| Name |
Description |
Abstract |
Status |
Publication date |
Edition |
Number of pages |
Technical committee |
ICS |
| ISO 6888-2:1999/Amd 1:2003 |
Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) — Part 2: Technique using rabbit plasma fibrinogen agar medium — Amendment 1: Inclusion of precision data |
|
Withdrawn |
2003-07 |
Edition : 1 |
Number of pages : 6 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 6887-5:2010 |
Microbiology of food and animal feeding stuffs — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 5: Specific rules for the preparation of milk and milk products |
ISO 6887-5:2010 specifies rules for the preparation of samples of milk and milk products and their suspension for microbiological examination when the samples require a different preparation from the general methods specified in ISO 6887-1. ISO 6887-1 defines the general rules for the preparation of the initial suspension and decimal dilutions for microbiological examination.
ISO 6887-5:2010 excludes preparation of samples for both enumeration and detection test methods where preparation details are specified in the relevant International Standards.
ISO 6887-5:2010 is applicable to: milk and liquid milk products; dried milk products; cheese; casein and caseinates; butter; ice-cream; custard, desserts and sweet cream; fermented milk and sour cream; and milk-based infant foods.
|
Withdrawn |
2010-08 |
Edition : 1 |
Number of pages : 13 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 6887-5:2020 |
Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 5: Specific rules for the preparation of milk and milk products |
This document specifies rules for the preparation of samples of milk and milk products and their suspensions for microbiological examination when the samples require a different preparation from the general methods specified in ISO 6887-1.
This document excludes the preparation of samples for both enumeration and detection test methods where preparation details are specified in the relevant International Standards.
This document is intended to be used in conjunction with ISO 6887-1.
This document is applicable to:
a) milk and liquid milk products;
b) dehydrated milk products;
c) cheese and cheese products;
d) casein and caseinates;
e) butter;
f) milk-based ice-cream;
g) milk-based custard, desserts and sweet cream;
h) fermented milks, yogurt, probiotics milk products and sour cream;
i) dehydrated milk-based infant foods, with or without probiotics.
|
Published |
2020-04 |
Edition : 2 |
Number of pages : 11 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 6887-6:2013 |
Microbiology of food and animal feed — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 6: Specific rules for the preparation of samples taken at the primary production stage |
ISO 6887-6:2013 specifies rules for the preparation of samples taken at all stages from the farm to the slaughterhouse and their suspension for microbiological examination when the samples require different preparation from the methods described in ISO 6887-1.
ISO 6887-6:2013 excludes the preparation of samples for both enumeration and detection test methods where preparation details are specified in the relevant International Standards.
ISO 6887-6:2013 is applicable to various samples taken from the hatchery, the farm, from the vehicle or the animals during transportation, or from animals or their carcasses in the slaughterhouse, to indicate the microbiological status of the animals in relation to zoonotic agents. ISO 6887-6:2013 does not apply to samples taken to assess the hygiene of meat.
|
Published |
2013-03 |
Edition : 1 |
Number of pages : 10 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 6888-1:1999 |
Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) — Part 1: Technique using Baird-Parker agar medium |
|
Withdrawn |
1999-02 |
Edition : 1 |
Number of pages : 11 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 6888-1:1999/Amd 1:2003 |
Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) — Part 1: Technique using Baird-Parker agar medium — Amendment 1: Inclusion of precision data |
|
Withdrawn |
2003-07 |
Edition : 1 |
Number of pages : 6 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 6888-1:1999/Amd 2:2018 |
Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) — Part 1: Technique using Baird-Parker agar medium — Amendment 2: Inclusion of an alternative confirmation test using RPFA stab method |
|
Withdrawn |
2018-07 |
Edition : 1 |
Number of pages : 5 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 6888-1:2021 |
Microbiology of the food chain — Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) — Part 1: Method using Baird-Parker agar medium |
This document specifies a horizontal method for the enumeration of coagulase-positive staphylococci by counting the colonies obtained on a solid medium (Baird-Parker medium)[10] after aerobic incubation at 34 °C to 38 °C and coagulase confirmation.
This document is applicable to:
— products intended for human consumption;
— products intended for animal feeding;
— environmental samples in the area of food and feed production, handling, and
— samples from the primary production stage.
This horizontal method was originally developed for the examination of all samples belonging to the food chain.
Because of the large variety of products in the food chain, it is possible that this horizontal method is not appropriate in every detail for all products. Nevertheless, it is expected that the required modifications are minimized so that they do not result in a significant deviation from this horizontal method.
Based on the information available at the time of publication of this document, this method is not considered to be (fully) suited to the examination of fermented products or other products containing technological flora based on Staphylococcus spp (e.g. S. xylosus) (such as cheeses made from raw milk and certain raw meat products) likely to be contaminated by:
— staphylococci forming atypical colonies on a Baird-Parker agar medium;
— background flora that can obscure the colonies being sought.
Nevertheless, both this document and ISO 6888-2 are given equivalent status.
|
Published |
2021-08 |
Edition : 2 |
Number of pages : 20 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 6888-1:2021/DAmd 1 |
Microbiology of the food chain — Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) — Part 1: Method using Baird-Parker agar medium — Amendment 1: Corrections |
|
Under development |
|
Edition : 2 |
Number of pages : 2 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 6888-2:2021 |
Microbiology of the food chain — Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) — Part 2: Method using rabbit plasma fibrinogen agar medium |
This document specifies a horizontal method for the enumeration of coagulase-positive staphylococci by counting the colonies obtained on a solid medium (rabbit plasma fibrinogen agar medium) after aerobic incubation at 34 °C to 38 °C (see Reference [10]).
This document is applicable to:
— products intended for human consumption;
— products intended for animal feeding;
— environmental samples in the area of food and feed production and handling;
— samples from the primary production stage.
This horizontal method was originally developed for the examination of all samples belonging to the food chain.
Because of the large variety of products in the food chain, it is possible that this horizontal method is not appropriate in every detail for all products. Nevertheless, it is expected that the required modifications are minimized so that they do not result in a significant deviation from this horizontal method.
Based on the information available at the time of publication of this document, this method is not considered to be (fully) suited to the examination of fermented products or other products containing technological flora based on Staphylococcus spp. (e.g. S. xylosus) (such as cheeses made from raw milk and certain raw meat products) likely to be contaminated by:
— staphylococci forming atypical colonies on a Baird-Parker agar medium;
— background flora that can obscure the colonies being sought.
Nevertheless, both ISO 6888-1 and this document are given equivalent status.
|
Published |
2021-08 |
Edition : 2 |
Number of pages : 14 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 6888-2:2021/DAmd 1 |
Microbiology of the food chain — Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) — Part 2: Method using rabbit plasma fibrinogen agar medium — Amendment 1: Corrections |
|
Under development |
|
Edition : 2 |
Number of pages : 1 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 6888-3:2003 |
Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) — Part 3: Detection and MPN technique for low numbers |
ISO 6888-3:2003 specifies a horizontal method for the enumeration and detection of coagulase-positive staphylococci, using the most probable number (MPN) technique. It is applicable to
products intended for human consumption and the feeding of animals, andenvironmental samples in the area of food production and food handling.
This method is recommended for products where staphylococci are expected to be stressed and in low numbers as, for example, in dried products. Coagulase-positive staphylococci will primarily be Staphylococcus aureus but Staphylococcus intermedius and some strains of Staphylococcus hyicus also produce coagulase.
|
Published |
2003-03 |
Edition : 1 |
Number of pages : 11 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/CD 6888-3 |
Microbiology of the food chain — Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) — Part 3: Detection and MPN technique for low numbers |
|
Deleted |
|
Edition : 2 |
|
Technical Committee |
07.100.30
Food microbiology
|
| ISO 7218:1996 |
Microbiology of food and animal feeding stuffs — General rules for microbiological examinations |
Gives general instructions for carrying out microbiological examinations in accordance with specific standards. The purpose is to help to ensure the validity of the examinations, to ascertain that the general techniques used for conducting these examinations are the same in all laboratories. May be used wholly or partly for the accreditation of a test laboratory by national organizations.
|
Withdrawn |
1996-02 |
Edition : 2 |
Number of pages : 43 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 7218:1996/Amd 1:2001 |
Microbiology of food and animal feeding stuffs — General rules for microbiological examinations — Amendment 1 |
|
Withdrawn |
2001-04 |
Edition : 2 |
Number of pages : 7 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 7218:2007 |
Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations |
ISO 7218:2007 gives general requirements and guidance/options intended for three main uses:
implementation of ISO TC34/SC9 or TC34/SC5 standards for detection or enumeration of microorganisms;good laboratory practices for food microbiological laboratories (the purpose is not to detail them in this International Standard, manuals are available for that purpose);guidance for accreditation of food microbiological laboratories (this International Standard describes the technical requirements according to Appendix B of ISO/IEC 17025:2005 for the accreditation of a microbiological laboratory by national organizations).
ISO 7218:2007 covers examination for bacteria, yeasts and moulds and can be used if supplemented with specific guidance for prions, parasites and viruses. It does not cover the examination for toxins or other metabolites (e.g. amines) from microorganisms.
It applies to the microbiology of food, animal feeding stuffs, the food production environment and the primary production environment.
The purpose of ISO 7218:2007 is to help to ensure the validity of food microbiology examinations, to assist in ensuring that the general techniques used for conducting these examinations are the same in all laboratories, to help achieve homogeneous results in different laboratories, and to contribute towards the safety of the laboratory personnel by preventing risks of infection.
|
Published |
2007-08 |
Edition : 3 |
Number of pages : 66 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 7218:2007/Amd 1:2013 |
Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations — Amendment 1 |
|
Published |
2013-08 |
Edition : 3 |
Number of pages : 66 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/DIS 7218 |
Microbiology of the food chain — General requirements and guidance for microbiological examinations |
|
Under development |
|
Edition : 4 |
Number of pages : 88 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 7251:2005 |
Microbiology of food and animal feeding stuffs — Horizontal method for the detection and enumeration of presumptive Escherichia coli — Most probable number technique |
ISO 7251:2005 gives general guidelines for the detection and enumeration of presumptive Escherichia coli by means of the liquid-medium culture technique and calculation of the most probable number (MPN) after incubation at 37 °C, then at 44 °C. This International Standard is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling.
|
Published |
2005-02 |
Edition : 3 |
Number of pages : 13 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 7251:2005/DAmd 1 |
Microbiology of food and animal feeding stuffs — Horizontal method for the detection and enumeration of presumptive Escherichia coli — Most probable number technique — Amendment 1: Inclusion of performance testing of culture media and reagents |
|
Under development |
|
Edition : 3 |
Number of pages : 2 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 7698:1990 |
Cereals, pulses and derived products — Enumeration of bacteria, yeasts and moulds |
|
Withdrawn |
1990-04 |
Edition : 1 |
Number of pages : 6 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 7889:2003 | IDF 117:2003 |
Yogurt — Enumeration of characteristic microorganisms — Colony-count technique at 37 degrees C |
ISO 7889|IDF 117:2003 specifies a method for the enumeration of characteristic microorganisms in yogurt by means of the colony-count technique at 37 degrees Celsius.
The method is applicable to yogurts in which both characteristic microorganisms (Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus) are present and viable.
|
Published |
2003-02 |
Edition : 1 |
Number of pages : 11 |
Technical Committee |
07.100.30
Food microbiology
;
67.100.99
Other milk products
|
| ISO/CD 7889 | IDF 117 |
Yogurt — Enumeration of characteristic microorganisms — Colony-count technique |
|
Under development |
|
Edition : 2 |
|
Technical Committee |
07.100.30
Food microbiology
;
67.100.99
Other milk products
|
| ISO 7932:2004 |
Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of presumptive Bacillus cereus — Colony-count technique at 30 degrees C |
ISO 7932:2004 specifies a horizontal method for the enumeration of viable Bacillus cereus by means of the colony-count technique at 30 °C. It is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling.
In order to have a practicable test method, the confirmatory stage has been restricted to the typical aspect on MYP agar and the haemolysis test. Thus the term presumptive has been introduced in order to acknowledge the fact that the confirmatory stage does not enable the distinction of B. cereus from other closely related but less commonly encountered Bacillus species, such as B. anthracis, B. thuringiensis, B. weihenstephanensis, B. mycoides. An additional motility test may help to differentiate B. cereus from B. anthracis in cases where the presence of the latter is suspected.
|
Published |
2004-06 |
Edition : 3 |
Number of pages : 13 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 7932:2004/Amd 1:2020 |
Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of presumptive Bacillus cereus — Colony-count technique at 30 degrees C — Amendment 1: Inclusion of optional tests |
|
Published |
2020-03 |
Edition : 3 |
Number of pages : 27 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 7937:1997 |
Microbiology of food and animal feeding stuffs — Horizontal method for enumeration of Clostridium perfringens — Colony-count technique |
|
Withdrawn |
1997-04 |
Edition : 2 |
Number of pages : 10 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 7937:2004 |
Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of Clostridium perfringens — Colony-count technique |
ISO 7937:2004 describes a horizontal method for the enumeration of viable Clostridium perfringens. It is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling.
|
Published |
2004-08 |
Edition : 3 |
Number of pages : 16 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 8261:1989 |
Milk and milk products — Preparation of test samples and dilutions for microbiological examination |
|
Withdrawn |
1989-06 |
Edition : 1 |
Number of pages : 5 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 8261:2001 | IDF 122: 2001 |
Milk and milk products — General guidance for the preparation of test samples, initial suspensions and decimal dilutions for microbiological examination |
ISO 8261|IDF 122:2001 describes general guidelines for the preparation of test samples, initial suspensions and decimal dilutions for the microbiological examination of milk and milk products, including milk-based infant foods.
|
Withdrawn |
2001-06 |
Edition : 2 |
Number of pages : 12 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 17410:2001 |
Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of psychrotrophic microorganisms |
|
Withdrawn |
2001-05 |
Edition : 1 |
Number of pages : 7 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 8552:2004 | IDF 132:2004 |
Milk — Estimation of psychrotrophic microorganisms — Colony-count technique at 21 degrees C (Rapid method) |
ISO 8552|IDF 132:2004 specifies a rapid method for estimating the number of psychrotrophic microorganisms by means of the colony-count technique at 21 °C.
The method is applicable to raw and pasteurized milk.
|
Withdrawn |
2004-05 |
Edition : 1 |
Number of pages : 6 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 8553:2004 | IDF 131:2004 |
Milk — Enumeration of microorganisms — Plate-loop technique at 30 degrees C |
ISO 8553|IDF 131:2004 specifies a method for the enumeration of microorganisms in raw milk by using the plate loop technique at 30 °C.
|
Published |
2004-05 |
Edition : 1 |
Number of pages : 7 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 9232:2003 | IDF 146:2003 |
Yogurt — Identification of characteristic microorganisms (Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus) |
ISO 9232|IDF 146:2003 specifies tests for the identification of the characteristic microorganisms in yogurt on the basis of their morphological, cultural and physiological properties.
It is applicable to strains isolated from yogurts in which both characteristic microorganisms are present and viable.
|
Published |
2003-02 |
Edition : 1 |
Number of pages : 17 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 9232:2003/FDAmd 1 | IDF 146:2003/AMD.1 |
Yogurt — Identification of characteristic microorganisms (Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus) — Amendment 1: Inclusion of performance testing of culture media and reagents |
|
Under development |
|
Edition : 1 |
Number of pages : 4 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 10272-1:2006 |
Microbiology of food and animal feeding stuffs — Horizontal method for detection and enumeration of Campylobacter spp. — Part 1: Detection method |
ISO 10272-1:2006 describes a horizontal method for the detection of Campylobacter spp.
It is applicable to products intended for human consumption or for the feeding of animals, and to environmental samples in the area of food production and food handling, subject to the limitations stated in the Introduction.
|
Withdrawn |
2006-01 |
Edition : 1 |
Number of pages : 16 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 10272-1:2017 |
Microbiology of the food chain — Horizontal method for detection and enumeration of Campylobacter spp. — Part 1: Detection method |
ISO 10272-1:2017 specifies a horizontal method for the detection by enrichment or direct plating of Campylobacter spp. It is applicable to
- products intended for human consumption,
- products intended for animal feeding,
- environmental samples in the area of food and feed production, handling, and
- samples from the primary production stage such as animal faeces, dust, and swabs.
|
Published |
2017-06 |
Edition : 2 |
Number of pages : 24 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 10272-1:2017/Amd 1:2023 |
Microbiology of the food chain — Horizontal method for detection and enumeration of Campylobacter spp. — Part 1: Detection method — Amendment 1: Inclusion of methods for molecular confirmation and identification of thermotolerant Campylobacter spp., the use of growth supplement in Preston broth and changes in the performance testing of culture media |
|
Published |
2023-01 |
Edition : 2 |
Number of pages : 26 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 10272-2:2017 |
Microbiology of the food chain — Horizontal method for detection and enumeration of Campylobacter spp. — Part 2: Colony-count technique |
ISO 10272-2:2017 specifies a horizontal method for the enumeration of Campylobacter spp. It is applicable to
- products intended for human consumption,
- products intended for animal feeding,
- environmental samples in the area of food and feed production, handling, and
- samples from the primary production stage such as animal faeces, dust, and swabs.
|
Published |
2017-06 |
Edition : 1 |
Number of pages : 19 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/TS 10272-2:2006 |
Microbiology of food and animal feeding stuffs — Horizontal method for detection and enumeration of Campylobacter spp. — Part 2: Colony-count technique |
ISO/TS 10272-2:2006 describes a horizontal method for the enumeration of Campylobacter spp.
It is applicable to products intended for human consumption or for the feeding of animals, and to environmental samples in the area of food production and food handling, subject to the limitations stated in the Introduction.
|
Withdrawn |
2006-01 |
Edition : 1 |
Number of pages : 13 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 10272-2:2017/Amd 1:2023 |
Microbiology of the food chain — Horizontal method for detection and enumeration of Campylobacter spp. — Part 2: Colony-count technique — Amendment 1: Inclusion of methods for molecular confirmation and identification of thermotolerant Campylobacter spp. and changes in the performance testing of culture media |
|
Published |
2023-01 |
Edition : 1 |
Number of pages : 24 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/DIS 15213-2 |
Microbiology of the food chain — Horizontal method for the detection and enumeration of Clostridium spp. — Part 2: Enumeration of Clostridium perfringens by colony-count technique |
|
Under development |
|
Edition : 1 |
Number of pages : 42 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/TS 10272-3:2010 |
Microbiology of food and animal feeding stuffs — Horizontal method for detection and enumeration of Campylobacter spp. — Part 3: Semi-quantitative method |
ISO/TS 10272-3:2010 describes a horizontal method for the semi-quantitative determination of Campylobacter spp.
It is applicable to products intended for human consumption or for the feeding of animals, and to environmental samples in the area of food production and food handling. However, it is possible that ISO/TS 10272-3:2010 is not appropriate in every detail for certain products, deviations from it being made necessary for technical reasons. It is possible that ISO/TS 10272-3:2010 is not applicable at all to some other products.
|
Withdrawn |
2010-03 |
Edition : 1 |
Number of pages : 17 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/TS 10272-3:2010/Cor 1:2011 |
Microbiology of food and animal feeding stuffs — Horizontal method for detection and enumeration of Campylobacter spp. — Part 3: Semi-quantitative method — Technical Corrigendum 1 |
|
Withdrawn |
2011-12 |
Edition : 1 |
Number of pages : 2 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 10272:1995 |
Microbiology of food and animal feeding stuffs — Horizontal method for detection of thermotolerant Campylobacter |
Describes a horizontal method for the detection of thermotolerant Campylobacter in food and animal feeding stuffs.
|
Withdrawn |
1995-09 |
Edition : 1 |
Number of pages : 15 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 10272:1995/Cor 1:1996 |
Microbiology of food and animal feeding stuffs — Horizontal method for detection of thermotolerant Campylobacter — Technical Corrigendum 1 |
Contains a great number of corrections regarding the subclauses 1, 9.5.5.1, 9.6, B.2.4.1, B.5.3.1 and B.7.3.1.
|
Withdrawn |
1996-06 |
Edition : 1 |
Number of pages : 1 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 10272:1995/Cor 2:1997 |
Microbiology of food and animal feeding stuffs — Horizontal method for detection of thermotolerant Campylobacter — Technical Corrigendum 2 |
|
Withdrawn |
1997-07 |
Edition : 1 |
Number of pages : 1 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 10273:2003 |
Microbiology of food and animal feeding stuffs — Horizontal method for the detection of presumptive pathogenic Yersinia enterocolitica |
ISO 10273:2003 specifies a horizontal method for the detection of Yersinia enterocolitica presumed to be pathogenic to human subjects. It is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling.
|
Withdrawn |
2003-06 |
Edition : 2 |
Number of pages : 31 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 10273:2017 |
Microbiology of the food chain — Horizontal method for the detection of pathogenic Yersinia enterocolitica |
ISO 10273:2017 specifies a horizontal method for the detection of Y. enterocolitica associated with human disease. It is applicable to
- products intended for human consumption and the feeding of animals, and
- environmental samples in the area of food production and food handling.
|
Published |
2017-03 |
Edition : 3 |
Number of pages : 40 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 10560:1993 |
Milk and milk products — Detection of Listeria monocytogenes |
The principle of the method specified comprises (at least) the following three stages: enrichment in selective liquid medium, isolation and presumptive identification, confirmation.
|
Withdrawn |
1993-07 |
Edition : 1 |
Number of pages : 12 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 10560:1993/Cor 1:1994 |
Milk and milk products — Detection of Listeria monocytogenes — Technical Corrigendum 1 |
|
Withdrawn |
1994-04 |
Edition : 1 |
Number of pages : 1 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/TS 11133-1:2000 |
Microbiology of food and animal feeding stuffs — Guidelines on preparation and production of culture media — Part 1: General guidelines on quality assurance for the preparation of culture media in the laboratory |
|
Withdrawn |
2000-06 |
Edition : 1 |
Number of pages : 15 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/TS 11133-1:2009 |
Microbiology of food and animal feeding stuffs — Guidelines on preparation and production of culture media — Part 1: General guidelines on quality assurance for the preparation of culture media in the laboratory |
ISO/TS 11133-1:2009 provides the general terminology related to quality assurance and specifies the minimum requirements for the preparation of culture media to be used for the microbiological analysis of products intended for human consumption or animal feeding.
It is also applicable to culture media to be used for the microbiological analysis of all kinds of water.
These requirements are applicable to four categories of culture media used in laboratories that prepare and/or use culture media for performing microbiological analyses: commercially manufactured ready-to-use media; media to be remelted, supplemented and distributed; media prepared from commercially available dehydrated formulations; and media prepared from their individual components.
|
Withdrawn |
2009-02 |
Edition : 2 |
Number of pages : 18 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/CD TS 15213-3 |
Microbiology of the food chain — Horizontal method for the detection and enumeration of Clostridium spp. — Part 3: Detection of Clostridium perfringens |
|
Under development |
|
Edition : 1 |
|
Technical Committee |
07.100.30
Food microbiology
|
| ISO/TS 11133-2:2003 |
Microbiology of food and animal feeding stuffs — Guidelines on preparation and production of culture media — Part 2: Practical guidelines on performance testing of culture media |
ISO/TS 11133-2:2003 sets criteria and methods for the performance testing of culture media. It applies to:
commercial bodies producing and/or distributing ready-to-use or semi-finished reconstituted or dehydrated media to microbiological laboratories;non-commercial bodies supplying media to third parties;microbiological laboratories preparing culture media for their own use and evaluating the performance of these media.
|
Withdrawn |
2003-12 |
Edition : 1 |
Number of pages : 23 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/TS 11133-2:2003/Amd 1:2011 |
Microbiology of food and animal feeding stuffs — Guidelines on preparation and production of culture media — Part 2: Practical guidelines on performance testing of culture media — Amendment 1: Test microorganisms for commonly used culture media |
|
Withdrawn |
2011-02 |
Edition : 1 |
Number of pages : 12 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 11133:2014 |
Microbiology of food, animal feed and water — Preparation, production, storage and performance testing of culture media |
ISO 11133:2014 defines terms related to quality assurance of culture media and specifies the requirements for the preparation of culture media intended for the microbiological analysis of food, animal feed, and samples from the food or feed production environment as well as all kinds of water intended for consumption or used in food production. These requirements are applicable to all categories of culture media prepared for use in laboratories performing microbiological analyses.
ISO 11133:2014 also sets criteria and describes methods for the performance testing of culture media. It applies to producers such as:
commercial bodies producing and/or distributing ready-to-use or semi-finished reconstituted or dehydrated media;
non-commercial bodies supplying media to third parties;
microbiological laboratories preparing culture media for their own use.
|
Published |
2014-05 |
Edition : 1 |
Number of pages : 99 |
Technical Committee |
07.100.20
Microbiology of water
;
07.100.30
Food microbiology
|
| ISO 11133:2014/Amd 1:2018 |
Microbiology of food, animal feed and water — Preparation, production, storage and performance testing of culture media — Amendment 1 |
|
Published |
2018-02 |
Edition : 1 |
Number of pages : 7 |
Technical Committee |
07.100.20
Microbiology of water
;
07.100.30
Food microbiology
|
| ISO 11133:2014/Amd 2:2020 |
Microbiology of food, animal feed and water — Preparation, production, storage and performance testing of culture media — Amendment 2 |
|
Published |
2020-05 |
Edition : 1 |
Number of pages : 13 |
Technical Committee |
07.100.20
Microbiology of water
;
07.100.30
Food microbiology
|
| ISO/AWI 11133 |
Microbiology of food, animal feed and water — Preparation, production, storage and performance testing of culture media |
|
Under development |
|
Edition : 2 |
|
Technical Committee |
07.100.20
Microbiology of water
;
07.100.30
Food microbiology
|
| ISO 11290-1:1996 |
Microbiology of food and animal feeding stuffs — Horizontal method for the detection and enumeration of Listeria monocytogenes — Part 1: Detection method |
Specifies a horizontal method for the detection of Listeria monocytogenes. Applicable to products intended for human consumption or as animal feeding stuffs.
|
Withdrawn |
1996-12 |
Edition : 1 |
Number of pages : 16 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 11290-1:1996/Amd 1:2004 |
Microbiology of food and animal feeding stuffs — Horizontal method for the detection and enumeration of Listeria monocytogenes — Part 1: Detection method — Amendment 1: Modification of the isolation media and the haemolysis test, and inclusion of precision data |
|
Withdrawn |
2004-10 |
Edition : 1 |
Number of pages : 13 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 11290-1:2017 |
Microbiology of the food chain — Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. — Part 1: Detection method |
ISO 11290-1:2017 specifies a horizontal method for
- the detection of L. monocytogenes, and
- the detection of Listeria spp. (including L. monocytogenes).
ISO 11290-1:2017 is applicable to
- products intended for human consumption and for the feeding of animals, and
- environmental samples in the area of food production and food handling.
|
Published |
2017-05 |
Edition : 2 |
Number of pages : 36 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 11290-2:1998 |
Microbiology of food and animal feeding stuffs — Horizontal method for the detection and enumeration of Listeria monocytogenes — Part 2: Enumeration method |
|
Withdrawn |
1998-06 |
Edition : 1 |
Number of pages : 20 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 11290-2:1998/Amd 1:2004 |
Microbiology of food and animal feeding stuffs — Horizontal method for the detection and enumeration of Listeria monocytogenes — Part 2: Enumeration method — Amendment 1: Modification of the enumeration medium |
|
Withdrawn |
2004-10 |
Edition : 1 |
Number of pages : 4 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 11290-2:2017 |
Microbiology of the food chain — Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. — Part 2: Enumeration method |
ISO 11290-2:2017 specifies a horizontal method for
- the enumeration of L. monocytogenes, and
- the enumeration of Listeria spp. (including L. monocytogenes).
ISO 11290-2:2017 is applicable to
- products intended for human consumption and for the feeding of animals, and
- environmental samples in the area of food production and food handling.
|
Published |
2017-05 |
Edition : 2 |
Number of pages : 29 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 11866-1:1997 |
Milk and milk products — Enumeration of presumptive Escherichia coli — Part 1: Most probable number technique |
Relates to a method for the enumeration of presumptive Escherichia coli by means of the culture technique involving a liquid medium, and calculation of the number of Escherichia coli per gram or per millilitre by the most probable number (MPN) after incubation at 37 °C.
|
Withdrawn |
1997-01 |
Edition : 1 |
Number of pages : 11 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 11866-1:2005 | IDF 170-1:2005 |
Milk and milk products — Enumeration of presumptive Escherichia coli — Part 1: Most probable number technique using 4-methylumbelliferyl-beta-D-glucuronide (MUG) |
ISO 11866-1|IDF 170-1:2005 specifies a combined method for the enumeration of presumptive Escherichia coli and of presumptive coliforms by means of a culture technique involving a liquid medium with MUG, and calculation of the number of presumptive Escherichia coli and/or coliforms per gram or per millilitre by the most probable number (MPN) technique after incubation at 30 °C.
The method is applicable to milk, liquid milk products, dried milk, dried sweet whey, dried buttermilk, lactose, acid casein, lactic casein and rennet casein, caseinate and dried acid whey, cheese and processed cheese, butter, frozen milk products (including edible ices), and custard, desserts and cream.
The method specified in ISO 11866-1|IDF 170-1:2005 is preferred for samples in which comparatively low numbers of presumptive Escherichia coli and/or other presumptive coliforms (less than 100 per gram or 10 per millilitre) are suspected.
|
Published |
2005-12 |
Edition : 2 |
Number of pages : 11 |
Technical Committee |
07.100.30
Food microbiology
;
67.100.01
Milk and milk products in general
|
| ISO 11866-2:1997 |
Milk and milk products — Enumeration of presumptive Escherichia coli — Part 2: Most probable number technique using 4-methylumbelliferyl-beta-D-glucuronide (MUG) |
Describes a combined method for the enumeration of presumptive Escherichia coli and of presumptive coliforms by means of a culture technique involving a liquid medium with 4-methylumbelliferyl-ß-D-glucuronide (MUG), calculation of the number of Escherichia coli per gram or per millilitre by the most probable number (MPN) technique after incubation at 30 °C.
|
Withdrawn |
1997-02 |
Edition : 1 |
Number of pages : 10 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 11866-2:2005 | IDF 170-2:2005 |
Milk and milk products — Enumeration of presumptive Escherichia coli — Part 2: Colony-count technique at 44 degrees C using membranes |
ISO 11866-2|IDF 170-2:2005 specifies a method for the enumeration of presumptive Escherichia coli by means of a colony-count technique at 44 °C.
The method is applicable to milk, liquid milk products, dried milk, dried sweet whey, dried buttermilk, lactose, acid casein, lactic casein and rennet casein, caseinate and dried acid whey, cheese and processed cheese, butter, frozen milk products (including edible ices), and custard, desserts and cream.
The method specified in ISO 11866-2|IDF 170-2:2005 is the preferred method for samples in which comparatively large numbers of presumptive Escherichia coli (more than 100 per gram or 10 per millilitre) are suspected.
|
Published |
2005-12 |
Edition : 2 |
Number of pages : 9 |
Technical Committee |
07.100.30
Food microbiology
;
67.100.01
Milk and milk products in general
|
| ISO 11866-3:1997 |
Milk and milk products — Enumeration of presumptive Escherichia coli — Part 3: Colony-count technique at 44 degrees C using membranes |
Describes a method for the enumeration of presumptive Escherichia coli by means of a colony-count technique at 44 °C using membranes. Applicable to milk, liquid milk products, dried milk, butter, frozen milk products, cheese, cream, etc.
|
Withdrawn |
1997-02 |
Edition : 1 |
Number of pages : 9 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/CD 13136-1 |
Microbiology of the food chain — Detection, isolation and characterization of Shiga toxin-producing Escherichia coli (STEC) — Part 1: Horizontal method for the detection and isolation of Shiga toxin-producing Escherichia coli (STEC) |
This standard describes the detection and isolation of Shiga toxin-producing Escherichia coli (STEC). The procedure includes the detection by Real Time PCR of the major virulence genes of STEC, stx1 and stx2 (Reference [2]) in enrichment cultures. Isolation of STEC from the enrichment culture is attempted if one or both stx genes are detected in the screening.
This document is applicable to
— products intended for human consumption,
— products intended for animal feeding,
— environmental samples in the area of food and feed production, handling, and
— samples from the primary production stage.
|
Under development |
|
Edition : 1 |
|
Technical Committee |
07.100.30
Food microbiology
|
| ISO 15213:2003 |
Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of sulfite-reducing bacteria growing under anaerobic conditions |
ISO 15213:2003 specifies a horizontal method for the enumeration of sulfite-reducing bacteria growing under anaerobic conditions. It is applicable to
products intended for human consumption and the feeding of animals, andenvironmental samples in the area of food production and food handling.
|
Withdrawn |
2003-05 |
Edition : 1 |
Number of pages : 6 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/CD 13136-2 |
Microbiology of the food chain — Detection, isolation and characterization of Shiga toxin-producing Escherichia coli (STEC) — Part 2: Horizontal method for the characterization of Shiga toxin-producing Escherichia coli (STEC) isolates |
This document is applicable to pure cultures of STEC. The present standard describes the characterization of STEC strains, isolated from any source. In particular, the characterization of STEC strains described here regard:
- subtyping of the Stx-coding genes;
- determination of the presence of eae gene;
- determination of the presence of aggR gene;
- identification of the presence of genes associated to the following serogroups: O157, O26, O111, O103, O145, O121 and O45.
The full characterization of the isolated STEC strains is achieved by performing all the modules described here. The characterization scheme is not sequential, and the different modules can be implemented separately based on specific needs (e.g. Regulatory needs, Competent Authority’s requests, clients’ request). Alternative methods may be used, including Whole Genome Sequencing (WGS), provided these are verified according to the reference standard ISO 16140-2.
|
Under development |
|
Edition : 1 |
|
Technical Committee |
07.100.30
Food microbiology
|
| ISO/TS 13136:2012 |
Microbiology of food and animal feed — Real-time polymerase chain reaction (PCR)-based method for the detection of food-borne pathogens — Horizontal method for the detection of Shiga toxin-producing Escherichia coli (STEC) and the determination of O157, O111, O26, O103 and O145 serogroups |
ISO/TR 13136:2012 describes the identification of Shiga toxin-producing Escherichia coli (STEC) by means of the detection of the following genes: a) the major virulence genes of STEC, stx and eae; b) the genes associated with the serogroups O157, O111, O26, O103, and O145.
In any case, when one or both of the stx genes is/are detected, the isolation of the strain is attempted.
The isolation of STEC from samples positive for the presence of the genes specifying the serogroups in the scope of this method can be facilitated by using serogroup-specific enrichment techniques (e.g. immunomagnetic separation, IMS).
The protocol uses real-time PCR as the reference technology for detection of the virulence and serogroup-associated genes.
ISO/TR 13136:2012 is applicable to: 1) products intended for human consumption and the feeding of animals; 2) environmental samples in the area of food production and food handling; 3) environmental samples in the area of primary production.
|
Published |
2012-11 |
Edition : 1 |
Number of pages : 22 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 13307:2013 |
Microbiology of food and animal feed — Primary production stage — Sampling techniques |
ISO 13307:2013 specifies sampling techniques within the primary food-animal production stage, for detection or enumeration of viable microorganisms with particular reference to food-borne pathogens.
ISO 13307:2013 is not intended for use in diagnosis of animal disease.
|
Published |
2013-03 |
Edition : 1 |
Number of pages : 16 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 13559:2002 | IDF 153:2002 |
Butter, fermented milks and fresh cheese — Enumeration of contaminating microorganisms — Colony-count technique at 30 degrees C |
ISO 13559|IDF 153:2002 specifies a method for the enumeration of contaminating microorganisms by means of the colony-count technique at 30 °C. The method is applicable to butter, fermented milks and fresh cheese.
|
Published |
2002-11 |
Edition : 1 |
Number of pages : 7 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 13681:1995 |
Meat and meat products — Enumeration of yeasts and moulds — Colony-count technique |
Describes a method for the enumeration of yeasts and moulds of meat and meat products, including poultry, by means of the colony-count technique after aerobic incubation between 20 ° and 25 °C for 3, 4 or 5 days.
|
Withdrawn |
1995-12 |
Edition : 1 |
Number of pages : 6 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 13720:1995 |
Meat and meat products — Enumeration of Pseudomonas spp. |
Describes a method for the enumeration of Pseudomonas spp. in meat and meat products, including poultry, by means of a colony-count technique after aerobic incubation at 25 °C for 48 h.
|
Withdrawn |
1995-12 |
Edition : 1 |
Number of pages : 7 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 13720:2010 |
Meat and meat products — Enumeration of presumptive Pseudomonas spp. |
ISO 13720:2010 specifies a method for the enumeration of presumptive Pseudomonas spp. present in meat and meat products, including poultry.
|
Published |
2010-08 |
Edition : 2 |
Number of pages : 7 |
Technical Committee |
07.100.30
Food microbiology
;
67.120.10
Meat and meat products
|
| ISO 13721:1995 |
Meat and meat products — Enumeration of lactic acid bacteria — Colony-count technique at 30 degrees C |
|
Withdrawn |
1995-12 |
Edition : 1 |
Number of pages : 5 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 13721:1995/Cor 1:1996 |
Meat and meat products — Enumeration of lactic acid bacteria — Colony-count technique at 30 degrees C — Technical Corrigendum 1 |
|
Withdrawn |
1996-06 |
Edition : 1 |
Number of pages : 1 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 13722:1996 |
Meat and meat products — Enumeration of Brochothrix thermosphacta — Colony-count technique |
Describes a method for the enumeration of viable Brochothrix thermosphacta in meat and meat products, including poultry, by means of a colony-count technique.
|
Withdrawn |
1996-12 |
Edition : 1 |
Number of pages : 8 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 13722:2017 |
Microbiology of the food chain — Enumeration of Brochothrix spp. — Colony-count technique |
ISO 13722:2017 specifies a method for the enumeration of viable Brochothrix spp. by means of a colony-count technique.
ISO 13722:2017 is especially applicable to meat and meat products, but is also suitable for the examination of the following:
- other products intended for human consumption,
- products intended for animal feeding,
- environmental samples in the area of food and feed production, handling, and
- samples from the primary production stage.
|
Published |
2017-07 |
Edition : 2 |
Number of pages : 9 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 13969:2003 | IDF 183:2003 |
Milk and milk products — Guidelines for a standardized description of microbial inhibitor tests |
ISO 13969|IDF 183:2003 gives guidance for a standardized description of microbial inhibitor tests for milk and milk products. It is intended to give a framework and basis for the evaluation/validation of microbial inhibitor tests, allowing the comparison of data obtained from different tests and experimental studies.
|
Published |
2003-10 |
Edition : 1 |
Number of pages : 13 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 14461-1:2005 | IDF 169-1:2005 |
Milk and milk products — Quality control in microbiological laboratories — Part 1: Analyst performance assessment for colony counts |
ISO 14461-1|IDF 169-1:2005 describes a procedure for testing the performance of the colony-count technique within a laboratory by establishing the within-laboratory variability of its technique and identifying those steps that are associated with excessive variability.
The procedure is also suitable for checking the proper observance of Good Laboratory Practice (GLP), which may be a prerequisite for participation in interlaboratory tests of colony-count methods.
|
Published |
2005-05 |
Edition : 1 |
Number of pages : 34 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 14461-2:2005 | IDF 169-2:2005 |
Milk and milk products — Quality control in microbiological laboratories — Part 2: Determination of the reliability of colony counts of parallel plates and subsequent dilution steps |
ISO 14461-2|IDF 169-2:2005 describes a routine procedure for the evaluation of results of the enumeration of microorganisms using colony-count methods with subsequent 10-fold dilution steps and one plate or two parallel plates within each dilution step.
This routine procedure is applied regularly in each laboratory performing colony counts. It provides criteria for the acceptability of differences between the results from parallel plates and subsequent dilution steps, as follows.
The results (colony counts) obtained from parallel plates are compared with tabulated limits for given colony counts. If these limits are exceeded, a technical problem when performing the parallel determinations may be indicated.
The results (sums of colony counts) of two parallel plates of two subsequent 10-fold dilution steps are compared with tabulated limits for given sums of colony counts. If these limits are exceeded, a technical problem when performing the dilutions may be indicated.
If the limits mentioned above are exceeded in more cases than expected, this indicates that the test procedure lacks reliability.
|
Published |
2005-04 |
Edition : 1 |
Number of pages : 18 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 15174:2002 | IDF 176: 2002 |
Milk and milk products — Microbial coagulants — Determination of total milk-clotting activity |
ISO 15174|IDF 176:2002 describes a method to compare the total milk-clotting activity of a microbial coagulant sample with the milk-clotting activity of an international microbial coagulant reference standard on a standard milk substrate prepared with a calcium chloride solution containing 0,5 g/l of calcium chloride (pH approximately 6,5).
|
Withdrawn |
2002-09 |
Edition : 1 |
Number of pages : 8 |
Technical Committee |
07.100.30
Food microbiology
;
67.100.10
Milk and processed milk products
|
| ISO 15174:2012 | IDF 176:2012 |
Milk and milk products — Microbial coagulants — Determination of total milk-clotting activity |
ISO 15174:2012 specifies a method for comparison of the total milk-clotting activity of a microbial coagulant sample with the milk-clotting activity of an international microbial coagulant reference standard on a standard milk substrate prepared with a calcium chloride solution of concentration 0,5 g/l (pH ~6,5).
|
Published |
2012-05 |
Edition : 2 |
Number of pages : 9 |
Technical Committee |
07.100.30
Food microbiology
;
67.100.10
Milk and processed milk products
|
| ISO 15213-1:2023 |
Microbiology of the food chain — Horizontal method for the detection and enumeration of Clostridium spp. — Part 1: Enumeration of sulfite-reducing Clostridium spp. by colony-count technique |
This document specifies the enumeration of sulfite-reducing Clostridium spp. by the colony-count technique.
This document is applicable to:
— products intended for human consumption;
— products for feeding animals;
— environmental samples in the area of food and feed production and handling;
— samples from the primary production stage.
NOTE This method has been validated in an interlaboratory study for the following food categories:
— ready-to-eat, ready-to-reheat meat products;
— eggs and egg products (derivates);
— processed fruits and vegetables;
— infant formula and infant cereals;
— multi-component foods or meal components.
It has also been validated for the following other categories:
— pet food and animal feed;
— environmental samples (food or feed production).
As this method has been validated for at least five food categories, this method is applicable for a broad range of food. For detailed information on the validation, see Clause 11 and Annex C. Since the method is not commonly used for samples in the primary production stage, this category was not included in the interlaboratory study. Therefore, no performance characteristics were obtained for this category.
This horizontal method was originally developed for the examination of all samples belonging to the food chain. Based on the information available at the time of publication of this document, this method is considered to be fully suited to the examination of all samples belonging to the food chain. However, because of the large variety of products in the food chain, it is possible that this horizontal method is not appropriate in every detail for all products. Nevertheless, it is expected that the required modifications are minimized so that they do not result in a significant deviation from this horizontal method.
This technique is suitable for, but not limited to, the enumeration of microorganisms in test samples with a minimum of 10 colonies counted on a plate. This corresponds to a level of contamination that is expected to be higher than 10 cfu/ml for liquid samples or higher than 100 cfu/g for solid samples.
|
Published |
2023-01 |
Edition : 1 |
Number of pages : 21 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 15216-1:2017 |
Microbiology of the food chain — Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR — Part 1: Method for quantification |
ISO 15216-1:2017 specifies a method for the quantification of levels of HAV and norovirus genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs (soft fruit, leaf, stem and bulb vegetables, bottled water, BMS) or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR.
This method is not validated for detection of the target viruses in other foodstuffs (including multi-component foodstuffs), or any other matrices, nor for the detection of other viruses in foodstuffs, food surfaces or other matrices.
|
Published |
2017-03 |
Edition : 1 |
Number of pages : 48 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/TS 15216-1:2013 |
Microbiology of food and animal feed — Horizontal method for determination of hepatitis A virus and norovirus in food using real-time RT-PCR — Part 1: Method for quantification |
ISO/TS 15216-1:2013 describes a method for quantification of levels of HAV and NoV genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR.
This approach is also relevant for detection of the target viruses on fomites, or of other human viruses in foodstuffs, on food surfaces or on fomites following appropriate validation and using target-specific primer and probe sets.
|
Withdrawn |
2013-03 |
Edition : 1 |
Number of pages : 31 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 15216-1:2017/Amd 1:2021 |
Microbiology of the food chain — Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR — Part 1: Method for quantification — Amendment 1 |
|
Published |
2021-03 |
Edition : 1 |
Number of pages : 3 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 15216-2:2019 |
Microbiology of the food chain — Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR — Part 2: Method for detection |
This document specifies a method for detection of hepatitis A virus (HAV) and norovirus genogroups I (GI) and II (GII), from test samples of foodstuffs [(soft fruit, leaf, stem and bulb vegetables, bottled water, bivalve molluscan shellfish (BMS)] or surfaces using real-time RT-PCR.
This method is not validated for detection of the target viruses in other foodstuffs (including multi-component foodstuffs), or any other matrices, nor for the detection of other viruses in foodstuffs, surfaces or other matrices.
|
Published |
2019-07 |
Edition : 1 |
Number of pages : 40 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/TS 15216-2:2013 |
Microbiology of food and animal feed — Horizontal method for determination of hepatitis A virus and norovirus in food using real-time RT-PCR — Part 2: Method for qualitative detection |
ISO/TS 15216-2:2013 describes a method for qualitative detection of HAV and NoV genogroups I (GI) and II (GII), from test samples of foodstuffs or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR.
This approach is also relevant for detection of the target viruses on fomites, or of other human viruses in foodstuffs, on food surfaces or on fomites following appropriate validation and using target-specific primer and probe sets.
|
Withdrawn |
2013-03 |
Edition : 1 |
Number of pages : 27 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 16140-1:2016 |
Microbiology of the food chain — Method validation — Part 1: Vocabulary |
ISO 16140-1:2016 defines general terms and definitions relating to method validation of microbiology in the food chain.
It is applicable to the validation of methods for the analysis (detection or quantification) of microorganisms in
- products intended for human consumption,
- products intended for animal feeding,
- environmental samples in the area of food and feed production, handling, and
- samples from the primary production stage.
|
Published |
2016-06 |
Edition : 1 |
Number of pages : 12 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 16140-2:2016 |
Microbiology of the food chain — Method validation — Part 2: Protocol for the validation of alternative (proprietary) methods against a reference method |
ISO 16140-2:2016 specifies the general principle and the technical protocol for the validation of alternative, mostly proprietary, methods for microbiology in the food chain. Validation studies according to ISO 16140-2:2016 are intended to be performed by organizations involved in method validation.
It is applicable to the validation of methods for the analysis (detection or quantification) of microorganisms in
- products intended for human consumption,
- products intended for animal feeding,
- environmental samples in the area of food and feed production, handling, and
- samples from the primary production stage.
It is in particular applicable to bacteria and fungi. Some clauses of ISO 16140-2:2016 could be applicable to other (micro) organisms or their metabolites on a case-by-case-basis. In the future, guidance for other organisms (e.g. viruses and parasites) will be included in ISO 16140:2016 (all parts).
|
Published |
2016-06 |
Edition : 1 |
Number of pages : 66 |
Technical Committee |
07.100.30
Food microbiology
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| ISO 16140-2:2016/CD Amd 1 |
Microbiology of the food chain — Method validation — Part 2: Protocol for the validation of alternative (proprietary) methods against a reference method — Amendment 1 |
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Under development |
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Edition : 1 |
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Technical Committee |
07.100.30
Food microbiology
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| ISO 16140-3:2021 |
Microbiology of the food chain — Method validation — Part 3: Protocol for the verification of reference methods and validated alternative methods in a single laboratory |
This document specifies the protocol for the verification of reference methods and validated alternative methods for implementation in the user laboratory.
This document is applicable to the verification of methods used for the analysis (detection and/or quantification), confirmation and typing of microorganisms in:
— products intended for human consumption;
— products intended for animal feeding;
— environmental samples in the area of food and feed production, handling;
— samples from the primary production stage.
This document is, in particular, applicable to bacteria and fungi. Some clauses can be applicable to other (micro)organisms or their metabolites, to be determined on a case-by-case basis.
The technical protocols for the verification of validated qualitative methods and validated quantitative methods are described in Clauses 5 and 6. The technical protocol for the verification of validated alternative confirmation and typing methods is described in Clause 7. The protocols for the verification of non-validated reference methods are described in Annex F.
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Published |
2021-01 |
Edition : 1 |
Number of pages : 70 |
Technical Committee |
07.100.30
Food microbiology
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| ISO 16140-4:2020 |
Microbiology of the food chain — Method validation — Part 4: Protocol for method validation in a single laboratory |
This document specifies the general principles and the technical protocols for single-laboratory validation of methods for microbiology in the food chain. The protocols in this document only validate the method for the laboratory conducting the study.
This document is applicable to single-laboratory validation of:
— methods used in the analysis (detection or quantification) of microorganisms in:
— products intended for human consumption;
— products intended for animal feeding;
— environmental samples in the area of food and feed production, handling;
— samples from the primary production stage;
— methods for the confirmation or typing of microorganisms. This validation will replace only the confirmation or typing procedure of a specified method (see Annex G).
This document is, in particular, applicable to bacteria and fungi. Some clauses can be applicable to other (micro)organisms or their metabolites, to be determined on a case-by-case basis.
Single-laboratory validation is required if an interlaboratory validation in accordance with ISO 16140-2 is not appropriate. Possible applications are:
— validation of an in-house method;
— method evaluation study in the validation process of a reference method in accordance with ISO 17468;
— extension of the scope of an ISO 16140-2 validated method, e.g. category extension or test portion size;
— modifications of existing methods.
Single-laboratory validation is the second step in the standardization of a reference method (see ISO 17468). It is only applicable to methods that are fully specified with regard to all relevant parameters (including tolerances on temperatures and specifications on culture media) and that have already been optimized.
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Published |
2020-07 |
Edition : 1 |
Number of pages : 46 |
Technical Committee |
07.100.30
Food microbiology
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| ISO 16140-4:2020/CD Amd 1 |
Microbiology of the food chain — Method validation — Part 4: Protocol for method validation in a single laboratory — Amendment 1: Annex H - Validation of larger test portion size for qualitative methods |
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Under development |
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Edition : 1 |
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Technical Committee |
07.100.30
Food microbiology
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