae may also be detected during the application of this method.
ISO/TS 21872-2:2007 is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling.
This method is not appropriate for the detection of Vibrio metschnikovii as this is oxidase negative.
|
Withdrawn |
2007-04 |
Edition : 1 |
Number of pages : 24 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/TS 21872-2:2020 |
Microbiology of the food chain — Horizontal method for the determination of Vibrio spp. — Part 2: Enumeration of total and potentially enteropathogenic Vibrio parahaemolyticus in seafood using nucleic acid hybridization |
This document specifies a method for the direct enumeration of potentially enteropathogenic V. parahaemolyticus (tdh and/or trh positive) and/or the enumeration of total V. parahaemolyticus in seafood.
|
Published |
2020-10 |
Edition : 1 |
Number of pages : 27 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 22117:2019 |
Microbiology of the food chain — Specific requirements and guidance for proficiency testing by interlaboratory comparison |
This document specifies requirements and gives guidelines for the organization of proficiency testing (PT) schemes for microbiological examinations of
a) foods and beverages,
b) feeding animals,
c) environmental samples from food and feed production and handling, and
d) primary production stages.
This document is also applicable to the microbiological examination of water where water is either used in food production or is regarded as a food in national legislation.
This document relates to the technical organization and implementation of PT schemes, as well as the statistical treatment of results of microbiological examinations.
This document is designed for use with ISO/IEC 17043 and ISO 13528, and deals only with areas where specific or additional details are necessary for PT schemes dealing with microbiological examinations for the areas specified in the first paragraph.
|
Published |
2019-02 |
Edition : 1 |
Number of pages : 33 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/TS 22117:2010 |
Microbiology of food and animal feeding stuffs — Specific requirements and guidance for proficiency testing by interlaboratory comparison |
ISO/TS 22117:2010 gives requirements and guidance for the organization of proficiency testing schemes for microbiological examinations of: a) food and beverages; b) animal feeding stuffs; c) food production environments and food handling; d) primary production stages.
ISO/TS 22117:2010 is also potentially applicable to the microbiological examination of water where water is either used in food production or is regarded as a food in national legislation.
ISO/TS 22117:2010 relates to the technical organization and the implementation of proficiency testing schemes, as well as the statistical treatment of the results of microbiological examinations.
ISO/TS 22117:2010 is designed for use with ISO/IEC 17043 and ISO 13528, and deals only with areas where specific or additional details are necessary for proficiency testing schemes dealing with microbiological analyses for the areas specified in the first paragraph.
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Withdrawn |
2010-11 |
Edition : 1 |
Number of pages : 28 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 22118:2011 |
Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection and quantification of food-borne pathogens — Performance characteristics |
ISO 22118:2010 specifies minimum requirements of performance characteristics for the detection of nucleic acid sequences (DNA or RNA) by molecular methods. ISO 22118:2010 applies to the detection of food-borne pathogens in foodstuffs and isolates obtained from them using molecular detection methods based on the polymerase chain reaction (PCR).
ISO 22118:2010 is also applicable, for example, to the detection of food-borne pathogens in environmental samples and in animal feeding stuffs.
|
Published |
2011-07 |
Edition : 1 |
Number of pages : 10 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 22119:2011 |
Microbiology of food and animal feeding stuffs — Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens — General requirements and definitions |
ISO 22119:2010 defines terms for the detection of food-borne pathogens in foodstuffs, and isolates obtained from them, using the polymerase chain reaction (PCR). ISO 22119:2010 specifies requirements for the amplification and detection of nucleic acid sequences (DNA or RNA after reverse transcription) by real-time PCR.
The minimum requirements laid down in ISO 22119:2010 provide the basis for comparable and reproducible results within individual and between different laboratories.
ISO 22119:2010 is also applicable, for example, to the detection of food-borne pathogens in environmental samples and in animal feeding stuffs.
|
Published |
2011-07 |
Edition : 1 |
Number of pages : 12 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 12824:2016 |
Royal jelly — Specifications |
ISO 12824:2016 specifies the production and sanitary requirements for royal jelly and establishes a series of organoleptic and chemical test methods to control royal jelly quality. It also specifies the requirements of transport, storage, packaging and marking for royal jelly. This International Standard applies to the royal jelly production (collecting, preliminary processing and packaging) and trade links. This International Standard is not applicable to royal jelly products in which other foods are mixed.
|
Published |
2016-09 |
Edition : 1 |
Number of pages : 35 |
Technical Committee |
67.180.10
Sugar and sugar products
|
| ISO 22174:2005 |
Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — General requirements and definitions |
ISO 22174:2004 gives the general requirements for the in vitro amplification of nucleic acid sequences (DNA or RNA). It is applicable to the testing of foodstuffs and isolates obtained from foodstuffs for food-borne pathogens using the polymerase chain reaction (PCR)
The minimum requirements laid down in ISO 22174:2004 are intended to ensure that comparable and reproducible results are obtained in different laboratories.
ISO 22174:2004 has been established for food-borne pathogens in or isolated from food and feed matrices, but is also applicable to other matrices (e.g. environmental samples) and for the detection of non-pathogenic microorganisms.
|
Published |
2005-02 |
Edition : 1 |
Number of pages : 11 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/DIS 22174 |
Microbiology of the food chain — Polymerase chain reaction (PCR) for the detection and quantification of microorganisms — General requirements and definitions |
|
Under development |
|
Edition : 2 |
Number of pages : 21 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 22964:2017 |
Microbiology of the food chain — Horizontal method for the detection of Cronobacter spp. |
ISO 22964:2017 specifies a horizontal method for the detection of Cronobacter spp.
Subject to the limitations discussed in the introduction, this document is applicable to
- food products and ingredients intended for human consumption and the feeding of animals, and
- environmental samples in the area of food production and food handling.
|
Published |
2017-04 |
Edition : 1 |
Number of pages : 23 |
Technical Committee |
07.100.30
Food microbiology
;
67.100.01
Milk and milk products in general
|
| ISO/TS 22964:2006 | IDF/RM 210: 2006 |
Milk and milk products — Detection of Enterobacter sakazakii |
ISO/TS 22964|IDF/RM 210:2006 specifies a method for the detection of Enterobacter sakazakii in milk powder and powdered infant formula.
The method is also applicable to environmental samples collected from milk powder or infant formula factories.
|
Withdrawn |
2006-02 |
Edition : 1 |
Number of pages : 13 |
Technical Committee |
07.100.30
Food microbiology
;
67.100.01
Milk and milk products in general
|
| ISO 23036-1:2021 |
Microbiology of the food chain — Methods for the detection of Anisakidae L3 larvae in fish and fishery products — Part 1: UV-press method |
This document specifies a method for the detection of Anisakidae L3 larvae commonly found in marine and anadromous fishes. The method is applicable to fresh fish and/or frozen fish, as well as lightly processed fish products, such as marinated, salted or cold smoked.
This method is applicable to quantifying parasitic infections by estimating the number of parasites in the fish musculature.
This method does not apply to determining the species or genotype of detected parasites. Final identification is made by morphological and/or molecular methods.
|
Published |
2021-04 |
Edition : 1 |
Number of pages : 9 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 23036-2:2021 |
Microbiology of the food chain — Methods for the detection of Anisakidae L3 larvae in fish and fishery products — Part 2: Artificial digestion method |
This document specifies a method for the detection of Anisakidae L3 larvae commonly found in marine and anadromous fishes. The method is applicable to fresh fish and/or frozen fish, as well as lightly processed fish products, such as marinated, salted or smoked. It is also suitable for visceral organs as a confirmatory method for a visual inspection scheme.
The artificial digestion method[4][5][6] is applicable to quantifying parasitic infections by estimating the number of parasites in the fish musculature and, when applied to fresh fish or lightly processed fish products (never frozen before processing), determining the viability of Anisakidae L3, which can be present.
This method does not apply to determining the species or genotype of detected parasites. Final identification is made by morphological and/or molecular methods.
|
Published |
2021-04 |
Edition : 1 |
Number of pages : 9 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 23418:2022 |
Microbiology of the food chain — Whole genome sequencing for typing and genomic characterization of bacteria — General requirements and guidance |
This document specifies the minimum requirements for generating and analysing whole genome sequencing (WGS) data of bacteria obtained from the food chain. This process can include the following stages:
a) handling of bacterial cultures;
b) axenic genomic DNA isolation;
c) library preparation, sequencing, and assessment of raw DNA sequence read quality and storage;
d) bioinformatics analysis for determining genetic relatedness, genetic content and predicting phenotype, and bioinformatics pipeline validation;
e) metadata capture and sequence repository deposition;
f) validation of the end-to-end WGS workflow (fit for purpose for intended application).
This document is applicable to bacteria isolated from:
— products intended for human consumption;
— products intended for animal feed;
— environmental samples from food and feed handling and production areas;
— samples from the primary production stage.
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Published |
2022-06 |
Edition : 1 |
Number of pages : 45 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/FDIS 24364 |
Royal jelly production |
|
Under development |
|
Edition : 1 |
|
Technical Committee |
65.140
Beekeeping
|
| ISO/DIS 24381 |
Bee propolis — Specifications |
|
Under development |
|
Edition : 1 |
Number of pages : 40 |
Technical Committee |
65.140
Beekeeping
|
| ISO 10990-4:1999 |
Animal (mammal) traps — Part 4: Methods for testing killing-trap systems used on land or underwater |
This part of ISO 10990 specifies methods for use in performance testing of:
traps used on land to kill mammals;
traps used in submersion sets to kill semi-aquatic mammals.
The performance testing includes methods for evaluation of effective killing, pathological evaluation, mechanical
properties (see note), selectivity, capture efficiency and user safety.
NOTE ISO/TC 191 recognizes that other trapping systems are under development but are at a preliminary stage.
Consequently, inclusion of appropriate mechanical tests for such traps is premature at this time. Nevertheless, the committee
recommends and supports the development of new types of traps and trapping systems together with appropriate testing
methodologies. ISO/TC 191 further recommends that the issue receives in-depth (re)consideration at the five-year review and
that all appropriate advances in technology are incorporated through the deliberations of the committee at that time.
|
Published |
1999-11 |
Edition : 1 |
Number of pages : 23 |
Technical Committee |
65.145
Hunting
|
| ISO 10990-5:1999 |
Animal (mammal) traps — Part 5: Methods for testing restraining traps |
1.1 This part of ISO 10990 specifies methods for use in performance testing of traps used on land to restrain
mammals. The performance testing includes methods for evaluation of trauma, selectivity, capture efficiency and
user safety.
1.2 It is recognized that injury is only one component of animal welfare. However, there are insufficient data
collected in a scientific manner on the additional components to allow for the complete assessment of animal
welfare. Several areas of investigation are presented for evaluation in annex A. Selection of the data collection
methodology is left to the investigator. However, it is assumed that such collection methods will follow accepted
practices.
It should also be understood that data collected in any, or all, of the suggested areas will probably not provide an
absolute measure of welfare. Rather, the compilation of such data over time should provide a mechanism for
comparing the relative animal welfare impacts of different restraint methods.
|
Published |
1999-11 |
Edition : 1 |
Number of pages : 20 |
Technical Committee |
65.145
Hunting
|
| ISO 858:1973 |
Fishing nets — Designation of netting yarns in the Tex System |
As a general rule, netting yarns designated by their linear density or their resultant linear density are usually grey yarns without any preparation. Specifies general usage and complete designation and, for particular cases, brief designation. Three examples for designation are covered. - Replaces ISO Recommendation R 858-1968.
|
Published |
1973-12 |
Edition : 1 |
Number of pages : 2 |
Technical Committee |
65.150
Fishing and fish breeding
|
| ISO 1107:1974 |
Fishing nets — Netting — Basic terms and definitions |
Provides examples (with figure) for the determination of length of mesh side, length of mesh, opening of mesh, direction in knotted netting, direction in knotless netting, and size of netting. - Replaces ISO Recommendation R 1107-1969.
|
Withdrawn |
1974-02 |
Edition : 1 |
Number of pages : 3 |
Technical Committee |
65.150
Fishing and fish breeding
;
01.040.65
Agriculture (Vocabularies)
|
| ISO 1107:2003 |
Fishing nets — Netting — Basic terms and definitions |
ISO 1107:2003 gives the principal terms relating to netting for fishing nets, together with their definitions or, in some cases, the method of expressing dimensions.
|
Withdrawn |
2003-06 |
Edition : 2 |
Number of pages : 7 |
Technical Committee |
65.150
Fishing and fish breeding
;
01.040.65
Agriculture (Vocabularies)
|
| ISO 1107:2017 |
Fishing nets — Netting — Basic terms and definitions |
ISO 1107:2017 gives the principal terms relating to netting for fishing nets, together with their definitions or, in some cases, the method of expressing dimensions.
|
Published |
2017-08 |
Edition : 3 |
Number of pages : 8 |
Technical Committee |
65.150
Fishing and fish breeding
;
01.040.65
Agriculture (Vocabularies)
|