| Name |
Description |
Abstract |
Status |
Publication date |
Edition |
Number of pages |
Technical committee |
ICS |
| ISO 20635:2018 |
Infant formula and adult nutritionals — Determination of vitamin C by (ultra) high performance liquid chromatography with ultraviolet detection ((U)HPLC-UV) |
This document specifies a method for the determination of vitamin C (L-ascorbic acid) present in all forms of infant and adult formulas (powders, ready-to-feed liquids and liquid concentrates), using (ultra) high performance liquid chromatography with ultraviolet detection (U)HPLC-UV. The application range runs from 2,5 mg/100 g (limit of quantification) to 50 mg/100 g expressed in the product as consumed. The method is able to distinguish between D-ascorbic acid (isoascorbic- or erythorbic acid) and L-ascorbic acid.
|
Published |
2018-07 |
Edition : 1 |
Number of pages : 13 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO 20636:2018 |
Infant formula and adult nutritionals — Determination of vitamin D by liquid chromatography-mass spectrometry |
This document specifies a method for the quantitative determination of vitamin D2 and/or vitamin D3 in infant formula, and adult nutritionals in solid (i.e. powders) or liquid (i.e. ready-to-feed liquids and liquid concentrates) forms using liquid chromatography-mass spectrometry. The application range runs from 0,15 µg/100 g (limit of quantification) to 59 µg/100 g for vitamin D2 and from 0,25 µg/100 g to 65 µg/100 g for vitamin D3.
|
Published |
2018-07 |
Edition : 1 |
Number of pages : 23 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO 20637:2015 |
Infant formula and adult nutritionals — Determination of myo-inositol by liquid chromatography and pulsed amperometry |
ISO 20637:2015 specifies a method for the determination of myo-inositol (free or free plus bound as phosphatidylinositol) in infant formula and adult nutritionals using liquid chromatography and pulsed amperometry with column switching.
|
Published |
2015-11 |
Edition : 1 |
Number of pages : 15 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO 20638:2015 |
Infant formula — Determination of nucleotides by liquid chromatography |
ISO 20638:2015 specifies a method for the quantitative determination of 5′-mononucleotides in infant formula in solid (i.e. powders) or liquid (i.e. ready-to-feed liquids and liquid concentrates) forms using liquid chromatography.
|
Published |
2015-11 |
Edition : 1 |
Number of pages : 14 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO 20639:2015 |
Infant formula and adult nutritionals — Determination of pantothenic acid by ultra high performance liquid chromatography and tandem mass spectrometry method (UHPLC-MS/MS) |
ISO 20639:2015 specifies a method for the quantitative determination of pantothenic acid, excluding bound forms, in infant formula and adult nutritionals (i.e. powders) using ultra high performance liquid chromatography and tandem mass spectrometry method (UHPLC-MS/MS).
|
Published |
2015-11 |
Edition : 1 |
Number of pages : 8 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO 20647:2015 | IDF 234:2015 |
Infant formula and adult nutritionals — Determination of total iodine — Inductively coupled plasma mass spectrometry (ICP-MS) |
ISO 20647:2015 specifies a method for the quantitative determination of total iodine in infant formula and adult nutritional formula.[1] The method is applicable to the measurement of total iodine in infant formula and adult nutritional formula from 0,5 µg/100g to 1 500 µg/100g reconstituted final product and for ready-to-feed products from 2,5 µg/100 g to 1 000 µg/100 g using ICP-MS.
Using various infant formula and adult nutritional products, the method was subjected to an interlaboratory study. Levels obtained ranged from 3,47 µg/100 g to 124 µg/100 g. For all precision data related to the interlaboratory study, see Table A.1 located in Annex A.
|
Published |
2015-11 |
Edition : 1 |
Number of pages : 13 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO/DIS 20647 | IDF 234 |
Infant formula and adult nutritionals — Determination of total iodine — Inductively coupled plasma mass spectrometry (ICP-MS) |
ISO 20647:2015 specifies a method for the quantitative determination of total iodine in infant formula and adult nutritional formula.[1] The method is applicable to the measurement of total iodine in infant formula and adult nutritional formula from 0,5 µg/100g to 1 500 µg/100g reconstituted final product and for ready-to-feed products from 2,5 µg/100 g to 1 000 µg/100 g using ICP-MS.
Using various infant formula and adult nutritional products, the method was subjected to an interlaboratory study. Levels obtained ranged from 3,47 µg/100 g to 124 µg/100 g. For all precision data related to the interlaboratory study, see Table A.1 located in Annex A.
|
Under development |
|
Edition : 2 |
|
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO 20649:2015 | IDF 235:2015 |
Infant formula and adult nutritionals — Determination of chromium, selenium and molybdenum — Inductively coupled plasma mass spectrometry (ICP-MS) |
ISO 20649:2015 specifies a method for the quantitative determination of chromium, selenium and molybdenum in infant formula and adult nutritional formula.[1] The method has been validated in an interlaboratory study on seven different matrices.
|
Published |
2015-11 |
Edition : 1 |
Number of pages : 8 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO 21569:2005/Amd 1:2013 |
Foodstuffs — Methods of analysis for the detection of genetically modified organisms and derived products — Qualitative nucleic acid based methods — Amendment 1 |
|
Published |
2013-04 |
Edition : 1 |
Number of pages : 87 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO/DIS 20649 | IDF 235 |
Infant formula and adult nutritionals — Determination of chromium, selenium and molybdenum — Inductively coupled plasma mass spectrometry (ICP-MS) |
ISO 20649:2015 specifies a method for the quantitative determination of chromium, selenium and molybdenum in infant formula and adult nutritional formula.[1] The method has been validated in an interlaboratory study on seven different matrices.
|
Under development |
|
Edition : 2 |
|
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO 20813:2019 |
Molecular biomarker analysis — Methods of analysis for the detection and identification of animal species in foods and food products (nucleic acid-based methods) — General requirements and definitions |
This document specifies minimum requirements of performance characteristics for the detection of nucleic acid sequences (DNA) by molecular methods, such as the polymerase chain reaction (PCR), including different post-PCR detection methods, real-time PCR, single and/or multiple probe-based detection techniques as well as the combination of such methods.
The document is applicable to the detection, identification and quantification of DNA from animal species of higher and lower taxonomic groups in foodstuffs, and the validation of applicable methods.
It is applicable to mammals, birds, reptiles, amphibians, fishes, molluscs, crustaceans and insects. Typical examples for each are listed in Annex A.
|
Published |
2019-05 |
Edition : 1 |
Number of pages : 27 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO/TS 21098:2005 |
Foodstuffs — Nucleic acid based methods of analysis of genetically modified organisms and derived products — Information to be supplied and procedure for the addition of methods to ISO 21569, ISO 21570 or ISO 21571 |
ISO/TS 21098:2005 defines the principles and specifies the nature of the information to be supplied for acceptance of a method as an annex to ISO 21569, ISO 21570 or ISO 21571. It also specifies the process for adding, amending and retaining methods annexed to these standards. ISO/TS 21098:2005 is necessary in order to attain consistency in methods that are to be employed as part of the standards. It does not cover the specifics of the development of a method or laboratory set-up.
|
Withdrawn |
2005-09 |
Edition : 1 |
Number of pages : 18 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO 21446:2019 |
Infant formula and adult nutritionals — Determination of trans and total (cis + trans) vitamin K1 content — Normal phase HPLC |
This document specifies a method for the quantitative determination of trans and total (cis + trans) vitamin K1 in infant, pediatric and adult nutritionals using normal phase (NP) high-performance liquid chromatography (HPLC) with post-column reduction and fluorescence detection. The method demonstrated good linearity over a standard range of ~2 μg/l to 80 μg/l trans vitamin K1, and the limit of quantification (LOQ) was estimated to be 0,4 μg/l for standards and 0,09 μg/100 g ready to feed (RTF) for samples assuming 4 grams of sample are diluted to 10 ml.
|
Published |
2019-01 |
Edition : 1 |
Number of pages : 20 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO 21468:2020 |
Infant formula and adult nutritionals — Determination of free and total choline and free and total carnitine — Liquid chromatography tandem mass spectrometry (HPLC-MS/MS) |
This document specifies a method for the determination of total or free choline and carnitine in infant formula and adult nutritionals by liquid chromatography and tandem mass spectrometry (HPLC-MS/MS).
|
Published |
2020-10 |
Edition : 1 |
Number of pages : 16 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO 21470:2020 |
Infant formula and adult nutritionals — Simultaneous determination of total vitamins B1, B2, B3 and B6 — Enzymatic digestion and LC-MS/MS |
This document specifies a method for the simultaneous quantitative determination of four water-soluble vitamins in infant formula and related nutritional products, including relevant forms of vitamins B1, B2, B3 and B6 by enzymatic digestion and UHPLC-MS/MS. This document is not intended to be used on products where vitamins have not been added.
|
Published |
2020-11 |
Edition : 1 |
Number of pages : 31 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO/TS 21569-2:2012 |
Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 2: Construct-specific real-time PCR method for detection of event FP967 in linseed and linseed products |
This method describes a procedure for the detection of a DNA sequence present in a genetically modified linseed (Linum usitatissimum) line (event FP967, also named as "CDC Triffid"). For this purpose, extracted DNA is used in a real-time PCR and the genetic modification (GM) is specifically detected by amplification of a 105 bp DNA sequence representing the transition between the nopalin synthase gene terminator (Tnos) from Agrobacterium tumefaciens and the dihydrofolate reductase gene (dfrA1) from a Class 1 integron of Escherichia coli.
The method described is applicable for the analysis of DNA extracted from foodstuffs. It may also be suitable for the analysis of DNA extracted from other products such as feedstuffs and seeds. The application of this method requires the extraction of an adequate amount of amplifiable DNA from the relevant matrix for the purpose of analysis.
|
Withdrawn |
2012-09 |
Edition : 1 |
Number of pages : 9 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO 24276:2006/Amd 1:2013 |
Foodstuffs — Methods of analysis for the detection of genetically modified organisms and derived products — General requirements and definitions — Amendment 1 |
|
Published |
2013-04 |
Edition : 1 |
Number of pages : 11 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO/TS 21569-2:2021 |
Molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 2: Construct-specific real-time PCR method for detection of event FP967 in linseed and linseed products |
This document specifies a procedure for the detection of a DNA sequence present in a genetically modified linseed (Linum usitatissimum) line (event FP967, also named as “CDC Triffid”). For this purpose, extracted DNA is used in a real-time PCR and the genetic modification (GM) is specifically detected by amplification of a 105 bp DNA sequence representing the transition between the nopaline synthase gene terminator (Tnos) from Agrobacterium tumefaciens and the dihydrofolate reductase gene (dfrA1) from a Class 1 integron of Escherichia coli.
The method described is applicable for the analysis of DNA extracted from foodstuffs. It can also be suitable for the analysis of DNA extracted from other products such as feedstuffs and seeds. The application of this method requires the extraction of an adequate amount of amplifiable DNA from the relevant matrix for the purpose of analysis.
|
Published |
2021-07 |
Edition : 2 |
Number of pages : 10 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO/TS 21569-3:2015 |
Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 3: Construct-specific real-time PCR method for detection of P35S-pat-sequence for screening genetically modified organisms |
ISO/TS 21569-3:2015 describes a procedure for the detection of the DNA transition sequence between the 35S promotor (P35S) from Cauliflower mosaic virus and a modified phoshinothricin-acetyltransferase gene (pat) from Streptomyces viridochromogenes.
ISO/TS 21569-3:2015 is applicable for the analysis of DNA extracted from foodstuffs. It may also be suitable for the analysis of DNA extracted from other products such as feedstuffs and seeds.
|
Withdrawn |
2015-02 |
Edition : 1 |
Number of pages : 9 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO/TS 21569-3:2020 |
Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 3: Construct-specific real-time PCR method for detection of P35S-pat-sequence for screening for genetically modified organisms |
This document describes a procedure for the detection of the DNA transition sequence between the 35S promotor (P35S) from Cauliflower mosaic virus and a modified phoshinothricin-acetyltransferase gene (pat) from Streptomyces viridochromogenes. The P35S-pat construct is frequently found in genetically modified plants with tolerance for phosphinothricin-containing herbicides. The P35S-pat construct specific method is based on a real-time PCR and can be used for qualitative and quantitative screening purposes. For identification and quantification of a specific event, a follow-up analysis can be carried out.
This document is applicable to the analysis of DNA extracted from foodstuffs. It can also be suitable for the analysis of DNA extracted from other products such as feedstuffs and seeds. The application of this method requires the extraction of an adequate quantity and quality of amplifiable DNA from the relevant matrix.
|
Published |
2020-06 |
Edition : 2 |
Number of pages : 14 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO/TS 21569-4:2016 |
Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 4: Real-time PCR based screening methods for the detection of the P-nos and P-nos-nptII DNA sequences |
ISO/TS 21569-4:2016 specifies a procedure for the detection of a DNA sequence of the promoter region of the nopaline synthase gene (P-nos) from Agrobacterium tumefaciens and a procedure for the detection of the DNA transition sequence between P-nos and the neomycin-phosphotransferase gene (nptII) from the Tn5 transposon of Escherichia coli K12. The nos-promoter and the P-nos-nptII-construct are frequently found in genetically modified plants. The P-nos and P-nos-nptII specific methods are based on real-time PCR and can be used for qualitative screening purposes. For identification and quantification of a specific genetically modified plant (event) a follow-up analysis has to be carried out.
The methods described are applicable for the analysis of DNA extracted from foodstuffs. They may also be suitable for the analysis of DNA extracted from other products such as feedstuffs and seeds. The application of these methods requires the extraction of an adequate amount of amplifiable DNA from the relevant matrix.
The DNA sequence amplified by the P-nos element-specific method can be detected in samples which contain DNA of the naturally occurring Ti-plasmid of A. tumefaciens. For this reason, it is necessary to confirm a positive screening result. Further analyses are required using construct-specific or event specific methods.
|
Published |
2016-11 |
Edition : 1 |
Number of pages : 10 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO/TS 21569-5:2016 |
Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 5: Real-time PCR based screening method for the detection of the FMV promoter (P-FMV) DNA sequence |
ISO/TS 21569-5:2016 specifies a procedure for the detection of a DNA sequence used in genetically modified (GM) plants by means of a real-time PCR (polymerase chain reaction). The method detects a 78 base pairs long segment of the Figwort mosaic virus 34S promoter DNA sequence. This segment in some GM plants is indicated as FMV promoter (P-FMV) and in other GM plants as FMV enhancer (E-FMV).
The method was developed and validated for the analysis of DNA extracted from foodstuffs. It may be suitable also for analysis of other products such as feedstuffs and seeds. The procedure requires the extraction of an adequate quantity and quality of amplifiable DNA from the test sample.
The DNA sequence amplified by the P-FMV element-specific method can be detected in samples which contain DNA of the naturally occurring Figwort mosaic virus. For this reason, it is necessary to confirm a positive screening result. Further analyses are required using construct-specific or event specific methods.
|
Published |
2016-11 |
Edition : 1 |
Number of pages : 10 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO/TS 21569-6:2016 |
Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 6: Real-time PCR based screening methods for the detection of cry1Ab/Ac and Pubi-cry DNA sequences |
ISO/TS 21569-6:2016 specifies a procedure for the detection of a DNA sequence of the modified cry1Ab/Ac gene and a procedure for the detection of the DNA transition sequence between the maize ubiquitin promoter (Pubi) and the cry1Ab/Ac gene. The modified cry1Ab/Ac gene and the Pubi-cry construct are frequently found in genetically modified Bt plants. Both detection methods are based on real-time PCR and can be used for qualitative screening purposes. For identification and quantification of a specific genetically modified plant (event) a follow-up analysis has to be carried out.
ISO/TS 21569-6:2016 is applicable for the analysis of DNA extracted from foodstuffs. It may also be suitable for the analysis of DNA extracted from other products such as feedstuffs and seeds. The application of these methods requires the extraction of an adequate amount of amplifiable DNA from the relevant matrix.
|
Published |
2016-11 |
Edition : 1 |
Number of pages : 10 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO/TS 21569-7:2022 |
Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 7: Real-time PCR based methods for the detection of CaMV and Agrobacterium Ti-plasmid derived DNA sequences |
This document specifies a procedure for the detection of a DNA sequence of the open reading frame five (ORF V) from cauliflower mosaic virus (CaMV) and a procedure for the detection of the DNA sequence of the nopaline synthase (nos) gene from tumour-inducing (Ti) plasmids of phytopathogenic Rhizobium radiobacter (formerly named Agrobacterium tumefaciens). The procedures can be used in the context of screening for genetically modified crop/plants and their derived products to further clarify a positive PCR result for a specific promoter or terminator of CaMV (P-35S, T-35S), or both, and the nos gene (P-nos, T-nos), respectively.
The methods specified in this document will detect and identify naturally occurring CaMV or Rhizobium radiobacter (Ti plasmid) DNA, or both, if present in the sample in the absence of a genetically modified plant event containing the specified target sequences.
Both methods are based on the real-time polymerase chain reaction (PCR) and are applicable for the analysis of DNA extracted from foodstuffs and other products such as feedstuffs and seeds/grains. The application of the methods requires the extraction of an adequate amount of amplifiable DNA from the relevant matrix.
With appropriate calibration material, the CaMV ORF V or nos copy number, or both, can be estimated and compared, respectively, with the estimated copy number for the promoter (P-35S, P-nos) or the terminator (T-35S, T-nos) sequences, or both. Thereby, conclusions are possible about the presence of an unknown genetically modified organism (GMO) in addition to any detected CaMV DNA or Rhizobium radiobacter Ti plasmid DNA, or both, in a test sample.
|
Published |
2022-12 |
Edition : 1 |
Number of pages : 12 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO/CD TS 21569-8 |
Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 8: DNA extraction from alfalfa seeds and real-time PCR based event-specific detection methods for genetically modified alfalfa lines J101, J163 and KK179 |
This Technical Specification describes procedures for DNA extraction from alfalfa seeds and for the event-specific detection of the alfalfa lines J101, J163 and KK179.
The detection methods are based on real-time PCR and are targeting the DNA transition sequences between the alfalfa genome and the respective integrated gene construct. The methods can be applied for direct event-specific identification or as a follow-up analysis, if sequences encoding the promoter of the figwort mosaic virus (P-FMV), the terminator of the nopaline synthase gene from Rhizobium radiobacter (T-nos), or the construct CTP2-CP4-EPSPS (herbicide tolerance) were detected by screening analyses of alfalfa samples.
In this part of ISO 21569 the DNA extraction method was validated using ground alfalfa seeds and the PCR methods using DNA extracted from seeds. The PCR methods may also be used for the analysis of other matrices (e.g. feed- and foodstuffs). The application of the PCR methods require the extraction of an adequate amount of amplifiable DNA from the relevant matrix.
|
Under development |
|
Edition : 1 |
|
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO/CD TS 21569-9 |
Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 9: Construct-specific real-time PCR based screening method for the detection of the P-35S-nptII DNA-sequences |
This proposed Technical Specification describes a procedure for the detection of the DNA transition sequence between the 35S promoter region from Cauliflower mosaic virus (P-35S) and the neomycin-phosphotransferase gene (nptII) from the Tn5 transposon of Escherichia coli. The P-35S-nptII construct which confers resistance to neomycin/kanamycin antibiotics is frequently found in genetically modified plants.
The detection method is based on real-time PCR and can be used for qualitative screening purposes. For identification and quantification of a specific genetically modified plant (event) a follow-up analysis has to be carried out.
This part of ISO 21569 is applicable for the analysis of DNA extracted from foodstuffs. It may also be suitable for the analysis of DNA extracted from other products such as feedstuffs and seeds. The application of this method requires the extraction of an adequate amount of amplifiable DNA from the relevant matrix.
|
Under development |
|
Edition : 1 |
|
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO 21569:2005 |
Foodstuffs — Methods of analysis for the detection of genetically modified organisms and derived products — Qualitative nucleic acid based methods |
ISO 21569:2005 describes the procedure to qualitatively detect genetically modified organisms (GMOs) and derived products by analysing the nucleic acids extracted from the sample under study. The main focus is on polymerase chain reaction (PCR) based amplification methods.
It gives general requirements for the specific detection and identification of target nucleic acid sequences (DNA) and for the confirmation of the identity of the amplified DNA sequence.
Guidelines, minimum requirements and performance criteria laid down in ISO 21569:2005 are intended to ensure that comparable, accurate and reproducible results are obtained in different laboratories.
ISO 21569:2005 has been established for food matrices, but could also be applied to other matrices (e.g. feed and plant samples from the environment).
Specific examples of methods are provided in Annexes A to D.
|
Published |
2005-06 |
Edition : 1 |
Number of pages : 69 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO 21570:2005 |
Foodstuffs — Methods of analysis for the detection of genetically modified organisms and derived products — Quantitative nucleic acid based methods |
ISO 21570:2005 provides the overall framework of quantitative methods for the detection of genetically modified organisms (GMO) in foodstuffs, using the polymerase chain reaction (PCR).
It defines general requirements for the specific amplification of DNA target sequences in order to quantify the relative GMO-derived DNA content and to confirm the identity of the amplified DNA sequence.
Guidelines, minimum requirements and performance criteria laid down in ISO 21570:2005 are intended to ensure that comparable, accurate and reproducible results are obtained in different laboratories.
ISO 21570:2005 has been established for food matrices, but is also applicable to other matrices, e.g. feed and plant samples from the environment.
|
Published |
2005-11 |
Edition : 1 |
Number of pages : 103 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO 21570:2005/Amd 1:2013 |
Foodstuffs — Methods of analysis for the detection of genetically modified organisms and derived products — Quantitative nucleic acid based methods — Amendment 1 |
|
Published |
2013-04 |
Edition : 1 |
Number of pages : 18 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO 21570:2005/Cor 1:2006 |
Foodstuffs — Methods of analysis for the detection of genetically modified organisms and derived products — Quantitative nucleic acid based methods — Technical Corrigendum 1 |
|
Published |
2006-12 |
Edition : 1 |
Number of pages : 2 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO 21571:2005 |
Foodstuffs — Methods of analysis for the detection of genetically modified organisms and derived products — Nucleic acid extraction |
ISO 21571:2005 provides general requirements and specific methods for DNA extraction/purification and quantification. These methods are described in Annexes A and B.
ISO 21571:2005 has been established for food matrices, but could also be applicable to other matrices, such as grains and feed.
It has been designed as an integral part of nucleic-acid-based analytical methods, in particular ISO 21569 on qualitative analytical methods, and ISO 21570 on quantitative analytical methods.
|
Published |
2005-02 |
Edition : 1 |
Number of pages : 43 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO 21571:2005/Amd 1:2013 |
Foodstuffs — Methods of analysis for the detection of genetically modified organisms and derived products — Nucleic acid extraction — Amendment 1 |
|
Published |
2013-03 |
Edition : 1 |
Number of pages : 10 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO 21572:2004 |
Foodstuffs — Methods for the detection of genetically modified organisms and derived products — Protein based methods |
ISO 21572:2004 provides general guidelines and performance criteria for methods for the detection and/or quantitation of specific proteins derived from genetically modified (GM) plant material in a specified matrix.
These general guidelines address existing antibody based methods. Methods other than those described in annex A may also detect the protein. The same criteria as outlined in this standard generally apply.
|
Withdrawn |
2004-03 |
Edition : 1 |
Number of pages : 21 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO 21572:2004/Cor 1:2005 |
Foodstuffs — Methods for the detection of genetically modified organisms and derived products — Protein based methods — Technical Corrigendum 1 |
|
Withdrawn |
2005-02 |
Edition : 1 |
Number of pages : 1 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO 21572:2013 |
Foodstuffs — Molecular biomarker analysis — Protein-based methods |
ISO 21572:2013 provides general guidelines and performance criteria for methods for the detection and/or quantification of specific proteins or protein(s) of interest [POI(s)] in a specified matrix.
These general guidelines address existing antibody based methods. Methods other than those described in Annex A or Annex B can also detect the POI. The same criteria as outlined in ISO 21572:2013 apply generally.
|
Withdrawn |
2013-02 |
Edition : 2 |
Number of pages : 29 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO 21572:2019 |
Foodstuffs — Molecular biomarker analysis — Immunochemical methods for the detection and quantification of proteins |
This document specifies performance criteria for immunochemical methods for the detection and/or quantification of a specific protein or protein(s) of interest [POI(s)] in a specified matrix.
The methods discussed are applicable to the analysis of proteins from a variety of sample types. Some uses for these methods include, but are not limited to, analysing proteins involved in crop and food production, food processing, food marketing, food safety, biotechnology or disease indexing.
|
Published |
2019-10 |
Edition : 3 |
Number of pages : 25 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO 6730:1992 |
Milk — Enumeration of colony-forming units of psychrotrophic micro-organisms — Colony-count technique at 6,5 degrees C |
Specifies the principle, the diluents and culture medium, the apparatus and glassware, the sampling and procedure, the expression of results, the repeatability and the test report.
|
Withdrawn |
1992-01 |
Edition : 1 |
Number of pages : 6 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 22579:2020 | IDF 241:2020 |
Infant formula and adult nutritionals — Determination of fructans — High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) after enzymatic treatment |
This document specifies a method for the determination of inulin-type fructans (including oligofructose, fructooligosaccharides) in infant formula and adult nutritionals (both powder and liquid) containing 0,03 g/100 g to 5,0 g/100 g of fructans in the product as prepared ready for consumption.
The method has been validated in a multi laboratory study[1] with reconstituted standard reference material (SRM), infant/adult nutritional formula at a level of 0,204 g/100 g, adult nutritionals ready-to-feed (RTF) at levels of 1,28 g/100 g and 2,67 g/100 g, infant formula RTF at a level of 0,300 g/100 g, reconstituted follow-up formula at levels of 0,209 g/100 g to 0,275 g/100 g, reconstituted infant formula at levels from 0,030 8 g/100 g to 0,264 g/100 g. During the single laboratory validation study[2], spike-recovery experiments were performed up to 5 g/100 g in reconstituted infant formula powders (milk-based, partially hydrolysed milk-based and soy-based), adult nutritional RTF and reconstituted adult nutritional powders.
|
Published |
2020-09 |
Edition : 1 |
Number of pages : 22 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO 22753:2021 |
Molecular biomarker analysis — Method for the statistical evaluation of analytical results obtained in testing sub-sampled groups of genetically modified seeds and grains — General requirements |
This document describes general requirements, procedures and performance criteria for evaluating the content of genetically modified (GM) seeds/grains in a lot by a group testing strategy that includes qualitative analysis of sub-sampled groups followed by statistical evaluation of the results.
This document is applicable to group testing strategy estimating the GM content on a percentage seed/grain basis for purity estimation, testing towards a given reject/accept criterion and for cases where seed/grain lots are carrying stacked events.
This document is not applicable to processed products.
NOTE Description of the use of group testing strategy are available in References [1], [7], [8], [18], [19] and [20].
|
Published |
2021-08 |
Edition : 1 |
Number of pages : 26 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO 22942-1:2022 |
Molecular biomarker analysis — Isothermal polymerase chain reaction (isoPCR) methods — Part 1: General requirements |
This document specifies general criteria for development, validation and use of nucleic acid analytical methods based on the isothermal polymerase chain reaction (isoPCR). It provides additional information and guidance for specific isoPCR technologies.
This document is applicable to food, feed, plant matrices and their propagules, plant pathogens, and animals in which amplification of a specific biomolecular target sequence is required.
|
Published |
2022-03 |
Edition : 1 |
Number of pages : 40 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO 22949-1:2021 |
Molecular biomarker analysis — Methods of analysis for the detection and identification of animal species in food and feed products (nucleotide sequencing-based methods) — Part 1: General requirements |
This document specifies general requirements for DNA sequencing method performance in the detection and identification of animal species in food and feed products. Performance requirements are limited to Sanger and next generation sequencing (NGS), including second and third generation sequencing, for analysis of single species products and multispecies products.
This document is applicable to DNA sequences for mammals, birds, fish, molluscs, crustaceans, amphibians, reptiles and insects, and to the validation of the applicable methods.
Methods for DNA species quantification are not considered under the scope of this document.
|
Published |
2021-10 |
Edition : 1 |
Number of pages : 21 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO 23443:2020 |
Infant formula and adult nutritionals — Determination of β-carotene, lycopene and lutein by reversed-phase ultra-high performance liquid chromatography (RP-UHPLC) |
This document specifies a method for the quantitative determination of β-carotene and lycopene in infant formula and adult nutritionals in solid (i.e. powders) or liquid (i.e. ready-to-feed liquids and liquid concentrates) forms using reversed-phase ultra-high performance liquid chromatography (RP-UHPLC) and UV-visible detection. The application range runs from 1 μg/100 g to 1 500 μg/100 g for lycopene and from 1 μg/100 g to 2 250 μg/100 g for β-carotene. Based on the single-laboratory validation, the limit of detection (LOD) was 0,1 μg/100 g and the limit of quantification (LOQ) was 0,3 μg/100 g for each carotenoid.
The method does not apply to materials that contain measurable levels of β-apo-8′-carotenal. The reproducibility data meets the requirements given in References [8] and [10].
Annex C specifies the determination of lutein. The reproducibility data does not meet the requirements given in Reference [9].
|
Published |
2020-07 |
Edition : 1 |
Number of pages : 32 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO 24276:2006 |
Foodstuffs — Methods of analysis for the detection of genetically modified organisms and derived products — General requirements and definitions |
ISO 24276:2006 specifies how to use the standards for sampling strategies (EN/TS 21568), nucleic acid extraction (ISO 21571), qualitative nucleic acid analysis (ISO 21569) and quantitative nucleic acid analysis (ISO 21570), and their relationship in the analysis of genetically modified organisms in foodstuffs, and contains general definitions, requirements and guidelines for laboratory set-up, method validation requirements, description of methods and test reports.
It has been established for food matrices, but could also be applied to other matrices (e.g. seeds, feed and plant samples from the environment).
|
Published |
2006-02 |
Edition : 1 |
Number of pages : 16 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO 24583:2022 |
Quantitative nuclear magnetic resonance spectroscopy — Purity determination of organic compounds used for foods and food products — General requirements for 1H NMR internal standard method |
This document specifies general requirements and performance criteria for the determination of purity of organic compounds through the application of solution state proton (1H) quantitative nuclear magnetic resonance (qNMR) spectroscopy using an internal standard method.
This document is applicable to bioactive compounds in functional foods, natural toxins, food additives and pesticides.
This document is applicable to the users pursuing metrological traceability of the measurement results.
|
Published |
2022-12 |
Edition : 1 |
Number of pages : 36 |
Technical Committee |
67.050
General methods of tests and analysis for food products
|
| ISO 2293:1976 |
Meat and meat products — Aerobic count at 30 degrees C (Reference method) |
|
Withdrawn |
1976-04 |
Edition : 1 |
Number of pages : 3 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 2293:1988 |
Meat and meat products — Enumeration of micro-organisms — Colony count technique at 30 degrees C (Reference method) |
|
Withdrawn |
1988-08 |
Edition : 2 |
Number of pages : 4 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 3100-2:1988 |
Meat and meat products — Sampling and preparation of test samples — Part 2: Preparation of test samples for microbiological examination |
The method gives general instructions and specifies procedures to be followed after taking a laboratory sample. This may require the thawing and/or mincing of "open" samples or the pre-incubation, external sterilisation, and aseptic opening of products processed or packed in sealed units.
|
Withdrawn |
1988-06 |
Edition : 1 |
Number of pages : 4 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 3565:1975 |
Meat and meat products — Detection of salmonellae (Reference method) |
|
Withdrawn |
1975-09 |
Edition : 1 |
Number of pages : 11 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 3811:1979 |
Meat and meat products — Detection and enumeration of presumptive coliform bacteria and presumptive Escherichia coli — (Reference method) |
|
Withdrawn |
1979-11 |
Edition : 1 |
Number of pages : 5 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 4031:1978 |
Information interchange — Representation of local time differentials |
|
Withdrawn |
1978-12 |
Edition : 1 |
Number of pages : 2 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 4831:1978 |
Microbiology — General guidance for the enumeration of coliforms — Most probable number technique at 30 degrees C |
|
Withdrawn |
1978-08 |
Edition : 1 |
Number of pages : 8 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 4831:1991 |
Microbiology — General guidance for the enumeration of coliforms — Most probable number technique |
This second edition cancels and replaces the first edition (ISO 4831:1978). It is intended to provide general guidance for the examination of products not dealt with by existing International Standards. These guidelines may not be appropriate for some products in every detail, and for some other products it may be necessary to use different methods. The technique described here is less precise than that described in ISO 4832. Gives references, and the definition of the term coliforms. Specifies principle, culture media and dilution fluid, apparatus and glassware, sampling, preparation of the test sample, procedure, expression of results and test report.
|
Withdrawn |
1991-03 |
Edition : 2 |
Number of pages : 11 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 4831:2006 |
Microbiology of food and animal feeding stuffs — Horizontal method for the detection and enumeration of coliforms — Most probable number technique |
ISO 4831:2006 gives general guidelines for the detection and the enumeration of coliforms. It is applicable to products intended for human consumption and for the feeding of animals, and to environmental samples in the area of food production and food handling.
Enumeration is carried out by calculation of the most probable number (MPN) after incubation in a liquid medium at 30 °C or 37 °C.
This enumeration method is applicable when the number sought is expected to be in the range 1 to 100 per millilitre or per gram of test sample.
|
Published |
2006-08 |
Edition : 3 |
Number of pages : 11 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 4832:1978 |
Microbiology — General guidance for enumeration of coliforms — Colony count technique at 30 degrees C |
|
Withdrawn |
1978-02 |
Edition : 1 |
Number of pages : 4 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 4832:1991 |
Microbiology — General guidance for the enumeration of coliforms — Colony count technique |
This second edition cancels and replaces the first edition (ISO 4832:1978). It is intended to provide general guidance for the examination of products not dealt with by existing International Standards. These guidelines may not be appropriate for some products in every detail, and for some other products it may be necessary to use different methods. Gives references, and the definition of the term coliforms. Specifies principle, culture media and dilution fluid, apparatus and glassware, sampling, preparation of the test sample, procedure, expression of results and test report.
|
Withdrawn |
1991-03 |
Edition : 2 |
Number of pages : 5 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 4832:2006 |
Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of coliforms — Colony-count technique |
ISO 4832:2006 gives general guidelines for the enumeration of coliforms. It is applicable to products intended for human consumption and for the feeding of animals, and environmental samples in the area of food production and food handling, by means of the technique of counting colonies after incubation on a solid medium at 30 °C or at 37 °C.
This technique is recommended when the number of colonies sought is expected to be more than 100 per millilitre or per gram of the test sample.
|
Published |
2006-02 |
Edition : 3 |
Number of pages : 6 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 4833-1:2013 |
Microbiology of the food chain — Horizontal method for the enumeration of microorganisms — Part 1: Colony count at 30 °C by the pour plate technique |
ISO 4833-1:2013 specifies a horizontal method for enumeration of microorganisms that are able to grow and form colonies in a solid medium after aerobic incubation at 30 °C. The method is applicable to: a) products intended for human consumption and for animal feed; b) environmental samples in the area of food and feed production and handling.
ISO 4833-1:2013 is applicable to: 1) products that require a reliable count when a low limit of detection is specified (below 102/g or 102/ml for liquid samples or below 103/g for solid samples); 2) products expected to contain spreading colonies that obscure colonies of other organisms, e.g. milk and milk products likely to contain spreading Bacillus spp.
The applicability of ISO 4833-1:2013 to the examination of certain fermented food and animal feeds is limited and other media or incubation conditions can be more appropriate. However, this method can be applied to such products even though it is possible that the predominant microorganisms in those products are not detected effectively.
For some matrices, the method specified in ISO 4833-1:2013 can give different results to those obtained using the method specified in ISO 4833‑2.
|
Published |
2013-09 |
Edition : 1 |
Number of pages : 9 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 4833-1:2013/Amd 1:2022 |
Microbiology of the food chain — Horizontal method for the enumeration of microorganisms — Part 1: Colony count at 30 °C by the pour plate technique — Amendment 1: Clarification of scope |
|
Published |
2022-01 |
Edition : 1 |
Number of pages : 1 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 4833-2:2013 |
Microbiology of the food chain — Horizontal method for the enumeration of microorganisms — Part 2: Colony count at 30 °C by the surface plating technique |
ISO 4833-2:2013 specifies a horizontal method for enumeration of microorganisms that are able to grow and form colonies on the surface of a solid medium after aerobic incubation at 30 °C. The method is applicable to:
a) products intended for human consumption or for animal feed;
b) environmental samples in the area of food and feed production and food handling.
ISO 4833-2:2013 is applicable to:
1) products containing heat-sensitive organisms that are likely to form a significant proportion of the total flora (e.g. psychrotrophic organisms in chilled and frozen foods, dried foods, other foods that may contain heat-sensitive organisms);
2) products containing obligately aerobic bacteria that are likely to form a significant proportion of the total flora (e.g. Pseudomonas spp.);
3) products that contain small particles that can prove difficult to distinguish from colonies in a pour plate;
4) products whose intense colour prevents the recognition of colonies in a pour plate;
5) products for which distinction between different types of colony is required as part of the assessment of food quality.
In addition to the manual spread plating technique, an annex to ISO 4833-2:2013 also specifies the use of a spiral plater, a rapid method of performing surface colony counts.
The applicability of ISO 4833-2:2013 to the examination of certain fermented food and animal feeds is limited and other media or incubation conditions can be more appropriate. However, this method can be applied to such products even though it is possible that the predominant microorganisms in these products are not detected effectively.
For some matrices, the method described in ISO 4833-2:2013 can give different results to those obtained using the method described in ISO 4833‑1.
|
Published |
2013-09 |
Edition : 1 |
Number of pages : 12 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 4833-2:2013/Amd 1:2022 |
Microbiology of the food chain — Horizontal method for the enumeration of microorganisms — Part 2: Colony count at 30 °C by the surface plating technique — Amendment 1: Clarification of scope |
|
Published |
2022-01 |
Edition : 1 |
Number of pages : 2 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 4833-2:2013/Cor 1:2014 |
Microbiology of the food chain — Horizontal method for the enumeration of microorganisms — Part 2: Colony count at 30 °C by the surface plating technique — Technical Corrigendum 1 |
|
Published |
2014-02 |
Edition : 1 |
Number of pages : 1 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 4833:1978 |
Microbiology — General guidance for enumeration of micro-organisms — Colony count technique at 30 degrees C |
|
Withdrawn |
1978-02 |
Edition : 1 |
Number of pages : 4 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 4833:1991 |
Microbiology — General guidance for the enumeration of micro-organisms — Colony count technique at 30 degrees C |
This second edition cancels and replaces the first edition (ISO 4833:1978). It is intended to provide general guidance for the examination of products not dealt with by existing International Standards. These guidelines may not be appropriate for some products in every detail, and for some other products it may be necessary to use different methods. Gives references, and the definition of the term micro-organisms. Specifies principle, culture media and dilution fluid, apparatus and glassware, sampling, preparation of the test sample, procedure, expression of results and test report.
|
Withdrawn |
1991-03 |
Edition : 2 |
Number of pages : 5 |
Technical Committee |
07.100.01
Microbiology in general
;
07.100.30
Food microbiology
|
| ISO 6887-1:1999 |
Microbiology of food and animal feeding stuffs — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 1: General rules for the preparation of the initial suspension and decimal dilutions |
|
Withdrawn |
1999-02 |
Edition : 1 |
Number of pages : 5 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 4833:2003 |
Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of microorganisms — Colony-count technique at 30 degrees C |
ISO 4833:2003 specifies a horizontal method for the enumeration of microorganisms, by counting the colonies growing in a solid medium after aerobic incubation at 30 degrees Celsius. Subject to some limitations, it is applicable to products intended for human consumption or the feeding of animals.
The applicability of ISO 4833:2003 to the examination of certain fermented food and animal feeding stuffs is limited. For the examination of fermented food and animal feeding stuffs, other media and/or incubation conditions might be more appropriate.
|
Withdrawn |
2003-02 |
Edition : 3 |
Number of pages : 9 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 5541-1:1986 |
Milk and milk products — Enumeration of coliforms — Part 1: Colony count technique at 30 degrees C |
Specifies a method for enumerating bacteria which, at 30 degrees centigrade, form characteristic colonies and which can ferment lactose with the production of gas under the operational conditions described. The method described is applicable to milk and liquid milk products, dried milk, dried sweet whey, dried buttermilk, lactose, acid casein, lactic casein, rennet casein, caseinate, dried acid whey, cheese and processed cheese, butter, frozen milk products, custard, desserts, and cream. Is to be preferred for samples in which large numbers of coliforms are suspected (more than 100 per gram or 10 per millimetre).
|
Withdrawn |
1986-11 |
Edition : 1 |
Number of pages : 7 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 5541-2:1986 |
Milk and milk products — Enumeration of coliforms — Part 2: Most probable number technique at 30 degrees C |
Specifies a method for enumerating bacteria which cause fermentation of lactose with the production of gas and form characteristic growth under the operational conditions describes. Uses a culture technique involving a liquid medium. The calculation of the most probable number is made after incubation at 30 degrees centigrade.
|
Withdrawn |
1986-11 |
Edition : 1 |
Number of pages : 8 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 5552:1979 |
Meat and meat products — Detection and enumeration of Enterobacteriaceae (Reference methods) |
|
Withdrawn |
1979-04 |
Edition : 1 |
Number of pages : 6 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 5552:1997 |
Meat and meat products — Detection and enumeration of Enterobacteriaceae without resuscitation — MPN technique and colony-count technique |
|
Withdrawn |
1997-12 |
Edition : 2 |
Number of pages : 15 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 5944:2001 | IDF 60D: 2001 |
Milk and milk-based products — Detection of coagulase-positive staphylococci — Most probable number technique |
|
Withdrawn |
2001-12 |
Edition : 1 |
Number of pages : 14 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 6391:1988 |
Meat and meat products — Enumeration of Escherichia coli — Colony count technique at 44 degrees C using membranes |
|
Withdrawn |
1988-11 |
Edition : 1 |
Number of pages : 5 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 6391:1997 |
Meat and meat products — Enumeration of Escherichia coli — Colony-count technique at 44 degrees C using membranes |
|
Withdrawn |
1997-12 |
Edition : 2 |
Number of pages : 9 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 6579-1:2017 |
Microbiology of the food chain — Horizontal method for the detection, enumeration and serotyping of Salmonella — Part 1: Detection of Salmonella spp. |
ISO 6579-1:2017 specifies a horizontal method for the detection of Salmonella. It is applicable to the following:
- products intended for human consumption and the feeding of animals;
- environmental samples in the area of food production and food handling;
- samples from the primary production stage such as animal faeces, dust, and swabs.
With this horizontal method, most of the Salmonella serovars are intended to be detected. For the detection of some specific serovars, additional culture steps may be needed. For Salmonella Typhi and Salmonella Paratyphi, the procedure is described in Annex D.
The selective enrichment medium modified semi-solid Rappaport-Vassiliadis (MSRV) agar is intended for the detection of motile Salmonella and is not appropriate for the detection of non-motile Salmonella strains.
|
Published |
2017-02 |
Edition : 1 |
Number of pages : 50 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 6579-1:2017/Amd 1:2020 |
Microbiology of the food chain — Horizontal method for the detection, enumeration and serotyping of Salmonella — Part 1: Detection of Salmonella spp. — Amendment 1: Broader range of incubation temperatures, amendment to the status of Annex D, and correction of the composition of MSRV and SC |
|
Published |
2020-03 |
Edition : 1 |
Number of pages : 11 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 6730:2005 | IDF 101:2005 |
Milk — Enumeration of colony-forming units of psychrotrophic microorganisms — Colony-count technique at 6,5 degrees C |
ISO 6730|IDF 101:2005 specifies a method for the enumeration of colony-forming units of psychrotrophic microorganisms in raw and heat-treated milk by means of the colony-count technique at 6,5 degrees Celsius.
|
Withdrawn |
2005-09 |
Edition : 2 |
Number of pages : 8 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 6785:1985 |
Milk and milk products — Detection of salmonella |
|
Withdrawn |
1985-11 |
Edition : 1 |
Number of pages : 13 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/TS 6579-2:2012 |
Microbiology of food and animal feed — Horizontal method for the detection, enumeration and serotyping of Salmonella — Part 2: Enumeration by a miniaturized most probable number technique |
ISO/TS 6579-2 gives a method for the enumeration of Salmonella spp. present in: products intended for human consumption and for the feeding of animals; environmental samples in the area of food production and food handling; animal faeces; and environmental samples from the primary production stage by calculation of the most probable number (MPN).
The method is based on miniaturization of the dilution, pre-enrichment and selective enrichment steps. The selective enrichment medium, modified semi-solid Rappaport?Vassiliadis (MSRV), is intended for the detection of motile salmonellae and is not appropriate for the detection of non-motile salmonellae.
It is possible that the method is less appropriate to enumerate Salmonella ser. Typhi and Salmonella ser. Paratyphi.
The method is not appropriate for the enumeration of Salmonella spp. in (very) low contaminated samples (<1 cfu/g).
|
Published |
2012-11 |
Edition : 1 |
Number of pages : 18 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/TR 6579-3:2014 |
Microbiology of the food chain — Horizontal method for the detection, enumeration and serotyping of Salmonella — Part 3: Guidelines for serotyping of Salmonella spp. |
ISO/TR 6579-3:2014 gives guidance on the procedure for serotyping Salmonella serovars and is applicable to the serotyping of pure cultures of Salmonella spp., independent of the source from which they are isolated.
|
Published |
2014-07 |
Edition : 1 |
Number of pages : 33 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO/CD TS 6579-4 |
Microbiology of the food chain — Horizontal method for the detection, enumeration and serotyping of Salmonella — Part 4: Identification of monophasic Salmonella Typhimurium (1,4,[5],12:i:-) by polymerase chain reaction (PCR) |
|
Under development |
|
Edition : 1 |
|
Technical Committee |
07.100.30
Food microbiology
|
| ISO 6579:1981 |
Microbiology — General guidance on methods for the detection of Salmonella |
|
Withdrawn |
1981-03 |
Edition : 1 |
Number of pages : 15 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 6579:1993 |
Microbiology — General guidance on methods for the detection of Salmonella |
The methods proposed necessitate four successive stages: pre-enrichment in non-selective liquid medium, enrichment in selective liquid media, plating out and recognition, confirmation. Annex A gives a diagram of the procedure, annex B describes composition and preparation of culture media and reagents, annex C specifies brilliant green.
|
Withdrawn |
1993-09 |
Edition : 3 |
Number of pages : 16 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 6579:2002 |
Microbiology of food and animal feeding stuffs — Horizontal method for the detection of Salmonella spp. |
ISO 6579:2002 specifies a horizontal method for the detection of Salmonella, including Salmonella Typhi and Salmonella Paratyphi.
It is applicable to products intended for human consumption and the feeding of animals, and to environmental samples in the area of food production and food handling.
|
Withdrawn |
2002-07 |
Edition : 4 |
Number of pages : 27 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 6579:2002/Amd 1:2007 |
Microbiology of food and animal feeding stuffs — Horizontal method for the detection of Salmonella spp. — Amendment 1: Annex D: Detection of Salmonella spp. in animal faeces and in environmental samples from the primary production stage |
|
Withdrawn |
2007-07 |
Edition : 4 |
Number of pages : 9 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 6579:2002/Cor 1:2004 |
Microbiology of food and animal feeding stuffs — Horizontal method for the detection of Salmonella spp. — Technical Corrigendum 1 |
|
Withdrawn |
2004-04 |
Edition : 4 |
Number of pages : 1 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 6610:1992 |
Milk and milk products — Enumeration of colony-forming units of micro-organisms — Colony-count technique at 30 degrees C |
Specifies the principle, the diluents and culture medium, the apparatus and glassware, the sampling and procedure, the expression of results, the repeatability and the test report.
|
Withdrawn |
1992-01 |
Edition : 1 |
Number of pages : 5 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 6611:1992 |
Milk and milk products — Enumeration of colony-forming units of yeasts and/or moulds — Colony-count technique at 25 degrees C |
Specifies the principle, the diluents and culture medium, the apparatus and glassware, the sampling and procedure, the expression of results, the repeatability and the test report.
|
Withdrawn |
1992-01 |
Edition : 1 |
Number of pages : 6 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 6611:2004 | IDF 94:2004 |
Milk and milk products — Enumeration of colony-forming units of yeasts and/or moulds — Colony-count technique at 25 degrees C |
ISO 6611|IDF 94:2004 specifies a method for the detection and enumeration of colony-forming units (CFU) of viable yeasts and/or moulds in milk and milk products by means of the colony-count technique at 25 °C.
The method is applicable to milk, liquid milk products, dried milk, dried sweet whey, dried buttermilk, lactose, cheese, acid casein, lactic casein, rennet casein, caseinate, acid whey powder, butter, frozen milk products (including edible ices), custard, desserts, fermented milk and cream.
|
Published |
2004-10 |
Edition : 2 |
Number of pages : 8 |
Technical Committee |
07.100.30
Food microbiology
;
67.100.01
Milk and milk products in general
|
| ISO 6887-1:2017 |
Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 1: General rules for the preparation of the initial suspension and decimal dilutions |
ISO 6887-1:2017 defines general rules for the aerobic preparation of the initial suspension and of dilutions for microbiological examinations of products intended for human or animal consumption.
ISO 6887-1:2017 is applicable to the general case and other parts apply to specific groups of products as mentioned in the foreword. Some aspects might also be applicable to molecular methods where matrices can be associated with inhibition of the PCR steps and consequently affect the test result.
ISO 6887-1:2017 excludes preparation of samples for both enumeration and detection test methods where preparation instructions are detailed in specific International Standards.
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Published |
2017-03 |
Edition : 2 |
Number of pages : 26 |
Technical Committee |
07.100.30
Food microbiology
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| ISO 6887-1:2017/CD Amd 1 |
Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 1: General rules for the preparation of the initial suspension and decimal dilutions — Amendment 1: Requirements and guidance on the use of larger test portion size for qualitative methods |
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Under development |
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Edition : 2 |
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Technical Committee |
07.100.30
Food microbiology
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| ISO 6887-2:2003 |
Microbiology of food and animal feeding stuffs — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 2: Specific rules for the preparation of meat and meat products |
ISO 6887-2:2003 specifies rules for the preparation of meat and meat product samples and their suspension for microbiological examination when the samples require a different preparation from the method described in ISO 6887-1. ISO 6887-1 defines the general rules for the preparation of the initial suspension and decimal dilutions for microbiological examination. ISO 6887-2:2003 only describes methods of preparation that are applicable to several microorganisms simultaneously. It excludes the preparations that only apply to the detection and/or enumeration of a single microorganism where the method of preparation is described in the relevant International Standard concerning that microorganism.
ISO 6887-2:2003 is applicable to the following fresh, raw and processed meats and poultry and their products: refrigerated or frozen; cured or fermented; minced or comminuted; delicatessen meats; pre-cooked meals or poultry-based meals; dried and smoked meats at various degrees of dehydration; concentrated meat extracts.
ISO 6887-2:2003 is not applicable to the following products whose microbiological examination is described in other International Standards: canned products; other products (see ISO 6887-4).
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Withdrawn |
2003-07 |
Edition : 1 |
Number of pages : 15 |
Technical Committee |
07.100.30
Food microbiology
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| ISO 6887-2:2017 |
Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 2: Specific rules for the preparation of meat and meat products |
ISO 6887-2:2017 specifies rules for the preparation of meat and meat product samples and their suspension for microbiological examination when the samples require different preparation from the methods described in ISO 6887‑1. ISO 6887‑1 defines the general rules for the preparation of the initial suspension and dilutions for microbiological examination.
ISO 6887-2:2017 excludes preparation of samples for both enumeration and detection test methods where preparation details are specified in the relevant International Standards.
ISO 6887-2:2017 is applicable to the following fresh, raw and processed meats, poultry and game and their products:
- refrigerated or frozen;
- cured or fermented;
- minced or comminuted;
- meat preparations;
- mechanically separated meat;
- cooked meats;
- dried and smoked meats at various degrees of dehydration;
- concentrated meat extracts;
- excision and swab samples from carcasses.
ISO 6887-2:2017 excludes the sampling of carcasses (see ISO 17604) and preparation of samples from the primary production stage (see ISO 6887‑6).
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Published |
2017-03 |
Edition : 2 |
Number of pages : 9 |
Technical Committee |
07.100.30
Food microbiology
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| ISO 6887-3:2003 |
Microbiology of food and animal feeding stuffs — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 3: Specific rules for the preparation of fish and fishery products |
ISO 6887-3:2003 specifies rules for the preparation of fish and fishery product samples and their suspension for microbiological examination when the samples require a different preparation from the method described in ISO 6887-1. ISO 6887-1 defines the general rules for the preparation of the initial suspension and decimal dilutions for microbiological examination.
ISO 6887-3:2003 only describes methods of preparation that are applicable to several microorganisms simultaneously. It excludes the preparations that only apply to the detection and/or enumeration of a single microorganism where the methods of preparation are described in the relevant standard concerning that microorganism, for example Vibrio parahaemolyticus.
ISO 6887-3:2003 is applicable to the following raw, processed, cooked or frozen fish and shellfish and their products: raw fish, crustaceans, molluscs and others; processed fish, crustaceans, molluscs and others; frozen fish, crustaceans, molluscs and others, in blocks or otherwise.
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Withdrawn |
2003-08 |
Edition : 1 |
Number of pages : 11 |
Technical Committee |
07.100.30
Food microbiology
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| ISO 6888-2:1999 |
Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) — Part 2: Technique using rabbit plasma fibrinogen agar medium |
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Withdrawn |
1999-02 |
Edition : 1 |
Number of pages : 7 |
Technical Committee |
07.100.30
Food microbiology
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| ISO 16654:2001/Amd 2:2023 |
Microbiology of food and animal feeding stuffs — Horizontal method for the detection of Escherichia coli O157 — Amendment 2: Inclusion of performance testing of all culture media and reagents |
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Published |
2023-01 |
Edition : 1 |
Number of pages : 2 |
Technical Committee |
07.100.30
Food microbiology
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| ISO 6887-3:2017 |
Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 3: Specific rules for the preparation of fish and fishery products |
ISO 6887-3:2017 specifies rules for the preparation of fish and fishery product samples and their suspension for microbiological examination when the samples require a different preparation from the methods described in ISO 6887‑1. ISO 6887‑1 defines the general rules for the preparation of the initial suspension and dilutions for microbiological examination.
ISO 6887-3:2017 includes special procedures for sampling raw molluscs, tunicates and echinoderms from primary production areas.
NOTE 1 Sampling of raw molluscs, tunicates and echinoderms from primary production areas is included in this document, rather than ISO 13307, which specifies rules for sampling from the terrestrial primary production stage.
ISO 6887-3:2017 excludes preparation of samples for both enumeration and detection test methods where preparation details are specified in the relevant International Standards (e.g. ISO/TS 15216‑1 and ISO/TS 15216‑2 for determination of hepatitis A virus and norovirus in food using real-time RT-PCR).
ISO 6887-3:2017 is intended to be used in conjunction with ISO 6887‑1. It is applicable to the following raw, processed or frozen fish and shellfish and their products (see Annex A for classification of major taxa):
a) Raw fishery products, molluscs, tunicates and echinoderms including:
- whole fish or fillets, with or without skin and heads, and gutted;
- crustaceans, whole or shelled;
- cephalopods;
- bivalve molluscs;
- gastropods;
- tunicates and echinoderms.
b) Processed products including:
- smoked fish, whole or prepared fillets, with or without skin;
- cooked or partially cooked, whole or shelled crustaceans, molluscs, tunicates and echinoderms;
- cooked or partially cooked fish and fish-based multi-component products.
c) Raw or cooked frozen fish, crustaceans, molluscs and others, in blocks or otherwise, including:
- fish, fish fillets and pieces;
- whole and shelled crustacean (e.g. flaked crab, prawns), molluscs, tunicates and echinoderms.
NOTE 2 The purpose of examinations performed on these samples can be either hygiene testing or quality control. However, the sampling techniques described in this document relate mainly to hygiene testing (on muscle tissues).
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Published |
2017-03 |
Edition : 2 |
Number of pages : 16 |
Technical Committee |
07.100.30
Food microbiology
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| ISO 6887-3:2017/Amd 1:2020 |
Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 3: Specific rules for the preparation of fish and fishery products — Amendment 1: Sample preparation for raw marine gastropods |
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Published |
2020-02 |
Edition : 2 |
Number of pages : 1 |
Technical Committee |
07.100.30
Food microbiology
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| ISO 6887-4:2003 |
Microbiology of food and animal feeding stuffs — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 4: Specific rules for the preparation of products other than milk and milk products, meat and meat products, and fish and fishery products |
ISO 6887-4:2003 specifies rules for the preparation of samples and decimal dilutions for the microbiological examination of food products other than those covered in other parts of ISO 6887. ISO 6887-1 defines the general rules for the preparation of the initial suspension and decimal dilutions for microbiological examination.
ISO 6887-4:2003 only describes methods of preparation that are applicable to several microorganisms simultaneously. It excludes the preparations that only apply to the detection and/or enumeration of a single microorganism where the methods of preparation are described in the relevant International Standard concerning that microorganism.
ISO 6887-4:2003 is applicable to the following products: general case for acidic products; foods with a high fat content, excluding margarine and spreads; flours, whole cereal grains, cereal by-products, animal feeds and cattle cake; very hard products; gelatine; margarine and spreads; dehydrated products and freeze-dried products (except dairy products and egg products); egg and egg products; fermented products (products containing live microorganisms); pastries and cakes.
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Withdrawn |
2003-08 |
Edition : 1 |
Number of pages : 13 |
Technical Committee |
07.100.30
Food microbiology
|
| ISO 6887-4:2003/Amd 1:2011 |
Microbiology of food and animal feeding stuffs — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 4: Specific rules for the preparation of products other than milk and milk products, meat and meat products, and fish and fishery products — Amendment 1 |
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Withdrawn |
2011-10 |
Edition : 1 |
Number of pages : 2 |
Technical Committee |
07.100.30
Food microbiology
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| ISO 6887-4:2003/Cor 1:2004 |
Microbiology of food and animal feeding stuffs — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 4: Specific rules for the preparation of products other than milk and milk products, meat and meat products, and fish and fishery products — Technical Corrigendum 1 |
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Withdrawn |
2004-10 |
Edition : 1 |
Number of pages : 1 |
Technical Committee |
07.100.30
Food microbiology
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| ISO 6887-4:2017 |
Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 4: Specific rules for the preparation of miscellaneous products |
ISO 6887-4:2017 specifies rules for the preparation of samples and dilutions for the microbiological examination of specific food products not covered in other parts of ISO 6887, which deal with more general categories. This document covers a wide range of miscellaneous products, but does not include new products brought on to the market after publication.
ISO 6887‑1 defines the general rules for the preparation of the initial suspension and dilutions for microbiological examination.
ISO 6887-4:2017 excludes preparation of samples for both enumeration and detection test methods when preparation details are specified in the relevant International Standards.
ISO 6887-4:2017 is applicable to the following products:
- acidic (low pH) products;
- hard and dry products;
- dehydrated, freeze-dried and other low aw products (including those with inhibitory properties);
- flours, whole cereal grains, cereal by-products;
- animal feed, cattle cake, kibbles and pet chews;
- gelatine (powdered and leaf);
- margarines, spreads and non-dairy products with added water;
- eggs and egg products;
- bakery goods, pastries and cakes;
- fresh fruit and vegetables;
- fermented products and other products containing viable microorganisms;
- alcoholic and non-alcoholic beverages;
- alternative protein products.
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Published |
2017-03 |
Edition : 2 |
Number of pages : 16 |
Technical Committee |
07.100.30
Food microbiology
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